Antibody purification involves isolation of antibody from serum (polyclonal antibody), ascites fluid, or from the culture supernatant of a hybridoma cell line (monoclonal antibody). Protein G is a recombinant protein expressed in Escherichia coli (21.6 kDa; 2 IgG binding sites) and is recommended for purifying IgG from mouse, human, cow, goat, or sheep serum. The POROS™ MabCapture™ G Select Resin is a new product that can be scaled up for bioprocess applications.

We offer a wide selection of base supports and formats to purify antibodies using the Protein G ligand. Our portfolio is designed to meet small-scale (screening) up to large-scale (bioprocess) needs.

  • Multiple formats—magnetic beads, loose resin, spin columns and kits, FPLC cartridges, and 96-well filter plates enable antibody purification from microgram to kilogram scales
  • High performance—resins are designed to maximize protein yield and reduce background
  • Economical—pricing is similar to or better than our leading competitors

Choose the right Protein G product for antibody purification

Product links in the last row of each column

 Dynabeads Protein G Magnetic BeadsPierce Protein G AgarosePierce Protein G Plus AgarosePOROS MabCapture G Select
Bead or resin size2.8 μm45–165 μm45–165 μm50 μm
Binding capacity250-300 ug human IgG/mL beads11–15 mg human IgG/mL resin≥20 mg human IgG/mL resin≥13 mg/mL human IgG/mL resin
Maximum linear flow rateN/A700 cm/hr700 cm/hr3000 cm/hr
SupportMonosized, super paramagnetic polystyrene beadsCrosslinked 6% beaded agaroseCrosslinked 6% beaded agarosePolystyrene-divinylbenzene
Recommended application scalescreeningbatch, pilotbatch, pilotbatch, pilot, process
Formats availableloose beadsloose beads, pre-packed spin columns and kits, chromatography cartridges, spin platesloose beadsloose beads
Order product(s)10003D20398
(loose resin)
(1 mL cartridge)
(5 mL cartridge)
(spin plates)
(loose resin)