Antibody purification involves isolation of antibody from serum (polyclonal antibody), ascites fluid, or from the culture supernatant of a hybridoma cell line (monoclonal antibody). Protein G is a recombinant protein expressed in Escherichia coli (21.6 kDa; 2 IgG binding sites) and is recommended for purifying IgG from mouse, human, cow, goat, or sheep serum. The POROS™ MabCapture™ G Select Resin is a new product that can be scaled up for bioprocess applications.

We offer a wide selection of base supports and formats to purify antibodies using the Protein G ligand. Our portfolio is designed to meet small-scale (screening) up to large-scale (bioprocess) needs.

  • Multiple formats—magnetic beads, loose resin, spin columns and kits, FPLC cartridges, and 96-well filter plates enable antibody purification from microgram to kilogram scales
  • High performance—resins are designed to maximize protein yield and reduce background
  • Economical—pricing is similar to or better than our leading competitors

Choose the right Protein G product for antibody purification

Product links in the last row of each column

  Dynabeads Protein G Magnetic Beads Pierce Protein G Agarose Pierce Protein G Plus Agarose POROS MabCapture G Select
Bead or resin size 2.8 μm 45–165 μm 45–165 μm 50 μm
Binding capacity 25–30 µg human IgG/mg beads 11–15 mg human IgG/mL resin ≥20 mg human IgG/mL resin ≥13 mg/mL human IgG/mL resin
Maximum linear flow rate N/A 700 cm/hr 700 cm/hr 3000 cm/hr
Support Monosized, super paramagnetic polystyrene beads Crosslinked 6% beaded agarose Crosslinked 6% beaded agarose Polystyrene-divinylbenzene
Recommended application scale screening batch, pilot batch, pilot batch, pilot, process
Formats available loose beads loose beads, pre-packed spin columns and kits, chromatography cartridges, spin plates loose beads loose beads
Order product(s) 10003D 20398
(loose resin)
89926
(1 mL cartridge)
89927
(5 mL cartridge)
45204
(spin plates)
22851
(loose resin)
82082