Antibody Purification Using Protein L
Antibody purification involves isolation of antibody from serum (polyclonal antibody), ascites fluid, or from the culture supernatant of a hybridoma cell line (monoclonal antibody). Protein L is a recombinant protein expressed in Escherichia coli (35.8 kDa; 4 IgG binding sites) and is recommended for human or mouse monoclonal antibodies known to have appropriate kappa light chains. The POROS™ MabCapture™ L Select Resin is a new product that can be scaled up for bioprocess applications.
We offer two base supports and a variety of formats to purify antibodies using the Protein L ligand. Our portfolio is designed to meet small-scale (screening) to pilot-scale needs.
- Multiple formats—magnetic beads, loose resin, spin columns and kits, and FPLC cartridges enable antibody purification from microgram to kilogram scales
- High performance—resins are designed to maximize protein yield and reduce background
- Economical—pricing is similar to or better than our leading competitors
Choose the right Protein L product for antibody purification
Product links in the last row of each column
|Pierce Protein L
|Pierce Protein L
|Pierce Protein L Plus
|Bead or resin size||1 μm||45–165 μm||45–165 μm|
|Binding capacity||≥110 μg human IgG/mg beads||5–10 mg human IgG/mL resin||10–20 mg human IgG/mL resin|
|Maximum linear flow rate||N/A||700 cm/hr||700 cm/hr|
|Support||Magnetite coated polymer||Crosslinked 6% beaded agarose||Crosslinked 6% beaded agarose|
|Recommended application scale||screening||batch, pilot||batch, pilot|
|Formats available||loose beads||loose beads, pre-packed spin columns and kits, chromatography cartridges||loose beads|
(1 mL cartridge)
(5 mL cartridge)
For Research Use Only. Not for use in diagnostic procedures.