Antibody purification involves isolation of antibody from serum (polyclonal antibody), ascites fluid, or from the culture supernatant of a hybridoma cell line (monoclonal antibody). Protein L is a recombinant protein expressed in Escherichia coli (35.8 kDa; 4 IgG binding sites) and is recommended for human or mouse monoclonal antibodies known to have appropriate kappa light chains.

We offer two base supports and a variety of formats to purify antibodies using the Protein L ligand. Our portfolio is designed to meet small-scale (screening) to pilot-scale needs.

  • Multiple formats—magnetic beads, loose resin, spin columns and kits, and FPLC cartridges enable antibody purification from microgram to kilogram scales
  • High performance—resins are designed to maximize protein yield and reduce background
  • Economical—pricing is similar to or better than our leading competitors

Choose the right Protein L product for antibody purification

Product links in the last row of each column

  Pierce Protein L
Magnetic Beads
Pierce Protein L
Pierce Protein L Plus
Bead or resin size 1 μm 45–165 μm 45–165 μm
Binding capacity ≥110 μg human IgG/mg beads 5–10 mg human IgG/mL resin 10–20 mg human IgG/mL resin
Maximum linear flow rate N/A 700 cm/hr 700 cm/hr
Support Magnetite coated polymer Crosslinked 6% beaded agarose Crosslinked 6% beaded agarose
Recommended application scale screening batch, pilot batch, pilot
Formats available loose beads loose beads, pre-packed spin columns and kits, chromatography cartridges loose beads
Order product(s) 88849 20510
(loose resin)
(1 mL cartridge)
(5 mL cartridge)
(loose resin)