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We offer versatile options for antibody purification of monoclonal antibodies including in vitro and in vivo ascites hybridoma production options for monoclonal antibodies. We also provide many service options for purifying the monoclonal antibodies that have been produced by these methods.

Following development of antibody-generating hybridomas, monoclonal antibodies can be produced from them by either (1) cell culture in t-flasks or bioreactors to yield antibody-containing supernatant, or (2) mouse abdominal culture to yield antibody-containing ascites fluid. Each liter of cell culture yields approximately 10 to 30mg of antibody. Each mouse abdominal culture yields 2 to 10mg of antibody in 2 to 3mL of neat ascites fluid.

Several options are available for monoclonal antibody purification from supernatants or ascites. Most customers prefer simple affinity purification with Protein A or Protein G resin, but we also can purify using anti-immunoglobulin antibodies, or immobilized antigen.

Purification options for monoclonal antibodies

Monoclonal supernatants are purified under high salt conditions for stringent binding pending the antibody species and isotype. When the affinity of the antibody for an indicated capture resin is not known, small-scale pilots can be performed at additional costs.

  • Standard Purifications (included with in vitro production):
    • Protein A/G or anti-immunoglobulin resin
    • Protein A/G or anti-immunoglobulin resin with low endotoxin
  • GMP Purifications: Specialized GMP purifications are available for customers who require complete traceability and process-validation.

Antibody labeling

We also provide integrated services for chemically labeling purified monoclonal antibodies to create conjugated antibodies for use in most assay system. Let us know your needs and we can include this option when we provide a project quotation.

  • Biotin
  • HRP (peroxidase)
  • AP (alk-phos)
  • DyLight Fluors
  • FITC
  • TRITC
  • PE
  • Rhodamine
  • Streptavidin
  • APC
  • Agarose
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The Thermo Scientific Antigen Profiler is a bioinformatics protein sequence analysis tool and custom peptide design algorithm for designing and creating superior peptide antigens. The platform incorporates an exclusive set of powerful bioinformatics algorithms for analyzing and designing peptide and protein antigens to ensure high-titer custom production of highly-specific antibodies. The analysis is offered as a service for researchers who wish to design and synthesize effective peptide antigens for custom antibody development using our comprehensive custom antibody production service.

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