Following development of antibody-generating hybridomas, monoclonal antibodies can be produced from them by either (1) cell culture in T-flasks or bioreactors to yield antibody-containing supernatant or (2) mouse abdominal culture to yield antibody-containing ascites fluid. Each liter of cell culture typically yields approximately 10 to 30 mg of antibody. Each mouse abdominal culture yields 2 to 10 mg of antibody in 2 to 3 mL of neat ascites fluid.
Several options are available for monoclonal antibody purification from supernatants or ascites. Most customers prefer simple affinity purification with Protein A or Protein G resin, but we also can purify using anti-immunoglobulin antibodies or immobilized antigen.
Purification options for monoclonal antibodies
Monoclonal supernatants are purified under high salt conditions for stringent binding pending the antibody species and isotype. When the affinity of the antibody for an indicated capture resin is not known, small-scale pilots can be performed at additional costs.
- Standard purifications (included with in vitro production):
- Protein A/G or anti-immunoglobulin resin
- Protein A/G or anti-immunoglobulin resin with low endotoxin
We also provide integrated services for chemically labeling purified monoclonal antibodies to create conjugated antibodies for use in most assay systems. Let us know your needs and we can include this option when we provide a project quotation.
- HRP (peroxidase)
- AP (alk-phos)
- DyLight Fluors
The Thermo Scientific Antigen Profiler is a bioinformatics protein sequence analysis tool and custom peptide design algorithm for designing and creating superior peptide antigens. The platform incorporates an exclusive set of powerful bioinformatics algorithms for analyzing and designing peptide and protein antigens to ensure high-titer custom production of highly-specific antibodies. The analysis is offered as a service for researchers who wish to design and synthesize effective peptide antigens for custom antibody development using our comprehensive custom antibody production service.
Choose the custom antibody services based on specific assay applications and overall price.
Custom antibody services for production and purification of modification-specific antibodies.
ELISA and additional screening and method validation procedures for custom antibodies.
Tools and information
For Research Use Only. Not for use in diagnostic procedures.