Search from over 1000 highly-referenced ELISA kits

ELISA Kits

High-quality optimized Invitrogen ELISA kits allow you to measure target-specific proteins with confidence, reliability, and consistency. A variety of ELISA kit formats are available which include complete, ready-to-use kits as well as preoptimized reagents to design your own. ELISA kits and antibody pairs are available for a range of different species including human, mouse, rat, nonhuman primate, canine, porcine, bovine, equine, and feline. In addition to the off-the-shelf ELISA formats, special services such as: lot reservation, bulk packaging and custom target development are available for flexibility and convenience as you start your next research project. Search all the kits available with the search bar above.

ELISA kits available for specific research areas

Immune monitoring

Find ELISA kits that detect a broad range of secreted, circulating cytokines, chemokines, and growth factors for immunology and inflammation research.

Immuno-Oncology

Immuno-Oncology Invitrogen ELISA kits allow the research of soluble immune checkpoint molecules and help to provide a comprehensive picture of cancer immunity.

Neurobiology

Invitrogen offers high-quality ELISA kits for studying neurological disorders or neuroscience-related protein targets.

Phospho-specific

Phosphorylation-specific Invitrogen ELISA kits are designed for studying intracellular proteins involved in signaling pathways.

Immunoglobulin

Immunoglobulin-specific and isotyping Invitrogen ELISA kits provide a convenient method to identify and quantify antibody classes, subclasses, and isotypes.

Invitrogen ELISA formats

  Uncoated and antibody pair kits Coated ELISA kits* Instant ELISA kits ProQuantum kits
Overnight coating required Yes No, pre-coated plate No, pre-coated plate No coating required
Analytical sensitivity** <5 pg/ml <5 pg/ml <5 pg/ml <1 pg/mL
Dynamic range** <7.8-500 pg/ml <7.8-500 pg/ml <7.8-500 pg/ml <1.6-5,000 pg/mL
Sample volume 10-100 µL 10-100 µL 25-50 µL 2-5 µL
Hands-on time 1 hr 45 mins 1 hr 20 mins 20 mins 30 mins
Incubation time** Overnight 2.5-4 hr 4 hr 1 hr
Instrumentation needed Microplate reader, absorbance Microplate reader, absorbance Microplate reader, absorbance qPCR instrument

*Values in this table refer to our Standard Colorimetric kits. Ultrasensitive kits are also available.
**Every assay has its own specifications. Please consult the protocol for your specific immunoassays/kits.

Precoated ELISA kits

Invitrogen pre-coated ELISA kits are ready-to-use and have been tested to provide the lowest possible inter- and intra-assay variability and the highest possible lot-to-lot consistency. ELISA kits with precoated plates help ensure that you obtain consistent ELISA performance and are designed for labs that require validated and comprehensively tested kits.

Learn more about our ELISA kit validation and quality testing

Benefits of precoated ELISA Kits

  • Validated performance⁠—tested for plate variability (CV) and final kit validation testing before shipping out for complete confidence.
  • Easy-to-use⁠—includes all the required assay buffers and reagents, and a straightforward protocol.
  • Fast⁠—pre-coated plates allow for a workflow typically completed within 4 hours.

Included kit components

  • Precoated 96-well plate
  • Pretitrated capture antibody
  • Pretitrated detection antibody
  • Recombinant protein standard
  • Stop solution
  • Substrate solution
  • Assay buffer (sample diluent)
  • Wash buffer

Using Invitrogen pre-coated ELISA kits

View the instructional video showing how to use Invitrogen pre-coated ready-to-use ELISA kits. Please make sure to carefully read and review your ELISA kit’s specific instruction sheet, located inside the package, for detailed information before beginning the procedure.

Uncoated ELISA and antibody pair kits

Invitrogen uncoated and matched antibody pair ELISA kits have the reagents needed to coat-your-own plates, overnight process, and run the assay. Kit configurations provide easy-to-use, flexible options. Uncoated kits and antibody pair kits are ideal for experienced ELISA users on a budget with less stringent requirements for inter- and intra- assay variance.

Benefits of Uncoated ELISA kits

  • Pre-titered and optimized—matched antibody pairs and all provided reagents optimized for ELISA development.
  • Most options for kit configuration—purchase based on the volume of your assay through a choice of package sizes, including 2-, 10- and 20-plate kits.
  • Complete—includes all the essential reagents and buffers to limit variability. Generic reagents like wash and stop solution not included.
  • Affordable—prices to accommodate the most demanding budgets and to help maximize your research.

Included kit components

  • Pretitrated capture antibody
  • Pretitrated detection antibody
  • Recombinant protein standard
  • Substrate solution
  • Assay buffer (sample diluent)
  • Coating buffer

Benefits of antibody pair kits

  • Flexible—matched antibody pairs can be used for ELISA or with other immunoassay platforms.
  • Convenient packaging option—All-in-one buffer kit sold separately, if needed for ELISA development.

How to coat a plate and use Invitrogen uncoated ELISA kits

View the instructional video showing how to coat a plate and use Invitrogen uncoated ELISA kits. Please make sure to carefully read and review your ELISA kit’s specific instruction sheet, located inside the package, for detailed information before beginning the procedure.

Instant ELISA kits

Invitrogen Instant ELISA Kits have a condensed workflow format designed to alleviate the inconveniences of multiple preparation and incubation steps. In conventional ELISA kits, the precoated plates provide only the capture antibody, to which the sample and detection reagents must be added sequentially. In contrast, Instant ELISA Kits come with ready-to-use plates that contain all necessary assay components—including capture antibody and lyophilized detection antibody, streptavidin-HRP, and sample diluent. These prepared plates greatly reduce pipetting time and enable a simple one-wash protocol. Furthermore, serially diluted, lyophilized protein standards are provided in additional strip wells, eliminating the need for plate setup or serial dilutions to create a standard curve and leaving less room for error.
Illustration showing the steps for the Invitrogen Instant ELISA Assay compared to conventional ELISAs
 Click image to enlarge
Comparison of Instant ELISA technology vs. conventional ELISA procedures. With Instant ELISA kits, all the preparation work has been done for you, thereby decreasing hands-on time per experiment. In addition, Instant ELISA kits come with a full 96-well plate for your samples and two separately packed standards. This enables the use of all 96 wells for your samples expanding your ELISA capacity by 20%.
Comparison of the list of 7 steps for Invitrogen Instant ELISA Kits and the list of 17 steps for conventional ELISA protocols.
 Click image to enlarge
Fewer steps help to save time and reduce variation. There are only 7 working steps when using an Instant ELISA kit, as opposed to 17 steps when performing a conventional ELISA. Therefore, you can decrease total hands-on time per experiment to about 1 hour by adopting the Instant ELISA technique.

Instant ELISA Kits

  • No preparation of reagents
  • No serial dilutions of standards
  • No sequential additions to the plate

Included kit components

  • Precoated 96-well plate
  • Pretitrated capture antibody
  • Pretitrated detection antibody
  • Recombinant protein standard (in additional well strips)
  • Stop solution
  • Substrate solution
  • Assay buffer (sample diluent)
  • Wash buffer
  • Lysis buffer

ProQuantum Kits- high-sensitivity immunoassays

ProQuantum immunoassays utilize a matched pair of target-specific antibodies each conjugated to a DNA oligonucleotide. This platform leverages the sensitivity and large dynamic range of Applied Biosystems TaqMan real-time PCR technology. During antibody–analyte binding, the two DNA oligos are brought into close proximity, which then allows for ligation of the two strands and subsequent creation of template strand for amplification.

Illustration showing matched antibody pair in the ProQuantum assay followed by the PCR amplification of signal that occurs when two antibodies are proimate to each other.
 Click image to enlarge

ProQuantum high-senstivity immunoassay schematic. (A) Antibody-conjugates binding to target (1-hr incubation). (B) Ligation and amplification of signal (in qPCR instrument).

Benefits of ProQuantum immunoassays kits

  • High-sensitivity—detect low levels of proteins with greater sensitivity than traditional ELISA methods.
  • Broad dynamic range—≥5 logarithmic units, minimizing sample dilutions to ensure they fall within the range.
  • Small sample consumption—use 2–5 μL of sample (instead of 75 μL for triplicate wells with other ELISA methods).
  • Fast, easy workflow—typically 2 hours from sample to answer, no wash steps, and a single 1-hour incubation.
  • No proprietary instrument to purchase—runs on any real-time PCR instrument.
  • Includes intuitive cloud-based softwarecomprehensive data analysis and statistical group–wise comparison.

How to use a ProQuantum high-sensitivity immunoassay kit

Watch this instructional video on how to run a ProQuantum assay to see the simple steps to run the assay at your lab bench.

Invitrogen ELISA formats

  Uncoated and antibody pair kits Coated ELISA kits* Instant ELISA kits ProQuantum kits
Overnight coating required Yes No, pre-coated plate No, pre-coated plate No coating required
Analytical sensitivity** <5 pg/ml <5 pg/ml <5 pg/ml <1 pg/mL
Dynamic range** <7.8-500 pg/ml <7.8-500 pg/ml <7.8-500 pg/ml <1.6-5,000 pg/mL
Sample volume 10-100 µL 10-100 µL 25-50 µL 2-5 µL
Hands-on time 1 hr 45 mins 1 hr 20 mins 20 mins 30 mins
Incubation time** Overnight 2.5-4 hr 4 hr 1 hr
Instrumentation needed Microplate reader, absorbance Microplate reader, absorbance Microplate reader, absorbance qPCR instrument

*Values in this table refer to our Standard Colorimetric kits. Ultrasensitive kits are also available.
**Every assay has its own specifications. Please consult the protocol for your specific immunoassays/kits.

Precoated ELISA kits

Invitrogen pre-coated ELISA kits are ready-to-use and have been tested to provide the lowest possible inter- and intra-assay variability and the highest possible lot-to-lot consistency. ELISA kits with precoated plates help ensure that you obtain consistent ELISA performance and are designed for labs that require validated and comprehensively tested kits.

Learn more about our ELISA kit validation and quality testing

Benefits of precoated ELISA Kits

  • Validated performance⁠—tested for plate variability (CV) and final kit validation testing before shipping out for complete confidence.
  • Easy-to-use⁠—includes all the required assay buffers and reagents, and a straightforward protocol.
  • Fast⁠—pre-coated plates allow for a workflow typically completed within 4 hours.

Included kit components

  • Precoated 96-well plate
  • Pretitrated capture antibody
  • Pretitrated detection antibody
  • Recombinant protein standard
  • Stop solution
  • Substrate solution
  • Assay buffer (sample diluent)
  • Wash buffer

Using Invitrogen pre-coated ELISA kits

View the instructional video showing how to use Invitrogen pre-coated ready-to-use ELISA kits. Please make sure to carefully read and review your ELISA kit’s specific instruction sheet, located inside the package, for detailed information before beginning the procedure.

Uncoated ELISA and antibody pair kits

Invitrogen uncoated and matched antibody pair ELISA kits have the reagents needed to coat-your-own plates, overnight process, and run the assay. Kit configurations provide easy-to-use, flexible options. Uncoated kits and antibody pair kits are ideal for experienced ELISA users on a budget with less stringent requirements for inter- and intra- assay variance.

Benefits of Uncoated ELISA kits

  • Pre-titered and optimized—matched antibody pairs and all provided reagents optimized for ELISA development.
  • Most options for kit configuration—purchase based on the volume of your assay through a choice of package sizes, including 2-, 10- and 20-plate kits.
  • Complete—includes all the essential reagents and buffers to limit variability. Generic reagents like wash and stop solution not included.
  • Affordable—prices to accommodate the most demanding budgets and to help maximize your research.

Included kit components

  • Pretitrated capture antibody
  • Pretitrated detection antibody
  • Recombinant protein standard
  • Substrate solution
  • Assay buffer (sample diluent)
  • Coating buffer

Benefits of antibody pair kits

  • Flexible—matched antibody pairs can be used for ELISA or with other immunoassay platforms.
  • Convenient packaging option—All-in-one buffer kit sold separately, if needed for ELISA development.

How to coat a plate and use Invitrogen uncoated ELISA kits

View the instructional video showing how to coat a plate and use Invitrogen uncoated ELISA kits. Please make sure to carefully read and review your ELISA kit’s specific instruction sheet, located inside the package, for detailed information before beginning the procedure.

Instant ELISA kits

Invitrogen Instant ELISA Kits have a condensed workflow format designed to alleviate the inconveniences of multiple preparation and incubation steps. In conventional ELISA kits, the precoated plates provide only the capture antibody, to which the sample and detection reagents must be added sequentially. In contrast, Instant ELISA Kits come with ready-to-use plates that contain all necessary assay components—including capture antibody and lyophilized detection antibody, streptavidin-HRP, and sample diluent. These prepared plates greatly reduce pipetting time and enable a simple one-wash protocol. Furthermore, serially diluted, lyophilized protein standards are provided in additional strip wells, eliminating the need for plate setup or serial dilutions to create a standard curve and leaving less room for error.
Illustration showing the steps for the Invitrogen Instant ELISA Assay compared to conventional ELISAs
 Click image to enlarge
Comparison of Instant ELISA technology vs. conventional ELISA procedures. With Instant ELISA kits, all the preparation work has been done for you, thereby decreasing hands-on time per experiment. In addition, Instant ELISA kits come with a full 96-well plate for your samples and two separately packed standards. This enables the use of all 96 wells for your samples expanding your ELISA capacity by 20%.
Comparison of the list of 7 steps for Invitrogen Instant ELISA Kits and the list of 17 steps for conventional ELISA protocols.
 Click image to enlarge
Fewer steps help to save time and reduce variation. There are only 7 working steps when using an Instant ELISA kit, as opposed to 17 steps when performing a conventional ELISA. Therefore, you can decrease total hands-on time per experiment to about 1 hour by adopting the Instant ELISA technique.

Instant ELISA Kits

  • No preparation of reagents
  • No serial dilutions of standards
  • No sequential additions to the plate

Included kit components

  • Precoated 96-well plate
  • Pretitrated capture antibody
  • Pretitrated detection antibody
  • Recombinant protein standard (in additional well strips)
  • Stop solution
  • Substrate solution
  • Assay buffer (sample diluent)
  • Wash buffer
  • Lysis buffer

ProQuantum Kits- high-sensitivity immunoassays

ProQuantum immunoassays utilize a matched pair of target-specific antibodies each conjugated to a DNA oligonucleotide. This platform leverages the sensitivity and large dynamic range of Applied Biosystems TaqMan real-time PCR technology. During antibody–analyte binding, the two DNA oligos are brought into close proximity, which then allows for ligation of the two strands and subsequent creation of template strand for amplification.

Illustration showing matched antibody pair in the ProQuantum assay followed by the PCR amplification of signal that occurs when two antibodies are proimate to each other.
 Click image to enlarge

ProQuantum high-senstivity immunoassay schematic. (A) Antibody-conjugates binding to target (1-hr incubation). (B) Ligation and amplification of signal (in qPCR instrument).

Benefits of ProQuantum immunoassays kits

  • High-sensitivity—detect low levels of proteins with greater sensitivity than traditional ELISA methods.
  • Broad dynamic range—≥5 logarithmic units, minimizing sample dilutions to ensure they fall within the range.
  • Small sample consumption—use 2–5 μL of sample (instead of 75 μL for triplicate wells with other ELISA methods).
  • Fast, easy workflow—typically 2 hours from sample to answer, no wash steps, and a single 1-hour incubation.
  • No proprietary instrument to purchase—runs on any real-time PCR instrument.
  • Includes intuitive cloud-based softwarecomprehensive data analysis and statistical group–wise comparison.

How to use a ProQuantum high-sensitivity immunoassay kit

Watch this instructional video on how to run a ProQuantum assay to see the simple steps to run the assay at your lab bench.


Articles and technical guides

ELISA related products

Thermo Scientific ELISA Reagents Bottle

ELISA reagents and buffers

Wash buffers, blocking buffers and enzyme substrates.

Thermo Scientific ELISA Plate reader

ELISA instrumentation and equipment

Plate washers, plate readers, pipettes.

Thermo scientific ELISA plate formats

ELISA microplate and plasticware

Hydrophobic, hydrophilic plates, strip plates.

Additional products

Invitrogen ProcartaPlex multiplex assays kit content

ProcartaPlex multiplex assays

Multiplex immunoassays using the Luminex platform.

Invitrogen ELISA Kits custom services icon

ELISA special services

Request bulk packaging, lot reservation or custom kits.

Invitrogen Antibodies products

Antibodies

We offer a variety of specific antibodies to build your own ELISA.

Performance guarantee

If an Invitrogen immunoassay does not perform as described on our data sheet, we will replace the product at no cost to you, or if you prefer, we will provide you with a credit for future purchase.*

Learn more about performance guarantee

We're here to help

Our team of field application specialists are trained in immunoassay workflows. We can help you choose an assay or configure a panel, optimize an experiment, troubleshoot and more – face to face or on the phone at no cost.

Contact a field application specialist