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ELISA Kits
High-quality optimized Invitrogen ELISA kits allow you to measure target-specific proteins with confidence, reliability, and consistency. A variety of ELISA kit formats are available which include complete, ready-to-use kits as well as preoptimized reagents to design your own. ELISA kits and antibody pairs are available for a range of different species including human, mouse, rat, non-human primate, canine, porcine, bovine, equine, and feline. In addition to the off-the-shelf ELISA formats, special services such as: lot reservation, bulk packaging, and custom target development are available for flexibility and convenience as you start your next research project. Search all the kits available with the search bar above.
ELISA Builder makes it easy to find the best solution for developing your own ELISA assay. Based on a short list of questions about your experimental needs, the ELISA Builder tool recommends everything needed to perform an ELISA, from plates to stop solution.
Immunoassay Research Areas
Explore our immunoassays for different research areas, including immune-oncology, neurobiology, isotyping and immunoglobulin characterization, and more.
Learn more
Invitrogen ELISA formats
| Uncoated and antibody pair kits † | Coated ELISA kits* | Instant ELISA kits | ProQuantum kits | |
|---|---|---|---|---|
| Overnight coating required | Yes | No, pre-coated plate | No, pre-coated plate | No coating required |
| Analytical sensitivity** | <5 pg/ml | <5 pg/ml | <5 pg/ml | <1 pg/mL |
| Dynamic range** | <7.8–500 pg/ml | <7.8–500 pg/ml | <7.8–500 pg/ml | <1.6–5,000 pg/mL |
| Sample volume | 10–100 µL | 10–100 µL | 25–50 µL | 2–5 µL |
| Hands-on time | 1 hr 45 mins | 1 hr 20 mins | 20 mins | 30 mins |
| Incubation time** | Overnight | 2.5–4 hr | 4 hr | 1 hr |
| Instrumentation needed | Microplate reader, absorbance | Microplate reader, absorbance | Microplate reader, absorbance | qPCR instrument |
*Values in this table refer to our Standard Colorimetric kits. Ultrasensitive kits are also available.
**Every assay has its own specifications. Please consult the protocol for your specific immunoassays/kits.
Precoated ELISA kits
Invitrogen pre-coated ELISA kits are ready-to-use and have been tested to provide the lowest possible inter- and intra-assay variability and the highest possible lot-to-lot consistency. ELISA kits with precoated plates help ensure that you obtain consistent ELISA performance and are designed for labs that require validated and comprehensively tested kits.
Learn more about our ELISA kit validation and quality testing
Benefits of precoated ELISA Kits
- Validated performance—tested for plate variability (CV) and final kit validation testing before shipping out for complete confidence.
- Easy-to-use—includes all the required assay buffers and reagents, and a straightforward protocol.
- Fast—pre-coated plates allow for a workflow typically completed within 4 hours.
Included kit components
- Precoated 96-well plate
- Pretitrated capture antibody
- Pretitrated detection antibody
- Recombinant protein standard
- Stop solution
- Substrate solution
- Assay buffer (sample diluent)
- Wash buffer
Using Invitrogen pre-coated ELISA kits
View the instructional video showing how to use Invitrogen pre-coated ready-to-use ELISA kits. Please make sure to carefully read and review your ELISA kit’s specific instruction sheet, located inside the package, for detailed information before beginning the procedure.
Instant ELISA kits
Invitrogen Instant ELISA Kits have a condensed workflow format designed to alleviate the inconveniences of multiple preparation and incubation steps. In conventional ELISA kits, the precoated plates provide only the capture antibody, to which the sample and detection reagents must be added sequentially. In contrast, Instant ELISA Kits come with ready-to-use plates that contain all necessary assay components—including capture antibody and lyophilized detection antibody, streptavidin-HRP, and sample diluent. These prepared plates greatly reduce pipetting time and enable a simple one-wash protocol. Furthermore, serially diluted, lyophilized protein standards are provided in additional strip wells, eliminating the need for plate setup or serial dilutions to create a standard curve and leaving less room for error.
Click image to enlarge
Comparison of Instant ELISA technology vs. conventional ELISA procedures. With Instant ELISA kits, all the preparation work has been done for you, thereby decreasing hands-on time per experiment. In addition, Instant ELISA kits come with a full 96-well plate for your samples and two separately packed standards. This enables the use of all 96 wells for your samples expanding your ELISA capacity by 20%.
Comparison of Instant ELISA technology vs. conventional ELISA procedures. With Instant ELISA kits, all the preparation work has been done for you, thereby decreasing hands-on time per experiment. In addition, Instant ELISA kits come with a full 96-well plate for your samples and two separately packed standards. This enables the use of all 96 wells for your samples expanding your ELISA capacity by 20%.
Click image to enlarge
Fewer steps help to save time and reduce variation. There are only 7 working steps when using an Instant ELISA kit, as opposed to 17 steps when performing a conventional ELISA. Therefore, you can decrease total hands-on time per experiment to about 1 hour by adopting the Instant ELISA technique.
Fewer steps help to save time and reduce variation. There are only 7 working steps when using an Instant ELISA kit, as opposed to 17 steps when performing a conventional ELISA. Therefore, you can decrease total hands-on time per experiment to about 1 hour by adopting the Instant ELISA technique.
Instant ELISA Kits
- No preparation of reagents
- No serial dilutions of standards
- No sequential additions to the plate
Included kit components
- Precoated 96-well plate
- Pretitrated capture antibody
- Pretitrated detection antibody
- Recombinant protein standard (in additional well strips)
- Stop solution
- Substrate solution
- Assay buffer (sample diluent)
- Wash buffer
- Lysis buffer
Uncoated ELISA and antibody pair kits
Invitrogen uncoated and matched antibody pair ELISA kits have the reagents needed to coat-your-own plates, overnight process, and run the assay. Kit configurations provide easy-to-use, flexible options. Uncoated kits and antibody pair kits are ideal for experienced ELISA users on a budget with less stringent requirements for inter- and intra- assay variance.
Benefits of Uncoated ELISA kits
- Pre-titered and optimized—matched antibody pairs and all provided reagents optimized for ELISA development.
- Most options for kit configuration—purchase based on the volume of your assay through a choice of package sizes, including 2-, 10- and 20-plate kits.
- Complete—includes all the essential reagents and buffers to limit variability. Generic reagents like wash and stop solution not included.
- Affordable—prices to accommodate the most demanding budgets and to help maximize your research.
Included kit components
- Pretitrated capture antibody
- Pretitrated detection antibody
- Recombinant protein standard
- Substrate solution
- Assay buffer (sample diluent)
- Coating buffer
Benefits of antibody pair kits
- Flexible—matched antibody pairs can be used for ELISA or with other immunoassay platforms.
- Convenient packaging option—All-in-one buffer kit sold separately, if needed for ELISA development.
How to coat a plate and use Invitrogen uncoated ELISA kits
View the instructional video showing how to coat a plate and use Invitrogen uncoated ELISA kits. Please make sure to carefully read and review your ELISA kit’s specific instruction sheet, located inside the package, for detailed information before beginning the procedure.
ProQuantum Kits- high-sensitivity immunoassays
ProQuantum immunoassays utilize a matched pair of target-specific antibodies each conjugated to a DNA oligonucleotide. This platform leverages the sensitivity and large dynamic range of Applied Biosystems TaqMan real-time PCR technology. During antibody–analyte binding, the two DNA oligos are brought into close proximity, which then allows for ligation of the two strands and subsequent creation of template strand for amplification.
Click image to enlarge
ProQuantum high-sensitivity immunoassay schematic.(A) Antibody-conjugates binding to target (1-hr incubation). (B) Ligation and amplification of signal (in qPCR instrument).
Benefits of ProQuantum immunoassays kits
- High-sensitivity—detect low levels of proteins with greater sensitivity than traditional ELISA methods.
- Broad dynamic range—≥5 logarithmic units, minimizing sample dilutions to ensure they fall within the range.
- Small sample consumption—use 2–5 μL of sample (instead of 75 μL for triplicate wells with other ELISA methods).
- Fast, easy workflow—typically 2 hours from sample to answer, no wash steps, and a single 1-hour incubation.
- No proprietary instrument to purchase—runs on any real-time PCR instrument.
- Includes intuitive cloud-based software—comprehensive data analysis and statistical group–wise comparison.
How to use a ProQuantum high-sensitivity immunoassay kit
Watch this instructional video on how to run a ProQuantum assay to see the simple steps to run the assay at your lab bench.
ELISAs can be used to identify and quantitate just about any type of analyte. When designing an ELISA for your unique target of interest, deciding which components are right for you is often the most difficult part. The ELISA builder is an online selection tool that guides you through a short series of questions about your specific ELISA and then recommends all the components you’ll need—from plates to stop solution.
Invitrogen ELISA formats
| Uncoated and antibody pair kits † | Coated ELISA kits* | Instant ELISA kits | ProQuantum kits | |
|---|---|---|---|---|
| Overnight coating required | Yes | No, pre-coated plate | No, pre-coated plate | No coating required |
| Analytical sensitivity** | <5 pg/ml | <5 pg/ml | <5 pg/ml | <1 pg/mL |
| Dynamic range** | <7.8–500 pg/ml | <7.8–500 pg/ml | <7.8–500 pg/ml | <1.6–5,000 pg/mL |
| Sample volume | 10–100 µL | 10–100 µL | 25–50 µL | 2–5 µL |
| Hands-on time | 1 hr 45 mins | 1 hr 20 mins | 20 mins | 30 mins |
| Incubation time** | Overnight | 2.5–4 hr | 4 hr | 1 hr |
| Instrumentation needed | Microplate reader, absorbance | Microplate reader, absorbance | Microplate reader, absorbance | qPCR instrument |
*Values in this table refer to our Standard Colorimetric kits. Ultrasensitive kits are also available.
**Every assay has its own specifications. Please consult the protocol for your specific immunoassays/kits.
Precoated ELISA kits
Invitrogen pre-coated ELISA kits are ready-to-use and have been tested to provide the lowest possible inter- and intra-assay variability and the highest possible lot-to-lot consistency. ELISA kits with precoated plates help ensure that you obtain consistent ELISA performance and are designed for labs that require validated and comprehensively tested kits.
Learn more about our ELISA kit validation and quality testing
Benefits of precoated ELISA Kits
- Validated performance—tested for plate variability (CV) and final kit validation testing before shipping out for complete confidence.
- Easy-to-use—includes all the required assay buffers and reagents, and a straightforward protocol.
- Fast—pre-coated plates allow for a workflow typically completed within 4 hours.
Included kit components
- Precoated 96-well plate
- Pretitrated capture antibody
- Pretitrated detection antibody
- Recombinant protein standard
- Stop solution
- Substrate solution
- Assay buffer (sample diluent)
- Wash buffer
Using Invitrogen pre-coated ELISA kits
View the instructional video showing how to use Invitrogen pre-coated ready-to-use ELISA kits. Please make sure to carefully read and review your ELISA kit’s specific instruction sheet, located inside the package, for detailed information before beginning the procedure.
Instant ELISA kits
Invitrogen Instant ELISA Kits have a condensed workflow format designed to alleviate the inconveniences of multiple preparation and incubation steps. In conventional ELISA kits, the precoated plates provide only the capture antibody, to which the sample and detection reagents must be added sequentially. In contrast, Instant ELISA Kits come with ready-to-use plates that contain all necessary assay components—including capture antibody and lyophilized detection antibody, streptavidin-HRP, and sample diluent. These prepared plates greatly reduce pipetting time and enable a simple one-wash protocol. Furthermore, serially diluted, lyophilized protein standards are provided in additional strip wells, eliminating the need for plate setup or serial dilutions to create a standard curve and leaving less room for error.
Click image to enlarge
Comparison of Instant ELISA technology vs. conventional ELISA procedures. With Instant ELISA kits, all the preparation work has been done for you, thereby decreasing hands-on time per experiment. In addition, Instant ELISA kits come with a full 96-well plate for your samples and two separately packed standards. This enables the use of all 96 wells for your samples expanding your ELISA capacity by 20%.
Comparison of Instant ELISA technology vs. conventional ELISA procedures. With Instant ELISA kits, all the preparation work has been done for you, thereby decreasing hands-on time per experiment. In addition, Instant ELISA kits come with a full 96-well plate for your samples and two separately packed standards. This enables the use of all 96 wells for your samples expanding your ELISA capacity by 20%.
Click image to enlarge
Fewer steps help to save time and reduce variation. There are only 7 working steps when using an Instant ELISA kit, as opposed to 17 steps when performing a conventional ELISA. Therefore, you can decrease total hands-on time per experiment to about 1 hour by adopting the Instant ELISA technique.
Fewer steps help to save time and reduce variation. There are only 7 working steps when using an Instant ELISA kit, as opposed to 17 steps when performing a conventional ELISA. Therefore, you can decrease total hands-on time per experiment to about 1 hour by adopting the Instant ELISA technique.
Instant ELISA Kits
- No preparation of reagents
- No serial dilutions of standards
- No sequential additions to the plate
Included kit components
- Precoated 96-well plate
- Pretitrated capture antibody
- Pretitrated detection antibody
- Recombinant protein standard (in additional well strips)
- Stop solution
- Substrate solution
- Assay buffer (sample diluent)
- Wash buffer
- Lysis buffer
Uncoated ELISA and antibody pair kits
Invitrogen uncoated and matched antibody pair ELISA kits have the reagents needed to coat-your-own plates, overnight process, and run the assay. Kit configurations provide easy-to-use, flexible options. Uncoated kits and antibody pair kits are ideal for experienced ELISA users on a budget with less stringent requirements for inter- and intra- assay variance.
Benefits of Uncoated ELISA kits
- Pre-titered and optimized—matched antibody pairs and all provided reagents optimized for ELISA development.
- Most options for kit configuration—purchase based on the volume of your assay through a choice of package sizes, including 2-, 10- and 20-plate kits.
- Complete—includes all the essential reagents and buffers to limit variability. Generic reagents like wash and stop solution not included.
- Affordable—prices to accommodate the most demanding budgets and to help maximize your research.
Included kit components
- Pretitrated capture antibody
- Pretitrated detection antibody
- Recombinant protein standard
- Substrate solution
- Assay buffer (sample diluent)
- Coating buffer
Benefits of antibody pair kits
- Flexible—matched antibody pairs can be used for ELISA or with other immunoassay platforms.
- Convenient packaging option—All-in-one buffer kit sold separately, if needed for ELISA development.
How to coat a plate and use Invitrogen uncoated ELISA kits
View the instructional video showing how to coat a plate and use Invitrogen uncoated ELISA kits. Please make sure to carefully read and review your ELISA kit’s specific instruction sheet, located inside the package, for detailed information before beginning the procedure.
ProQuantum Kits- high-sensitivity immunoassays
ProQuantum immunoassays utilize a matched pair of target-specific antibodies each conjugated to a DNA oligonucleotide. This platform leverages the sensitivity and large dynamic range of Applied Biosystems TaqMan real-time PCR technology. During antibody–analyte binding, the two DNA oligos are brought into close proximity, which then allows for ligation of the two strands and subsequent creation of template strand for amplification.
Click image to enlarge
ProQuantum high-sensitivity immunoassay schematic.(A) Antibody-conjugates binding to target (1-hr incubation). (B) Ligation and amplification of signal (in qPCR instrument).
Benefits of ProQuantum immunoassays kits
- High-sensitivity—detect low levels of proteins with greater sensitivity than traditional ELISA methods.
- Broad dynamic range—≥5 logarithmic units, minimizing sample dilutions to ensure they fall within the range.
- Small sample consumption—use 2–5 μL of sample (instead of 75 μL for triplicate wells with other ELISA methods).
- Fast, easy workflow—typically 2 hours from sample to answer, no wash steps, and a single 1-hour incubation.
- No proprietary instrument to purchase—runs on any real-time PCR instrument.
- Includes intuitive cloud-based software—comprehensive data analysis and statistical group–wise comparison.
How to use a ProQuantum high-sensitivity immunoassay kit
Watch this instructional video on how to run a ProQuantum assay to see the simple steps to run the assay at your lab bench.
ELISAs can be used to identify and quantitate just about any type of analyte. When designing an ELISA for your unique target of interest, deciding which components are right for you is often the most difficult part. The ELISA builder is an online selection tool that guides you through a short series of questions about your specific ELISA and then recommends all the components you’ll need—from plates to stop solution.
Articles and technical guides
Easier ELISA Workflows
Learn how Invitrogen Instant ELISA kits can streamline your ELISA workflow.
Time-saving immunoassay tools
Find the right immunoassay for your research needs.
Biomarker quantitation assay guide
Immunoassay guide to protein quantitation.
ELISA related products
ELISA reagents and buffers
Wash buffers, blocking buffers and enzyme substrates.
ELISA instrumentation and equipment
Plate washers, plate readers, pipettes.
ELISA microplate and plasticware
Hydrophobic, hydrophilic plates, strip plates.
Additional products
ProcartaPlex multiplex assays
Multiplex immunoassays using the Luminex platform.
ELISA special services
Request bulk packaging, lot reservation or custom kits.
Antibodies
We offer a variety of specific antibodies to build your own ELISA.
Performance guarantee
If an Invitrogen immunoassay does not perform as described on our data sheet, we will replace the product at no cost to you, or if you prefer, we will provide you with a credit for future purchase.*
Learn more about performance guarantee
We're here to help
Our team of field application specialists are trained in immunoassay workflows. We can help you choose an assay or configure a panel, optimize an experiment, troubleshoot and more – face to face or on the phone at no cost.
Contact a field application specialist