Invitrogen pre-coated ELISA kits are ready-to-use and have been tested to provide the lowest possible inter- and intra-assay variability and the highest possible lot-to-lot consistency. ELISA kits with precoated plates help ensure that you obtain consistent ELISA performance and are designed for labs that require validated and comprehensively tested kits.

Learn more about our ELISA kit validation and quality testing

Benefits of precoated ELISA Kits

• Validated performance⁠—tested for plate variability (CV) and final kit validation testing before shipping out for complete confidence.
• Easy-to-use⁠—includes all the required assay buffers and reagents, and a straightforward protocol.
• Fast⁠—pre-coated plates allow for a workflow typically completed within 4 hours.

Invitrogen uncoated and matched antibody pair ELISA kits have the reagents needed to coat-your-own plates, overnight process, and run the assay. Kit configurations provide easy-to-use, flexible options. Uncoated kits and antibody pair kits are ideal for experienced ELISA users on a budget with less stringent requirements for inter- and intra- assay variance.

Benefits of Uncoated ELISA kits

• Pre-titered and optimized—matched antibody pairs and all provided reagents optimized for ELISA development.
• Most options for kit configuration—purchase based on the volume of your assay through a choice of package sizes, including 2-, 10- and 20-plate kits.
• Complete—includes all the essential reagents and buffers to limit variability. Generic reagents like wash and stop solution not included.
• Affordable—prices to accommodate the most demanding budgets and to help maximize your research.

Benefits of antibody pair kits

• Flexible—matched antibody pairs can be used for ELISA or with other immunoassay platforms.
• Convenient packaging option—All-in-one buffer kit sold separately, if needed for ELISA development.

Invitrogen Instant ELISA Kits have a condensed workflow format designed to alleviate the inconveniences of multiple preparation and incubation steps. In conventional ELISA kits, the precoated plates provide only the capture antibody, to which the sample and detection reagents must be added sequentially. In contrast, Instant ELISA Kits come with ready-to-use plates that contain all necessary assay components—including capture antibody and lyophilized detection antibody, streptavidin-HRP, and sample diluent. These prepared plates greatly reduce pipetting time and enable a simple one-wash protocol. Furthermore, serially diluted, lyophilized protein standards are provided in additional strip wells, eliminating the need for plate setup or serial dilutions to create a standard curve and leaving less room for error.

ProQuantum immunoassays utilize a matched pair of target-specific antibodies each conjugated to a DNA oligonucleotide. This platform leverages the sensitivity and large dynamic range of Applied Biosystems TaqMan real-time PCR technology. During antibody–analyte binding, the two DNA oligos are brought into close proximity, which then allows for ligation of the two strands and subsequent creation of template strand for amplification.

Benefits of ProQuantum immunoassays kits

• High-sensitivity—detect low levels of proteins with greater sensitivity than traditional ELISA methods.
• Broad dynamic range—≥5 logarithmic units, minimizing sample dilutions to ensure they fall within the range.
• Small sample consumption—use 2–5 μL of sample (instead of 75 μL for triplicate wells with other ELISA methods).
• Fast, easy workflow—typically 2 hours from sample to answer, no wash steps, and a single 1-hour incubation.
• No proprietary instrument to purchase—runs on any real-time PCR instrument.
• Includes intuitive cloud-based software—comprehensive data analysis and statistical group–wise comparison.

Learn more about ProQuantum immunoassay kits