ELISA Kits and ELISA Components
High-quality, optimized kits and reagents for accurate
High-quality optimized Invitrogen ELISA kits can help you measure target-specific proteins with confidence, reliability, and consistency. A variety of ELISA kit formats are available which include complete, ready-to-use kits as well as preoptimized reagents to design your own assay. ELISA kits and antibody pairs are available for a range of different species including human, mouse, rat, non-human primate, canine, porcine, bovine, equine, and feline. In addition to the off-the-shelf ELISA formats, special services such as: lot reservation, bulk packaging, and custom target development are available for flexibility and convenience as you start your next research project.
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|Coated ELISA kits*||Instant ELISA kits||Uncoated ELISA kits||Matched Antibody Pair kits||PeproTech ELISA Development Kits|
|Explore coated ELISA targets||Explore Instant ELISA targets||Explore uncoated ELISA targets||Explore matched antibody pair targets||Explore ELISA development kit targets|
|Overnight coating||Not required||Not required||Required||Required||Required|
|Analytical sensitivity**||Kit specific||5 pg/mL||Lot specific|
|Dynamic range**||2—3 logarithmic units||pg/mL (lot specific)|
|Sample volume||5–100 µL|
(sample type specific)
(sample type specific)
|Sample-to-result time||45—300 min|
|24 hr||>24 hr||>24 hr|
|Learn more below about coated ELISA kits||Learn more below about Instant ELISA kits||Learn more below about uncoated ELISA kits||Learn more about matched antibody pair kits||Learn more below about ELISA development kits|
*Values in this table refer to our Standard Colorimetric kits. A select number of ultrasensitive kits are also available.
**Every assay has its own specifications. Please consult the protocol for your specific immunoassays/kits.
Utilizing proximity-based amplification technology, ProQuantum assays offer the analyte specificity of antibody-antigen binding with the signal detection and amplification capabilities of qPCR to achieve a highly sensitive assay.
ELISA Builder can make it easy to find a great solution for developing your own ELISA assay. Based on a short list of questions about your experimental needs, the ELISA Builder tool recommends everything needed to perform an ELISA, from plates to stop solution.
Invitrogen coated ELISA kits are ready-to-use, and have been tested to provide very low inter- and intra-assay variability, with very high lot-to-lot consistency. ELISA kits with coated plates help ensure that you obtain consistent ELISA performance and are designed for labs that require validated and comprehensively tested kits.
View the instructional video showing how to use Invitrogen pre-coated ready-to-use ELISA kits. Please make sure to carefully read and review your ELISA kit’s specific instruction sheet, located inside the package, for detailed information before beginning the procedure.
Invitrogen Instant ELISA Kits have a condensed workflow format designed to alleviate the inconveniences of multiple preparation and incubation steps. In conventional ELISA kits, the precoated plates provide only the capture antibody, to which the sample and detection reagents must be added sequentially. In contrast, Instant ELISA Kits come with ready-to-use plates that contain all necessary assay components—including capture antibody and lyophilized detection antibody, streptavidin-HRP, and sample diluent. These prepared plates greatly reduce pipetting time and enable a simple one-wash protocol. Furthermore, serially diluted, lyophilized protein standards are provided in additional strip wells, eliminating the need for plate setup and serial dilutions to create a standard curve, leaving less room for error.
Figure 1. Comparison of Instant ELISA technology vs. conventional ELISA procedures. With Instant ELISA kits, all the preparation work has been done for you, helping decrease hands-on time per experiment. In addition, Instant ELISA kits come with a full 96-well plate for your samples and two separately packed standards. This enables the use of all 96 wells for your samples expanding your ELISA capacity by 20%.
Table 1. Comparing conventional ELISA and Instant ELISA workflow steps. Fewer steps help save time and reduce variation. There are only 7 working steps when using an Instant ELISA kit, as opposed to 17 steps when performing a conventional ELISA. Therefore, you can decrease total hands-on time per experiment to about 1 hour by adopting the Instant ELISA technique.
|7 steps for Instant ELISA kits||17 steps for conventional ELISA kits|
|1. Washing of coated plates|
2. Reconstitution of standard protein
3. Addition of diluent to standard wells
|1. Rehydration of standard and sample wells on plate||4.Titration of standard curve|
5. Addition of sample diluent
|2. Sample addition||6. Sample addition|
|7. Dilution of biotin conjugate|
8. Addition of biotin conjugate
|3. Incubation||9. Incubation|
|10. Preparation of streptavidin-HRP conjugate|
12. Addition of streptavidin-HRP conjugate
|4. Washing||14. Washing|
|5. Addition of TMB substrate||15. Addition of TMB substrate|
|6. Addition of stop solution||16. Addition of stop solution|
|7. Calculation of results||16. Calculation of results|
ELISAs can be used to identify and quantitate just about any type of analyte. When designing an ELISA for your unique target of interest, deciding which components are right for you is often the most difficult part. The ELISA Builder is an online selection tool that guides you through a short series of questions about your specific ELISA and then recommends all the components you’ll need—from plates to stop solution.
Invitrogen uncoated kits are ideal for experienced ELISA users on a budget with less stringent requirements for inter- and intra- assay variance. These kits have the reagents needed to coat-your-own plates using an overnight process and run the assay. Kit configurations can provide easy-to-use, flexible options.
Invitrogen antibody pair kits contain pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own ELISA assays. PeproTech Development kits are also used to design new ELISA protocols, with a variety of targets available in both ABTS and TMB formats for flexibility.
View the instructional video showing how to coat a plate and use Invitrogen uncoated ELISA kits. Please make sure to carefully read and review your ELISA kit’s specific instruction sheet, located inside the package, for detailed information before beginning the procedure.
For Research Use Only. Not for use in diagnostic procedures.