Profile more biomarkers with less sample

Invitrogen ProcartaPlex immunoassays are antibody-based, magnetic bead reagent kits and panels for multiplex protein quantitation using the Luminex instrument platform. The assays cover more than 300 cytokines, chemokines, growth factors, and other protein targets from human, mouse, rat, non-human primate, canine, and other species. Select from 90+ preconfigured multiplex panels (2- to 65-plex), or blend singleplex kits to create custom multiplex assays.

View ProcartaPlex Cytokine Storm Biomarker Panel

ProcartaPlex multiplex assay options

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Preconfigured panels

Mix & Match panels

Simplex Kits

Predefined, biologically relevant, disease-defined panels using magnetic beads for quantitative multiplex analysis of up to 65 analytes in a single sample. Optimal performance and reproducibility through extensive testing for combinability, cross-reactivity, and interference.

Search available preconfigured panels

Custom-blended panels deliver results tailored to your choice of targets. Simply provide your desired species, sample type, and instrument for use. Then select from our list of analytes and we will formulate a user-defined panel that is supplied with all the necessary reagents to perform the assay.

Build your own panel

Bead sets for the detection of individual analytes designed to build your own multiplex panel from our catalog of assays. Multiple Simplex kits can be combined and run using the ProcartaPlex Basic kit containing all non–target-specific reagents needed to perform the assay.

Search available Simplex Kits


ProcartaPlex multiplex immunoassay kits

Invitrogen ProcartaPlex multiplex immunoassays make use of Luminex xMAP (multianalyte profiling) technology to enable the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 µL sample of plasma, serum, cell culture supernatants, and other bodily fluids. Luminex technology uses differentially dyed capture beads for each target in a multiplex ELISA like assay. ProcartaPlex immunoassays enable the simultaneous analysis of multiple proteins in a single sample from a broad range of biological sources. They combine the efficiencies of multiplexing with the accuracy, sensitivity, reproducibility, and simplicity of ELISA.

Key features of ProcartaPlex immunoassays

  • Multianalyte detection with a large dynamic range
  • All ProcartaPlex assays are individually tested for specificity and endogenous, native protein detection
  • ProcartaPlex Preconfigured panels are extensively tested for combinability, interference, and cross-reactivity
  • Scalable and reproducible performance regardless of plex size
  • Magnetic bead format for reliable washing
  • Largest published panel for quantitation of up to 65 analytes
     

ProcartaPlex workflow

ProcartaPlex multiplex assay kits are performed in much the same way as ELISAs. The steps are similar, with the exception that antibody-coated beads are used to capture the analyte, instead of having capture antibodies attached directly to the plate. Each kit provides standards of known concentration so that a standard curve can be established. After incubation on a shaker, the beads are washed by putting the 96-well plate on a flat magnet for 30 seconds, after which the fluid is discarded by flicking the wells or by using an automated plate washer. The magnet is removed, and the beads are resuspended in the detection antibody. Another incubation and wash are followed by the addition of streptavidin–R-phycoerythrin (SAPE). The beads are then washed and are ready to analyze.

ProcartaPlex Multiplex Immunoassays for the Luminex platform are performed in a similar way as traditional ELISAs but with the ability to quantitate up to 65 targets from a single sample.

Invitrogen ProcartaPlex Multiplex Immunoassay protocol general workflow

Figure 1. General workflow for the Invitrogen ProcartaPlex Multiplex Immunoassay.
 

Over 500 analytes are available for human, mouse, rat, non-human primate (NHP), canine, and porcine samples. Below are analytes that are available either in preconfigured multiplex panels or available as Simplex Kits for building your own assay.

View full listing of preconfigured panelsView full listing of Simplex Kits


View analytes by name


Analyte A_B


Analyte C


Analytes D_F


Analytes G_H


Analytes IC_IG
Analytes IL-IT


Analytes K_L


Analytes M


Analytes N_P
*Analytes only available as part of a panel.


Analytes R_S


Analytes T
*Analytes only available as part of a panel.


Analytes U_Z

Design your own assays

ProcartaPlex assays provide the ultimate flexibility; you can select your own Simplex assays and mix them yourself, or design the panel of your choice with our online Invitrogen ProcartaPlex Panel Configurator. Select your analytes of interest, and immediately receive recommendations for the most effective solutions for your panel needs. Each customized panel is formulated and supplied with all the necessary reagents to perform the assay, including premixed capture beads, premixed detection reagents, protein standards, and buffers. If you are not sure which solution is right for you, use the panel configurator to discover custom and catalog options for your targets of interest.

Use the following tool to select analytes of interest and build your panel:

ProcartaPlex Panel Configurator and Selection Tool

ProcartaPlex multiplex immunoassay kits

Invitrogen ProcartaPlex multiplex immunoassays make use of Luminex xMAP (multianalyte profiling) technology to enable the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 µL sample of plasma, serum, cell culture supernatants, and other bodily fluids. Luminex technology uses differentially dyed capture beads for each target in a multiplex ELISA like assay. ProcartaPlex immunoassays enable the simultaneous analysis of multiple proteins in a single sample from a broad range of biological sources. They combine the efficiencies of multiplexing with the accuracy, sensitivity, reproducibility, and simplicity of ELISA.

Key features of ProcartaPlex immunoassays

  • Multianalyte detection with a large dynamic range
  • All ProcartaPlex assays are individually tested for specificity and endogenous, native protein detection
  • ProcartaPlex Preconfigured panels are extensively tested for combinability, interference, and cross-reactivity
  • Scalable and reproducible performance regardless of plex size
  • Magnetic bead format for reliable washing
  • Largest published panel for quantitation of up to 65 analytes
     

ProcartaPlex workflow

ProcartaPlex multiplex assay kits are performed in much the same way as ELISAs. The steps are similar, with the exception that antibody-coated beads are used to capture the analyte, instead of having capture antibodies attached directly to the plate. Each kit provides standards of known concentration so that a standard curve can be established. After incubation on a shaker, the beads are washed by putting the 96-well plate on a flat magnet for 30 seconds, after which the fluid is discarded by flicking the wells or by using an automated plate washer. The magnet is removed, and the beads are resuspended in the detection antibody. Another incubation and wash are followed by the addition of streptavidin–R-phycoerythrin (SAPE). The beads are then washed and are ready to analyze.

ProcartaPlex Multiplex Immunoassays for the Luminex platform are performed in a similar way as traditional ELISAs but with the ability to quantitate up to 65 targets from a single sample.

Invitrogen ProcartaPlex Multiplex Immunoassay protocol general workflow

Figure 1. General workflow for the Invitrogen ProcartaPlex Multiplex Immunoassay.
 

Over 500 analytes are available for human, mouse, rat, non-human primate (NHP), canine, and porcine samples. Below are analytes that are available either in preconfigured multiplex panels or available as Simplex Kits for building your own assay.

View full listing of preconfigured panelsView full listing of Simplex Kits


View analytes by name


Analyte A_B


Analyte C


Analytes D_F


Analytes G_H


Analytes IC_IG
Analytes IL-IT


Analytes K_L


Analytes M


Analytes N_P
*Analytes only available as part of a panel.


Analytes R_S


Analytes T
*Analytes only available as part of a panel.


Analytes U_Z

Design your own assays

ProcartaPlex assays provide the ultimate flexibility; you can select your own Simplex assays and mix them yourself, or design the panel of your choice with our online Invitrogen ProcartaPlex Panel Configurator. Select your analytes of interest, and immediately receive recommendations for the most effective solutions for your panel needs. Each customized panel is formulated and supplied with all the necessary reagents to perform the assay, including premixed capture beads, premixed detection reagents, protein standards, and buffers. If you are not sure which solution is right for you, use the panel configurator to discover custom and catalog options for your targets of interest.

Use the following tool to select analytes of interest and build your panel:

ProcartaPlex Panel Configurator and Selection Tool

Related products and resources

Luminex assays and equipment

Single analyte assays

Performance guarantee

If an Invitrogen immunoassay does not perform as described on our data sheet, we will replace the product at no cost to you, or if you prefer, we will provide you with a credit for future purchase.*

Learn more about performance guarantee

We're here to help

Our team of field application specialists are trained in immunoassay workflows. We can help you choose an assay or configure a panel, optimize an experiment, troubleshoot and more – face to face or on the phone at no cost.

Contact a field application specialist