Profile more biomarkers with less sample

Invitrogen ProcartaPlex immunoassays are antibody-based, magnetic bead reagent kits and panels for multiplex protein quantitation using the Luminex instrument platform. The assays cover more than 600 cytokines, chemokines, growth factors, and other protein targets from human, mouse, rat, non-human primate, canine, and other species. Select from 90+ preconfigured multiplex panels (2- to 80-plex), or blend singleplex kits to create custom multiplex assays.

New SARS-CoV-2 Serology Assays

Rapidly detect antibodies (immunoglobulins, IgG and IgM) in serum against the SARS-CoV-2 coronavirus.

Design your ProcartaPlex panel

Design your own multiplex immunoassay for protein detection using a Luminex instrument. 

Inflammation and cytokine storm assays

Measure biomarkers of cytokine storm and inflammation.


ProcartaPlex multiplex assay options


About ProcartaPlex assays

ProcartaPlex multiplex immunoassay kits

ProcartaPlex multiplex immunoassays make use of Luminex xMAP (multi-analyte profiling) technology to enable the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample of plasma, serum, cell culture supernatants, and other bodily fluids. Luminex technology uses differentially dyed capture beads for each target in a multiplex ELISA like assay. ProcartaPlex immunoassays enable the simultaneous analysis of multiple proteins in a single sample from a broad range of biological sources. They combine the efficiencies of multiplexing with the accuracy, sensitivity, reproducibility, and simplicity of ELISA.

 

Key features of ProcartaPlex immunoassays:

  • Multi-analyte detection with a large dynamic range
  • All ProcartaPlex assays are individually tested for specificity and detection of native endogenous protein
  • ProcartaPlex Preconfigured panels are extensively tested for combinability, interference, and cross-reactivity
  • Scalable and reproducible performance regardless of plex size
  • Magnetic bead format for reliable washing
  • Largest published panel for quantitation of up to 65 analyte proteins
  • New 384-well format with lyophilized beads options

ProcartaPlex workflow

ProcartaPlex multiplex assay kits are performed in much the same way as ELISAs. The steps are similar, with the exception that antibody-coated beads are used to capture the analyte, instead of having capture antibodies attached directly to the plate. Each kit provides standards of known concentration so that a standard curve can be established. After incubation on a shaker, the beads are washed by putting the 96-well plate on a flat magnet for 30 seconds, after which the fluid is discarded by flicking the wells or by using an automated plate washer. The magnet is removed, and the beads are resuspended in the detection antibody. Another incubation and wash are followed by the addition of streptavidin–R-phycoerythrin (SAPE). The beads are then washed and are ready to analyze. Access streamlined analysis with the ProcartaPlex Analysis App.

ProcartaPlex multiplex immunoassays for the Luminex platform are performed in a similar way as traditional ELISAs but with the ability to quantitate up to 65 targets from a single sample.

9-step workflow diagram for ProcartaPlex Immunoassays
General workflow for the Invitrogen ProcartaPlex multiplex immunoassay

Over 600 analytes are available for human, mouse, rat, non-human primate (NHP), canine, and porcine samples. Below are analytes that are available either in preconfigured multiplex panels or available as Simplex Kits for building your own assay. Note that analytes in the tables below marked with * are only available as part of a panel.

Design your own assays

ProcartaPlex assays offer the ultimate flexibility; you can select your own Simplex assays and mix them yourself, or design the panel of your choice with our online Invitrogen ProcartaPlex Panel Configurator. Select your analytes of interest, and immediately receive recommendations for the most effective solutions for your panel needs. Each customized panel is formulated and supplied with all the necessary reagents to perform the assay, including premixed capture beads, premixed detection reagents, protein standards, and buffers. If you are not sure which solution is right for you, use the panel configurator to discover custom and catalog options for your targets of interest.

 

Use the ProcartaPlex Panel Configurator and Selection Tool to select analytes of interest and build your panel.

Immunoassay research areas

Explore our immunoassays for different research areas, including immune-oncology, neurobiology, isotyping and immunoglobulin characterization, and more.

Immunoassay research areas

ProQuantum High Sensitivity Immunoassays

Detect very low levels of proteins using as little as 2–5 μL of sample.

ProQuantum High Sensitivity Immunoassays

QuantiGene Plex gene expression assays

Multiplex up to 80 RNA targets from a single biological sample.

QuantiGene Plex gene expression assays

Invitrogen ELISA Kits

Quantitate single analytes with confidence with highly verified Invitrogen ELISA kits.

Invitrogen ELISA Kits

For Research Use Only. Not for use in diagnostic procedures.