Invitrogen ProcartaPlex immunoassays are antibody-based, magnetic bead reagent kits and panels for multiplex protein quantitation using the Luminex instrument platform. The assays cover more than 600 cytokines, chemokines, growth factors, and other protein targets from human, mouse, rat, non-human primate, canine, and other species. Select from 90+ preconfigured multiplex panels (2- to 80-plex), or blend singleplex kits to create custom multiplex assays.
|Preconfigured panels||Mix & Match||Simplex Kits|
Predefined, biologically relevant, disease-defined panels using magnetic beads for quantitative multiplex analysis of up to 80 analytes in a single sample. Optimal performance and reproducibility through extensive testing for combinability, cross-reactivity, and interference.
Custom-blended panels deliver results tailored to your choice of targets. Simply provide your desired species, sample type, and instrument for use. Then select from our list of analytes and we will formulate a user-defined panel that is supplied with all the necessary reagents to perform the assay.
Bead sets for the detection of individual analytes designed to build your own multiplex panel from our catalog of assays. Multiple Simplex kits can be combined and run using the ProcartaPlex Basic kit containing all non–target-specific reagents needed to perform the assay.
|Search available preconfigured panels||Build your own panel||Search available Simplex Kits|
ProcartaPlex multiplex immunoassays make use of Luminex xMAP (multi-analyte profiling) technology to enable the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample of plasma, serum, cell culture supernatants, and other bodily ﬂuids. Luminex technology uses differentially dyed capture beads for each target in a multiplex ELISA like assay. ProcartaPlex immunoassays enable the simultaneous analysis of multiple proteins in a single sample from a broad range of biological sources. They combine the efficiencies of multiplexing with the accuracy, sensitivity, reproducibility, and simplicity of ELISA.
Key features of ProcartaPlex immunoassays:
ProcartaPlex multiplex assay kits are performed in much the same way as ELISAs. The steps are similar, with the exception that antibody-coated beads are used to capture the analyte, instead of having capture antibodies attached directly to the plate. Each kit provides standards of known concentration so that a standard curve can be established. After incubation on a shaker, the beads are washed by putting the 96-well plate on a flat magnet for 30 seconds, after which the fluid is discarded by flicking the wells or by using an automated plate washer. The magnet is removed, and the beads are resuspended in the detection antibody. Another incubation and wash are followed by the addition of streptavidin–R-phycoerythrin (SAPE). The beads are then washed and are ready to analyze. Access streamlined analysis with the ProcartaPlex Analysis App.
ProcartaPlex multiplex immunoassays for the Luminex platform are performed in a similar way as traditional ELISAs but with the ability to quantitate up to 80 targets from a single sample.
Over 600 analytes are available for human, mouse, rat, non-human primate (NHP), canine, and porcine samples. Below are analytes that are available either in preconfigured multiplex panels or available as Simplex Kits for building your own assay. Note that analytes in the tables below marked with * are only available as part of a panel.
ProcartaPlex assays offer the ultimate flexibility; you can select your own Simplex assays and mix them yourself, or design the panel of your choice with our online Invitrogen ProcartaPlex Panel Configurator. Select your analytes of interest, and immediately receive recommendations for the most effective solutions for your panel needs. Each customized panel is formulated and supplied with all the necessary reagents to perform the assay, including premixed capture beads, premixed detection reagents, protein standards, and buffers. If you are not sure which solution is right for you, use the panel configurator to discover custom and catalog options for your targets of interest.
Use the ProcartaPlex Panel Configurator and Selection Tool to select analytes of interest and build your panel.
For Research Use Only. Not for use in diagnostic procedures.