ProcartaPlex Immunoassays

ProcartaPlex multiplex immunoassays make use of Luminex xMAP (multi-analyte profiling) technology to enable the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 µL sample of plasma, serum, cell culture supernatants, and other bodily fluids. Luminex technology uses differentially dyed capture beads for each target in a multiplex ELISA like assay. ProcartaPlex immunoassays enable the simultaneous analysis of multiple proteins in a single sample from a broad range of biological sources. They combine the efficiencies of multiplexing with the accuracy, sensitivity, reproducibility, and simplicity of ELISA.

Key features of ProcartaPlex immunoassays:

• Multi-analyte detection with a large dynamic range
• All ProcartaPlex assays are individually tested for specificity and detection of native endogenous protein
• ProcartaPlex Preconfigured panels are extensively tested for combinability, interference, and cross-reactivity
• Scalable and reproducible performance regardless of plex size
• Magnetic bead format for reliable washing
• Largest published panel for quantitation of up to 65 analyte proteins
• New 384-well format with lyophilized beads options

ProcartaPlex multiplex assay kits are performed in much the same way as ELISAs. The steps are similar, with the exception that antibody-coated beads are used to capture the analyte, instead of having capture antibodies attached directly to the plate. Each kit provides standards of known concentration so that a standard curve can be established. After incubation on a shaker, the beads are washed by putting the 96-well plate on a flat magnet for 30 seconds, after which the fluid is discarded by flicking the wells or by using an automated plate washer. The magnet is removed, and the beads are resuspended in the detection antibody. Another incubation and wash are followed by the addition of streptavidin–R-phycoerythrin (SAPE). The beads are then washed and are ready to analyze. Access streamlined analysis with the ProcartaPlex Analysis App.

ProcartaPlex multiplex immunoassays for the Luminex platform are performed in a similar way as traditional ELISAs but with the ability to quantitate up to 80 targets from a single sample.