The ProcartaPlex 384-well assays enable multiplex protein analysis in a high throughput format with optimized reagent formulation specifically developed for smaller reaction volumes in 384-well flat bottom plates that require less sample input compared to the 96-well format. This high throughput, magnetic bead-based immunoassay allows for testing of up to 65 analytes per well and can be read on Luminex xMAP instruments—FLEXMAP 3D and INTELLIFLEX.
|Linearity||4–5 logs||4–5 logs|
|Plex level||up to 65||up to 65|
|Sample input||Serum, Plasma, CCS, CSF||Serum, Plasma, CCS, CSF|
|Compatible Luminex instruments||MAGPIX, Luminex 200, FLEXMAP 3D, INTELLIFLEX||FLEXMAP 3D, INTELLIFLEX|
|Sample volume||25 µl||6.3 µl|
Table 1. Comparison of 96-well and 384-well ProcartaPlex Assays.
We offer a unique solution with pre-dispensed, lyophilized beads on 384-well flat bottom plates available for Human and Mouse. This convenient solution offers less hands-on time, less time-to-result, and seamless integration into workflows compared to other Luminex multiplex immunoassay offerings.
Customize your panel using the ProcartaPlex Panel Configurator below and select "Lyophilized on plate" for bead formulation or select one of our preconfigured lyophilized panels.
Figure 1. The Invitrogen ProcartaPlex 384-well Multiplex Immunoassay with lyophilized beads eliminates the bead dispensation step in the general workflow minimizing bead dispensation errors.
Choose from the largest selection of analytes available in 384-well format for Human, Mouse, Rat, Canine, and NHP. Premixed bead sets are provided as classical liquid formulation provided in a single vial to be pipetted on 384-well flat bottom plates.
Create your unique ProcartaPlex Mix & Match Panel using the ProcartaPlex Panel Configurator below and select “Liquid” for bead formulation.
Figure 2. General workflow for Invitrogen ProcartaPlex 384-well Multiplex Immunoassay with liquid bead option.
All ProcartaPlex assays are extensively tested for specificity, sensitivity, and precision to ensure optimal performance. ProcartaPlex 384-well format helps to increase sample throughput while maintaining the robustness of a 96-well assay.
The following data will highlight the differences between the 96-well and 384-well protocols and compare performance data generated using a FLEXMAP3D instrument operated with increased signal enhancing by the photomultiplier tube and an automated washer.
The ProcartaPlex 384-well format provides increased sample throughput with comparable sensitivity to the 96-well format.
|Analyte||Assay range (pg/ml)||96-well||384-well (liquid)||384-well (lyophilized)|
|GRO alpha (CXCL1)||12500—3.1||1.2||0.9||0.7|
|MIP-1 alpha (CCL3)||15100—3.7||0.5||0.4||0.3|
|MIP-1 beta (CCL4)||47400—11.6||1.0||0.8||0.7|
Table 2. Comparison of sensitivities of analytes from the same standard mix lot in different product formats.
The graphs below compare 7-point standard curves for selected analytes in 96-well and 384-well formats. Similar standard curve performances in 96-well and 384-well formats ensure consistent results regardless of ProcartaPlex assay format.
A comparison of ProcartaPlex 96-well and 384-well sample values measured on a FLEXMAP 3D instrument for selected analytes is illustrated in the graphs below. Measured sample concentrations for 16 human plasma samples are shown.
The high correlation of sample concentrations across the ProcartaPlex formats ensure reliable and reproducible results.
The ProcartaPlex 384-well format was specifically developed to increase sample throughput while maintaining the robustness of a 96-well assay. Data of selected analytes in the graphs below illustrate sample correlations between 96-well and 384-well format of 16 human plasma samples.
High sample correlation coefficients (R2) make it easy to switch between 96-well and 384-well formats with consistent results.
ProcartaPlex 384-well assays are available in two formats: ready-to-use plates with pre-dispensed lyophilized beads for workflow convenience (384-well lyophilized) and as liquid formulation of bead sets in a vial to be pipetted onto the 384-well flat bottom plate (384-well liquid).
Data of selected analytes in the graphs below illustrate sample correlations between the 384-well lyophilized and 384-well liquid format obtained for 16 human plasma samples.
The high sample coefficients (R2) clearly demonstrate identical performance of ProcartaPlex 384-well assays with lyophilized and liquid bead formulation.