Add targets to multiplex kits or produce custom kits
ProcartaPlex Simplex kits are magnetic bead sets used to detect individual analytes. They are designed to enable the addition of targets to multiplex panels or the combination of individual simplex kits to produce custom panels. A corresponding basic kit is required when combining simplex kits separate from any multiplex panel.
The following table describes the level of quality standards developed and required for each ProcartaPlex Simplex Kit. Additionally, these Simplex kits are tested for combinability with other Simplex kits.
Quality control standards for ProcartaPlex Simplex Kits
|Specificity||Prove of endogenous, native protein detection in specific samples|
|Sensitivity||Detection of relevant native protein levels|
|Precision||Inter-assay CV: <15%, Intra-assay CV: <15%|
|Linearity of Dilution||Linear results over the range of the assay|
|Flexibility||More than 90% of ProcartaPlex targets can be combined with one another|
|Lot-to-lot consistency||Each new batch is calibrated against the reference standard lot|
|Dynamic range||Large dynamic range of all ProcartaPlex assays|
Example data generated in the development process for a ProcartaPlex Simplex kit
|PD-L2 in standard ProcartaPlex format||Serum||EDTA Plasma||Citrate Plasma||Heparin Plasma|
|No. of Samples||40||40||40||40|
|No. of positive samples||40||40||40||40|
Sensitivity: Detection of relevant native protein levels for typical sample types.
|Analyte||Sera of healthy Donors||Sera of Cancer Patients|
|mean pg/ml (n=16)||mean pg/ml (n=16)|
Specificity: Proof of endogenous levels of native protein detection in specific samples-healthy vs disease.
ProcartaPlex basic kits
To run simplex kits alone or in combination, the corresponding sample-specific basic kit is required. These basic kits contain all necessary support reagents to perform the assay.
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