A primary antibody is an immunoglobulin that specifically binds to a particular protein or other biomolecule of research interest for the purpose of purifying or detecting and measuring it. Primary antibodies are developed as polyclonal or monoclonal antibodies using mouse, rat, rabbit, goat, and other animal species as hosts. They are produced and supplied in various forms, ranging from crude antiserum to antigen-purified preparations. Primary antibodies for frequently researched targets are also available conjugated to fluorescent dyes or biotin.
The Invitrogen antibody portfolio is expanding—see the latest additions
We are continuously updating our Invitrogen primary antibody portfolio with advanced verified products for use in flow cytometry, IHC/IF/ICC, western blotting, ELISA, and other applications. Checkout our latest antibody highlights:
TNFAIP3 antibody for research in inflammatory disorders and tumor progression
A20, or TNF-α–induced protein 3 (TNFAIP3), is a protein that inhibits apoptosis and is a negative regulator of the NF-κB pathway through its dual function as a deubiquitinase and ubiquitin ligase, thereby preventing deleterious effects of uncontrolled inflammation. It is upregulated in response to proinflammatory cytokines like TNF-α and IL-1 and phorbol esters like PMA. Overexpression of TNFAIP3 has also been found in breast tumors, pancreatic cancer, hepatocellular carcinoma, and bladder cancer.
The Invitrogen TNFAIP3 monoclonal antibody has been tested in western blotting. Upregulation of TNFAIP3 was observed in cells upon treatment with proinflammatory agents, such as TNF-α, IL-1α, and PMA.
TNFAIP3 monoclonal antibody (Cat. No. MA5-16164) shows upregulation of TNFAIP3 in cells upon treatment with proinflammatory agents TNF-α, IL-1α, and PMA. Western blot analysis was performed on whole cell extracts (30 μg lysate) of HeLa (lane 1), HeLa treated with IL-1α (20 ng/mL, 4 hr) (lane 2), HeLa treated with TNF-α (20 ng/mL, 12 hr) (lane 3), THP1 (lane 4), THP1 treated with PMA (80 nM, 12 hr) (lane 5), and PMA-differentiated THP1 macrophages treated with TNF-α (20 ng/mL, 12 hr) (lane 6). A 90 kDa band corresponding to TNFAIP3 was observed to be induced upon IL-1α or TNF-α treatment of HeLa (lanes 1–3) or TNF-α treatment of PMA-differentiated THP1 macrophages (lane 6). Detection was performed using the ECL Chemiluminescent Substrate Reagent Kit (Cat. No. WP20005) on the iBright FL1500 Imaging System (Cat. No. A44241).
Featured primary antibodies categories
Learn about our thousands of mouse, rat, hamster, rabbit, and other species of monoclonal primary antibodies.
Learn about our thousands of rabbit, goat, sheep, chicken, and other species of polyclonal primary antibodies.
Our broad portfolio of antibody conjugates—including Alexa Fluor dye and eFluor dye conjugates—is designed to help you answer complex cell biology questions, in less time and with less sample than other approaches.
Learn about our Invitrogen ABfinity rabbit monoclonal antibodies, developed by immunizing animals, screening for functionality, and then cloning the genes into high-level mammalian expression vectors.
Select a cell marker, organelle marker, or subcellular component antibody to identify and outline cellular structures and organelles by detecting proteins that are specific to them.
For Research Use Only. Not for use in diagnostic procedures.