A primary antibody is an immunoglobulin that specifically binds to a particular protein or other biomolecule of research interest for the purpose of purifying or detecting and measuring it. Primary antibodies are developed as polyclonal or monoclonal antibodies using mouse, rat, rabbit, goat, and other animal species as hosts. They are produced and supplied in various forms, ranging from crude antiserum to antigen-purified preparations. Primary antibodies for frequently researched targets are also available conjugated to fluorescent dyes or biotin.
The Invitrogen antibody portfolio is expanding – see the latest additions
We are continuously updating our Invitrogen primary antibody portfolio with advanced verified products for use in flow cytometry, IHC/IF/ICC, western blotting, ELISA, and other applications. Checkout our latest antibody highlights:
Testing the YY1 recombinant polyclonal antibody
The Invitrogen YY1 recombinant polyclonal antibody (Cat. No. 712089) was tested in three applications: immunofluorescence, western blot, and chromatin immunoprecipitation. siRNA-mediated knockdown was performed to demonstrate antibody specificity. Knockdown of YY1 was achieved by transfecting DU145 cells with YY1-specific siRNA. Loss of signal upon siRNA-mediated knockdown confirms that the antibody is specific to YY1.
Figure 1. YY1 recombinant polyclonal antibody (Cat. No. 712089) testing data. (A) Immunofluorescence analysis was performed in the prostate cancer cell model, DU145. Panel (a) shows staining of the antibody and panel (b) shows the composite image demonstrating nuclear localization of YY1. (B) Western blot analysis was performed on a panel of mammalian cell lines and YY1 was detected at 55kDa. (C) Knockdown of YY1 was achieved. (D) ChIP was performed in U-2 OS cells and enrichment was observed at promoters of CXXC1 and FAM193B (positive) but not at SAT2 and SAT alpha satellite repeats (negative).
Featured primary antibodies categories
Learn about our thousands of mouse, rat, hamster, rabbit, and other species of monoclonal primary antibodies.
Learn about our thousands of rabbit, goat, sheep, chicken, and other species of monoclonal primary antibodies.
Our broad portfolio of antibody conjugates—including Alexa Fluor dye and eFluor dye conjugates—is designed to help you answer complex cell biology questions, in less time and with less sample than other approaches.
Learn about our Invitrogen ABfinity rabbit monoclonal antibodies, developed by immunizing animals, screening for functionality, and then cloning the genes into high-level mammalian expression vectors.
For Research Use Only. Not for use in diagnostic procedures.