A primary antibody is an immunoglobulin that specifically binds to a particular protein or other biomolecule of research interest for the purpose of purifying or detecting and measuring it. Primary antibodies are developed as polyclonal or monoclonal antibodies using mouse, rat, rabbit, goat, and other animal species as hosts. They are produced and supplied in various forms, ranging from crude antiserum to antigen-purified preparations. Primary antibodies for frequently researched targets are also available conjugated to fluorescent dyes or biotin.
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We are continuously updating our Invitrogen primary antibody portfolio with advanced verified products for use in flow cytometry, IHC/IF/ICC, western blotting, ELISA, and other applications. Check out our latest antibody highlights:
ChIP validation of H3K4me3 recombinant antibody specific for histone modification
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromatin fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). Histone methylation is important for the formation of active and inactive regions of the genome and is crucial for the proper programming of gene expression during development.
H3K4me3 is an epigenetic modification of H3, characterized by trimethylation of the fourth lysine residue (Lys 4). It is one of the most widely studied histone modifications since it is associated with the promoters of active genes. Histone post-translational modifications (PTMs) such as methylation are often studied by chromatin immunoprecipitation (ChIP). It is, however, extremely challenging to develop ChIP antibodies specific to H3K4me3 (i.e., that do not recognize H3K4me1 or H3K4me2). Here we show that H3K4me3 Recombinant Polyclonal Antibody (9HCLC) (Cat. No. 711958) is specific to H3K4me3 and is validated* for the functionally relevant application (ChIP).
* The use or any variation of the word “validation” refers only to research use antibodies that were subject to functional testing to confirm that the antibody can be used with the research techniques indicated. It does not ensure that the product(s) was validated for clinical or diagnostic uses.
Tri-Methyl-Histone H3 (Lys 4) H3K4me3 Recombinant Polyclonal Antibody is specific to H3K4me3 and is validated for ChIP. (A) The specificity of the antibody to the H3K4me3 peptide was confirmed using the MODified Histone Peptide Array (Active Motif). Chemiluminescent detection was performed using Pierce ECL Western Blotting Substrate (Cat. No. 32106). The lower panel shows the dot blot data, and the top panel shows the analysis of the dot blot using Array Analyze Software (Active Motif). (B) Antibody specificity was also demonstrated by detection of enrichment of the target histone modification. A SNAP-ChIP assay was performed using the H3K4me3 recombinant polyclonal antibody, and H3K4me3 was enriched compared to the other histone modifications in the SNAP-ChIP K-MetStat Panel (EpiCypher). (C) ChIP was performed using the H3K4me3 Recombinant Polyclonal Antibody (9HCLC) (Cat. No. 711958, 5 μg) on sheared chromatin from 2 million HeLa cells, using the MAGnify Chromatin Immunoprecipitation System (Cat. No. 49-2024). Normal rabbit IgG was used as a negative control.
Featured primary antibodies categories
Learn about our thousands of mouse, rat, hamster, rabbit, and other species of monoclonal primary antibodies.
Learn about our thousands of rabbit, goat, sheep, chicken, and other species of polyclonal primary antibodies.
Our broad portfolio of antibody conjugates—including Alexa Fluor dye and eFluor dye conjugates—is designed to help you answer complex cell biology questions, in less time and with less sample than other approaches.
Learn about our Invitrogen ABfinity rabbit monoclonal antibodies, developed by immunizing animals, screening for functionality, and then cloning the genes into high-level mammalian expression vectors.
Select a cell marker, organelle marker, or subcellular component antibody to identify and outline cellular structures and organelles by detecting proteins that are specific to them.
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