A primary antibody is an immunoglobulin that specifically binds to a particular protein or other biomolecule of research interest for the purpose of purifying or detecting and measuring it. Primary antibodies are developed as polyclonal or monoclonal antibodies using mouse, rat, rabbit, goat, and other animal species as hosts. They are produced and supplied in various forms, ranging from crude antiserum to antigen-purified preparations. Primary antibodies for frequently researched targets are also available conjugated to fluorescent dyes or biotin.
The Invitrogen antibody portfolio is expanding—see the latest additions
We are continuously updating our Invitrogen primary antibody portfolio with advanced verified products for use in flow cytometry, IHC/IF/ICC, western blotting, ELISA, and other applications. Checkout our latest antibody highlights:
Rabbit recombinant monoclonal MAVS antibody for immuno-oncology research
MAVS (Mitochondrial antiviral-signaling protein), also known as virus induced signaling adaptor (VISA), IFN-β promoter stimulator I (IPS-1), or caspase activation recruitment domain adaptor inducing IFN-β (CARDIF), is a protein present in the outer membrane of mitochondria and peroxisomes. This protein plays a key role in innate immunity to viral infections. MAVS functions in RNA-sensing pathways, integrating signals from activated sensors, like RIG-1 and MDA5, upon viral infection and ultimately leading to proinflammatory cytokine production and programmed cell death of the infected cells. Recently, these pathways have been identified as targets for immune therapy in cancer, as it has been shown that activation of these pathways can directly induce programmed cell death in the cancer microenvironment.
Currently, few MAVS antibodies have been tested for their specificity. However, the recently launched Invitrogen MAVS Recombinant Rabbit Monoclonal Antibody (Cat. No. 703153) is CRISPR-verified to ensure specificity and has been tested in western blotting.
MAVS rabbit recombinant monoclonal antibody shows human reactivity and is knock-out verified. (A) Western blot analysis was performed using membrane-enriched extracts (30 µg lysate) of HeLa (lane 1), THP-1 (lane 2), A549 (lane 3), Hep G2 (lane 4), and NIH/3T3 (lane 5) cells. A ~60 kDa band corresponding to MAVS was observed only in the human cell lines tested. In Hep G2 cells, the MAVS antibody also recognizes a smaller isoform, evident by a smaller band at ~55 kDa. Detection was performed using the Invitrogen ECL Chemiluminescent Substrate Reagent Kit (Cat. No. WP20005). (B) Antibody specificity was demonstrated by CRISPR-Cas9-mediated knockout (KO) of the target protein in 293T cells. A loss of signal was observed for the target protein in a MAVS KO cell line compared to the Cas9 control cell line.
Featured primary antibodies categories
Learn about our thousands of mouse, rat, hamster, rabbit, and other species of monoclonal primary antibodies.
Learn about our thousands of rabbit, goat, sheep, chicken, and other species of monoclonal primary antibodies.
Our broad portfolio of antibody conjugates—including Alexa Fluor dye and eFluor dye conjugates—is designed to help you answer complex cell biology questions, in less time and with less sample than other approaches.
Learn about our Invitrogen ABfinity rabbit monoclonal antibodies, developed by immunizing animals, screening for functionality, and then cloning the genes into high-level mammalian expression vectors.
Select a cell marker, organelle marker, or subcellular component antibody to identify and outline cellular structures and organelles by detecting proteins that are specific to them.
For Research Use Only. Not for use in diagnostic procedures.