A primary antibody is an immunoglobulin that specifically binds to a particular protein or other biomolecule of research interest for the purpose of purifying or detecting and measuring it. Primary antibodies are developed as polyclonal or monoclonal antibodies using mouse, rat, rabbit, goat, and other animal species as hosts. They are produced and supplied in various forms, ranging from crude antiserum to antigen-purified preparations. Primary antibodies for frequently researched targets are also available conjugated to fluorescent dyes or biotin.
The Invitrogen antibody portfolio is expanding—see the latest additions
We are continuously updating our Invitrogen primary antibody portfolio with advanced verified products for use in flow cytometry, IHC/IF/ICC, western blotting, ELISA, and other applications. Check out our latest antibody highlights:
ChIP validation* of WDR5 recombinant antibody specific for histone modification
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromatin fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). Histone methylation is important for the formation of active and inactive regions of the genome and is crucial for the proper programming of gene expression during development.
WDR5 (WD repeat-containing protein 5) is part of a multiprotein complex (MLL1/MLL) that mediates methylation and acetylation of certain histone residues. Specifically, it positions the N-terminus of histone H3 for efficient trimethylation at Lys 4, which represents a specific tag for epigenetic transcriptional activation. WDR5 has been well-established as an oncogenic factor for MLL-rearranged leukemia. Recent studies have shown that WDR5 plays an important role in tumorigenesis and progression of a variety of cancers. WDR5 has been found to be considerably overexpressed in cancer cells, especially in cases of human pancreatic ductal adenocarcinomas and colon cancer cells.
* The use or any variation of the word “validation” refers only to research use antibodies that were subject to functional testing to confirm that the antibody can be used with the research techniques indicated. It does not ensure that the product(s) was validated for clinical or diagnostic uses.
WDR5 monoclonal antibody is specific and is validated for ChIP. (A) Immunofluorescence analysis was performed in HCT 116 cells using WDR5 Recombinant Rabbit Monoclonal Antibody (9H25L13) (Cat. No. 703745). The top panel shows staining of the antibody, and the bottom panel is a composite image where CNPase is shown to localize in the nucleus. (B) Knockdown of WDR5 was performed, where HCT 116 cells were transfected with WDR5 siRNA, and a decrease in signal intensity was observed via western blot. Chemiluminescent detection was performed using Pierce ECL Western Blotting Substrate (Cat. No. 32106). (C) ChIP was performed on sheared chromatin from 2 million HCT 116 cells using the MAGnify Chromatin Immunoprecipitation System (Cat. No. 49-2024). Normal rabbit IgG was used as a negative control. The purified DNA was analyzed by qPCR using primers binding to CDKN1A intron 1, the c-MYC promoter, the gene body (+2 kb) and transcriptional start site (TSS) of GAPDH, and SAT2 satellite repeats.
Featured primary antibodies categories
Learn about our thousands of mouse, rat, hamster, rabbit, and other species of monoclonal primary antibodies.
Learn about our thousands of rabbit, goat, sheep, chicken, and other species of polyclonal primary antibodies.
Our broad portfolio of antibody conjugates—including Alexa Fluor dye and eFluor dye conjugates—is designed to help you answer complex cell biology questions, in less time and with less sample than other approaches.
Learn about our Invitrogen ABfinity rabbit monoclonal antibodies, developed by immunizing animals, screening for functionality, and then cloning the genes into high-level mammalian expression vectors.
Select a cell marker, organelle marker, or subcellular component antibody to identify and outline cellular structures and organelles by detecting proteins that are specific to them.
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For Research Use Only. Not for use in diagnostic procedures.