Aromatase, a product of the CYP19A1 gene, is a member of the cytochrome P450 superfamily of monooxygenases that catalyze numerous reactions involved in drug metabolism and the synthesis of steroids, cholesterol, and other lipids. Aromatase is localized on the endoplasmic reticulum and catalyzes the formation of aromatic C18 estrogens from C19 androgens.

Aromatase is found in many tissues including the adrenal glands, gonads, placenta, skin, and adipose, as well as several abnormal tissues including those associated with endometriosis, uterine fibroids, breast cancer, and endometrial cancer. A surplus of aromatase leads to aromatase excess syndrome (AES), in which excess levels of estrogen result in heterosexual precocity in males and isosexual precocity in females. Furthermore, a lack of aromatase leads to aromatase deficiency (AROD), a rare disease in which fetal androgens are not converted into estrogens because of placental aromatase deficiency. This deficiency causes the virilization of female fetuses and primary amenorrhea, polycystic ovaries, breast hypoplasia, and tall stature in adult females.

Invitrogen aromatase antibodies are validated for use in various applications. To see all the aromatase antibody offerings please click the link below.

See all aromatase antibodies

Applications

Aromatase antibodies are commonly used in research applications such as western blot, immunohistochemistry, and immunofluorescence. Quality Invitrogen aromatase receptor antibodies are available for a variety of research needs.

Western blot analysis of aromatase

Western Blot analysis of Aromatase using a Aromatase Monoclonal antibody. Aromatase Monoclonal antibody (Cat. No. MA5-33132) used at a concentration of 2.3 µg/mL. Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, SH-SY5Y whole cell lysate, U87 whole cell lysate, K562 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 58 kDa.

Immunohistochemical analysis of aromatase PA1-21398

Immunohistochemical analysis of aromatase using a specific aromatase polyclonal antibody (Cat. No. PA1-21398).