Golgi Marker Antibodies, PA3-900

The golgi complex is a key organelle in the secretory pathway of the cell. It receives proteins from the ER (endoplasmic reticulum) and processes and sorts them for further transport to lysosomes, the plasma membrane, or back to the ER.

The golgi complex is composed of flattened stacks of membranes (cisternae) organized into four functionally distinct areas: the cis-Golgi network, the medial cisternae, the trans cisternae, and the trans-Golgi network. Each region of the golgi complex performs distinct processing and sorting events as proteins enter at the cis face and exit from the concave trans face. The golgi complex also prepares other macromolecules such as lipids and carbohydrates for secretion from the cell or for use by other organelles within the cell.

Each golgi cisternae has specific markers. Golgi marker antibodies detect proteins specific to the golgi complex and can aid in the study of the dynamics and morphology of each part of the golgi complex. Golgi marker antibodies can also help elucidate the role or roles a protein may play in a number of tasks that are centered in or influenced by the golgi. Quality Invitrogen golgi marker antibodies are available for a variety of research needs.

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Immunofluorescence analysis of Golgi in A549 cells

Immunofluorescence analysis of Golgi in A549 cells. A549 cells were left untreated or treated with indicated amount of Brefeldin A for 30 min. Cells were fixed, permeabilized, and blocked using the Image-iT Fixation/Permeabilization Kit (Cat. No. R37602). Golgi were labeled with an anti-golgin 97 antibody (Cat. No. A-21270) at 1:300 dilution, followed by detection with an Alexa Fluor 647 Goat anti mouse secondary (Cat. No. A-21235) at 1:2000 dilution. Cells were co stained with GlcNaz (detected with Alexa FluorTM 488 Alkyne). Nuclei (blue) were stained with Hoechst 33342 dye (Cat. No. 62249). Images were acquired with a 20x objective on the Thermo Arrayscan VTi. Analysis was performed using compartmental analysis to identify Golgin 97 positive structures (spots) in a perinuclear ring.