Ribosomes are non-membranous organelles made of complexes of rRNA and proteins that function as the primary location of protein synthesis. Ribosomes consist of two subunits: the smaller 40S subunit binds and reads the mRNA, and the larger 60S subunit binds the tRNA and amino acids. The two subunits separate when a ribosome finishes reading an mRNA molecule.
Ribosomal marker antibodies can aid in the study of ribosome dynamics and morphology. Ribosomal marker antibodies can also help elucidate the role or roles a protein may play in a number of tasks that are centered in or influenced by the ribosome. Faulty ribosome biogenesis and function can result in a number of diseases, and ribosomal marker antibodies may be used in studying these maladies. Invitrogen ribosomal marker antibodies are designed to dependably detect the key ribosomal targets. Each antibody is validated for use in various applications. Key ribosomal maker targets include:
Immunohistochemistry performed on normal biopsies of deparaffinized mouse liver tissue. To expose target proteins, heat-induced antigen retrieval was performed using 10 mM sodium citrate (pH 6.0) buffer, microwaved for 8–15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a KDEL rabbit polyclonal antibody (Cat. No. PA1-013) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunofluorescent analysis of Ribosomal Protein S3 in HeLa cells using a Ribosomal Protein S3 polyclonal antibody (Cat. No. PA5-17214) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.
For Research Use Only. Not for use in diagnostic procedures.