Ribosomal Marker Antibodies, PA1-013

Ribosomes are non-membranous organelles made of complexes of rRNA and proteins that function as the primary location of protein synthesis. Ribosomes consist of two subunits: the smaller 40S subunit binds and reads the mRNA, and the larger 60S subunit binds the tRNA and amino acids. The two subunits separate when a ribosome finishes reading an mRNA molecule.

Ribosomal marker antibodies detect proteins specific to the ribosomes and can aid in the study of the morphology and dynamics of the ribosome. Ribosomal marker antibodies can also help elucidate the role or roles a protein may play in a number of tasks that are centered in or influenced by the ribosome. Faulty ribosome biogenesis and function can result in a number of diseases, and ribosomal marker antibodies may be used in studying these maladies. Quality Invitrogen ribosomal marker antibodies are available for a variety of research needs.

Ribosomal marker antibody targets

  • AGO2
  • CANX
  • EIF2S1
  • FBL
  • SNCA
  • GRP78/BIP
  • HSPA5
  • MTOR
  • NCL
  • NPM1
  • OXA1L
  • p70 S6 Kinase
  • PTEN
  • Ribosomal Protein
  • RPL26
  • RPL7A
  • RPS27A
  • RPS3
  • RPS6
  • RPS6KB1
  • SEC61A1
  • Ubiquitin

Featured product data

Immunofluorescent analysis of SNCA on 70% confluent log phase HeLa cells.

Immunofluorescent analysis of SNCA was performed on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0. 25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ABfinity SNCA recombinant rabbit monoclonal antibody (Cat. No. 701085) at a dilution of 1:1000 in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 goat anti-rabbit IgG secondary antibody (Cat. No. A11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade Gold Antifade Mountant with DAPI (Cat. No. S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 phalloidin (Cat. No. A12381) and Panel d is a merged image showing nuclear localization and panel e is a control without primary antibody. The images were captured using a Nikon microscope at 20X magnification.

Immunohistochemistry performed on normal biopsies of deparaffinized mouse liver tissue.

Immunohistochemistry performed on normal biopsies of deparaffinized mouse liver tissue. To expose target proteins, heat-induced antigen retrieval was performed using 10 mM sodium citrate (pH 6.0) buffer, microwaved for 8–15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a KDEL rabbit polyclonal antibody (Cat. No. PA1-013) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Annotated product references

Cat. No. PA1-013 was used in immunohistochemistry to investigate the role of proteiun degradation in the pathogenesis of chronic obstructive pulmonary disease and severe emphysema. Journal of molecular medicine (Berlin, Germany) (Jun 2011; 89: 577) "Critical role of proteostasis-imbalance in pathogenesis of COPD and severe emphysema." Min T,Bodas M,Mazur S,Vij N

Cat. No. PA1-014A was used in western blot to study the effect of Ras on endoplasmic reticulum stress in human cancer cells and its mechanism. International journal of cancer. Journal international du cancer (May 2010; 126: 2268) "Ras inhibits endoplasmic reticulum stress in human cancer cells with amplified Myc." Yaari-Stark S,Shaked M,Nevo-Caspi Y,Jacob-Hircsh J,Shamir R,Rechavi G,Kloog Y