Most protein and cell analysis techniques involving antibodies depend upon using target-specific primary antibodies and labeled, conjugated secondary antibodies. However, using directly labeled primary antibodies enhances or simplifies certain procedures. For example, commercially available ELISA kits typically use matched pairs of target-specific (primary) antibodies in which the detector antibody of the pair is biotinylated. This arrangement enables sensitive and reliable signal generation, and amplification occurs by probing the biotin label with HRP-conjugated streptavidin.

Related product and application categories


Find a target-specific ELISA kit. Also learn about our matched antibody pairs and numerous singleplex and multiplex assays for Luminex® Instruments, most of which use biotinylated detector antibodies.

Antibody Labeling

Can’t find a conjugated version of the primary antibody that interests you? Discover the variety of convenient and powerful options for labeling and conjugating antibodies (or other proteins) on your own.

Cell Analysis

Browse the many cell imaging and analysis techniques that rely on the use of primary antibodies, including those that are directly conjugated to fluors or biotin.

Secondary Antibodies

Learn about our wide selection of conjugated and unconjugated secondary antibodies for detection of mouse, rabbit and many other species and isotypes of primary antibodies.