Fluorescent proteins are highly versatile biological markers for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. We offer anti-GFP, anti-RFP, and anti-mCherry antibodies for use in detecting these powerful tools, unlabeled and as antibody conjugates for use a wide variety of applications, including imaging, western blotting, and flow cytometry.

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Sensitive and specific detection

Anti-GFP, anti-RFP, and anti-mCherry antibodies provide a convenient method for visualizing GFP, RFP, or mCherry proteins respectively, especially when amplification of the fluorescent protein of interest is necessary to overcome a weak or degraded signal.

Our anti-fluorescent protein antibodies are highly specific, reacting only with the intended target and thereby contributing to low background signal and an increased signal to noise ratio. They are easily incorporated into standard immunostaining protocols for cell and tissue analysis,enabling consistent and reliable detection of fluorescent proteins and fluorescent protein fusions in western blot and flow cytometry applications. 

Validated for a variety of applications

Our antibodies have been validated for a broad range of applications and targets. The following figure demonstrates the use of our anti-GFP monoclonal antibody in helping to improve visualization by enhancing the dim GFP fluorescence in an immunostaining experiment.

Immunolabeling enhances GFP fluorescence. HeLa cells were transduced with CellLight™ Lysosomes-GFP, then fixed and permeabilized. GFP, localized in the lysosomes, was labeled with anti-GFP rabbit monoclonal antibody and detected with Alexa Fluor™ 594 goat anti–rabbit IgG (H+L) (red). The nucleus was stained with DAPI (blue), and the sample was mounted using ProLong™ Gold antifade reagent. (Left panel) Some GFP fluorescence was retained in the lysosomes after fixation. (Center panel) Immunolabeling and detection greatly improved visualization by enhancing the dim GFP fluorescence. (Right panel) The merged yellow signal indicates colocalization of GFP fluorescence and the detection antibody.