Detection via antibodies specific for green fluorescent protein (GFP)

The green fluorescent protein (GFP) from the jellyfish Aequorea victoria is a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression. We offer anti-GFP antibody conjugates as a rabbit IgG fraction and chicken IgY fraction conjugated to biotin, horseradish peroxidase (HRP), or fluorophore labeled. All anti-GFP antibody conjugates are suited for detection of native GFP, GFP variants, and most GFP fusion proteins by western blot analysis and immunocytochemistry. The anti-GFP rabbit polyclonal antibody is raised against GFP isolated directly from Aequorea victoria and the IgG fraction is purified by ion-exchange chromatography.

Validated for a variety of applications

  • Western analysis
  • Immunocytochemistry
  • Immunoprecipitation
  • Flow cytometry
  • Immunohistochemistry
Detection using Alexa Fluor 488-conjugated anti-GFP antibody

Anti-GFP conjugated to Alexa Fluor 488 with target BacMam-GFP

Alexa Fluor 488 dye was conjugated to two independent lots of GFP Rabbit IgG Polyclonal antibody resulting in Alexa Fluor 488-conjugated anti-GFP antibody (Cat. No. A-21311). U2OS cells were transduced with CellLight Mitochondria-GFP, BacMam 2.0 (Cat. No. C10508). After an overnight incubation the cells with treated with fixation and then permeablized. GFP molecules were detected by the addition of anti-GFP Alexa Fluor 488 (Cat. No. A-21311). Texas Red-X phalloidin (Cat. No.T7471) was added to detect actin. Hoechst 33342 (Cat. No. H3570) was used as a nuclear counterstain.

Recovering GFP signal after Click-iT EdU treatment

Human osteosarcoma cells transduced with CellLight Mitochondria-GFP (Cat. No. C10508) samples were pulsed with EdU, fixed, and labeled with Click-iT EdU Alexa Fluor 555  (Cat. No. C10338) for cell proliferation (pink). DAPI for nuclei (blue) (Cat. No. D3571). GFP signal was recovered with anti-GFP Alexa Fluor 488 (Cat. No. A-21311) primary antibody (green).

Detection via fluor-conjugated anti-GFP antibody