Detection using antibodies specific for red fluorescent protein (RFP)

RFP (Red Fluorescent Protein) comes in many forms including tdTomato, mCherry, mStrawberry, and DsRed. RFP produces a bright red fluorescence when exposed to blue light, and the RFP tag is widely used to monitor protein localization and gene expression in living cells.

RFP tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe. Invitrogen RFP tag antibodies reliably detect multiple forms of RFP on tagged proteins, and each antibody is validated for use in a variety of applications.

See all anti-RFP tag antibodies

Featured product data

Immunofluorescent analysis of recombinant red fluorescent protein (RFP)-transfected HeLa cells. Cells were transfected with a pCMV RFP C-Myc plasmid, and 48 hours post-transfection cells were fixed with formalin and permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were blocked with 3% BSA for 15 minutes at room temperature, and then probed with an anti-RFP polyclonal antibody (Cat. No. PA1-986) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat anti-rabbit IgG secondary antibody (Cat. No. 35553) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Cat. No. 62249). Images were taken on a Thermo Scientific ArrayScan imager 20x magnification.

Flow cytometry analysis of red fluorescent protein (RFP) using a specific antibody. Fixed and permeabilized HEK293 cells were transduced by CellLight Tubulin-RFP (Cat. No. C10614, right peak, orange), and quenched by an RFP-specific recombinant rabbit oligoclonal antibody (Cat. No. 710530, middle peak, filled). Non-transduced cells are shown in blue (left peak).

Annotated product references

Cat. No. PA1-986 was used in western blot to study the role of miR-122 and cyclin G1 in the mechanism by which ethanol promotes the replication of HCV RNA. Alcoholism, clinical and experimental research ( 2013; 37: 599) "Alcohol facilitates HCV RNA replication via up-regulation of miR-122 expression and inhibition of cyclin G1 in human hepatoma cells." Hou W,Bukong TN,Kodys K,Szabo G