Green fluorescent protein (GFP) is a 27 kDa monomeric protein, isolated from the jellyfish Aequorea Victoria that auto-catalytically forms a fluorescent pigment. GFP is a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression. GFP is also a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP brightly fluoresces green (peak emission 508nm) in a stable, species independent manner when exposed to UV or blue light (395nm) making it ideal as a marker for use in a variety of applications such as fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Our anti-GFP antibodies are highly specific; react only with the intended target and in so doing contribute to low background signal and increased signal to noise ratio. All our anti-GFP antibody conjugates are suited for detection of native GFP, GFP variants, and most GFP fusion proteins by western blot analysis and immunocytochemistry.

Applications

Our anti-GFP antibodies are validated for a variety of applications such as western analysis, immunocytochemistry, immunoprecipitation, flow cytometry and immunohistochemistry.

Immunoflourescence detection of GFP using anti-GRP Alexa Fluor 488 antibody

Alexa Fluor 488 dye was conjugated to two independent lots of GFP Rabbit IgG Polyclonal antibody resulting in Alexa Fluor 488-conjugated anti-GFP antibody (Cat. No. A-21311). U2OS cells were transduced with CellLight Mitochondria-GFP, BacMam 2.0 (Cat. No. C10508). After an overnight incubation the cells with treated with fixation and then permeablized. GFP molecules were detected by the addition of anti-GFP Alexa Fluor 488 (Cat. No. A-21311). Texas Red-X phalloidin (Cat. No. T7471) was added to detect actin. Hoechst 33342 (Cat. No. H3570) was used as a nuclear counterstain.

Human osteosarcoma cells transduced with CellLight Mitochondria-GFP (Cat. No. C10508) samples were pulsed with EdU, fixed, and labeled with Click-iT EdU Alexa Fluor 555 (Cat. No. C10338) for cell proliferation (pink). DAPI for nuclei (blue) (Cat. No. D3571). GFP signal was recovered with anti-GFP Alexa Fluor 488 (Cat. No. A-21311) primary antibody (green).

Immunofluorescence detection of GFP using anti-GFP Alexa Fluor 488 primary antibody