Green fluorescent protein (GFP) is a 27 kDa monomeric protein, isolated from the jellyfish Aequorea Victoria that auto-catalytically forms a fluorescent pigment. GFP is a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression. GFP is also a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP brightly fluoresces green (peak emission 508nm) in a stable, species independent manner when exposed to UV or blue light (395nm) making it ideal as a marker for use in a variety of applications such as fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Our anti-GFP antibodies are highly specific; react only with the intended target and in so doing contribute to low background signal and increased signal to noise ratio. All our anti-GFP antibody conjugates are suited for detection of native GFP, GFP variants, and most GFP fusion proteins by western blot analysis and immunocytochemistry.
Alexa Fluor 488 dye was conjugated to two independent lots of GFP Rabbit IgG Polyclonal antibody resulting in GFP Polyclonal Antibody, Alexa Fluor 488 (Cat. No. A-21311). U2OS cells were transduced with CellLight Mitochondria-GFP, BacMam 2.0 (Cat. No. C10508). After an overnight incubation the cells with treated with fixation and then permeabilized. GFP molecules were detected by the addition of GFP Polyclonal Antibody, Alexa Fluor 488 (Cat. No. A-21311). Texas Red-X phalloidin (Cat. No. T7471) was added to detect actin. Hoechst 33342, Trihydrochloride, Trihydrate (Cat. No. H3570) was used as a nuclear counterstain.
Human osteosarcoma cells transduced with CellLight Mitochondria-GFP, MacMam 2.0 (Cat. No. C10508) samples were pulsed with EdU, fixed, and labeled with Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 555 (Cat. No. C10338) for cell proliferation (pink).DAPI for nuclei (blue) (Cat. No. D3571). GFP signal was recovered with GFP Polyclonal Antibody, Alexa Fluor 488 (Cat. No. A-21311) primary antibody (green).