Red fluorescent protein (RFP) is a biological marker used for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. RFP comes in many forms including tdTomato, mCherry, mStrawberry, and DsRed and produces a bright red fluorescence when exposed to blue light.Anti-RFP antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe. They provide a convenient method for visualizing RFP, especially, when amplification of the fluorescent protein of interest is necessary to overcome a weak or degraded signal. Invitrogen RFP tag antibodies reliably detect multiple forms of RFP on tagged proteins and each antibody is tested for use in a variety of applications.

Applications

Invitrogen anti-RFP antibodies are designed to dependably detect RFP in various applications.

Immunofluorescence using anti-RFP antibody to stain cells

Immunofluorescence using an anti-RFP antibody. HeLa cells were incubated with Organelle Lights Mito-RFP (O36229) and Organelle Lights Golgi-GFP (O10098). Following fixation and permeabilization, cells were stained with Alexa Fluor 350 phalloidin (Cat. No. A22281) and anti-RFP rabbit polyclonal antibody (Cat. No. R10367), the latter subsequently visualized with Alexa Fluor 647 Goat Anti-Rabbit IgG antibody (Cat. No. A-21245, red fluorescence) and imaged. No cross-reactivity is observed for the anti-RFP antibody with GFP (green-fluorescence, perinuclear region).