Red fluorescent protein (RFP) is a biological marker used for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. RFP comes in many forms including tdTomato, mCherry, mStrawberry, and DsRed and produces a bright red fluorescence when exposed to blue light.Anti-RFP antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe. They provide a convenient method for visualizing RFP, especially, when amplification of the fluorescent protein of interest is necessary to overcome a weak or degraded signal. Invitrogen RFP tag antibodies reliably detect multiple forms of RFP on tagged proteins and each antibody is tested for use in a variety of applications.
Invitrogen anti-RFP antibodies are designed to dependably detect RFP in various applications.
Immunofluorescence using an anti-RFP antibody. HeLa cells were incubated with Organelle Lights Mito-RFP (O36229) and Organelle Lights Golgi-GFP (O10098). Following fixation and permeabilization, cells were stained with Alexa Fluor 350 phalloidin (Cat. No. A22281) and anti-RFP rabbit polyclonal antibody (Cat. No. R10367), the latter subsequently visualized with Alexa Fluor 647 Goat Anti-Rabbit IgG antibody (Cat. No. A-21245, red fluorescence) and imaged. No cross-reactivity is observed for the anti-RFP antibody with GFP (green-fluorescence, perinuclear region).
Flow cytometry analysis of red fluorescent protein (RFP) using a specific antibody. Fixed and permeabilized HEK293 cells were transduced by CellLight Tubulin-RFP (Cat. No. C10614, right peak, orange), and quenched by an RFP-specific recombinant rabbit oligoclonal antibody (Cat. No. 710530, middle peak, filled). Non-transduced cells are shown in blue (left peak).
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