Monoclonal antibodies used in immunofluorescence

Monoclonal antibodies are produced by injecting an antigen into a host animal to initiate a humoral immune response. In most procedures, spleen cells from these hosts are fused in vitro with cultured malignant myeloma cells. Unique cell clones are isolated and those that survive the fusion step are known as hybridomas. Hybridomas are immortal because of their myeloma characteristics and are easily propagated in culture. Because of their B cell properties, some hybridoma clones continue to synthesize and secrete genetically homogeneous antibodies against a single epitope, called monoclonal antibodies. Monoclonal antibodies are homologous to natural immunoglobulins from the source immunized animal, but unlike polyclonal antibodies purified from serum, they are specific to a single epitope and provide a stable long term supply of produced by hybridomas in vitro. We offer a wide range of monoclonal antibodies for use in various applications. 

Featured antibody related categories

Recombinant rabbit monoclonal antibodies are developed by immunizing animals and then cloning the immunogen-specific antibody genes into high-level mammalian expression vectors.

View our wide selection of antibody and protein labeling kits for the attachment of fluorescent dyes and labels, including Alexa Fluor dyes, Qdot labels, and biotin.

Explore our diverse products for Immunoassays including matched antibody pairs, uncoated and coated-plate ELISA kits and multiplexing assay for the Luminex Platform.

Rat and mouse hybridoma development yields custom monoclonal antibodies from synthesized peptide or recombinant protein antigens.

Rat and mouse hybridoma development yields custom monoclonal antibodies from synthesized peptide or recombinant protein antigens.


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