The cytoplasm consists of cytosol and organelles. Cytosol contains water, salts, and organic molecules such as cytoskeleton filaments and ribosomes. All organelles in the cell are suspended in the cytoplasm with cytosol surrounding them. The cytoplasm is 70% of a cell’s volume and is where most cellular activities occur. Cytoplasm marker antibodies detect proteins specific to the cytoplasm and can aid in the study of the morphology and function of the cytoplasm. Cytoplasm marker antibodies can also help elucidate the roles a protein may play in a number of tasks that are centered in or influenced by the cytoplasm.
Invitrogen cytoplasm marker antibodies are designed to dependably detect the key cytoplasm targets. Each antibody is validated for use in various applications. Key cytoplasm targets include:
Each Invitrogen cytoplasm marker antibody is validated for use in applications such as ELISA, western blot, flow cytometry, immunofluorescence, immunohistochemistry, immunocytochemistry, and immunoprecipitation.
Immunofluorescent analysis of Vimentin using Vimentin Monoclonal antibody (J144) (Cat. No. MA3-745) shows staining in C6 glioma cells. Vimentin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Vimentin (Cat. No. MA3-745) at a dilution of 1:100-1:200 overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Cat. No. 35552 for GAR, Cat. No. 35503 for GAM). Images were taken at 60X magnification.
Immunofluorescent analysis of HIF-1 alpha using an antibody recognizing HIF-1 alpha shows staining in U251 cells. HIF-1 alpha (green), F-actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (negative control) or with HIF-1 alpha monoclonal antibody (Cat. No. MA1-516) at a dilution of 1:20 overnight at 4°C, washed with PBS and incubated with a DyLight 488−conjugated secondary antibody (Cat. No. 35552 for GAR; Cat. No. 35503 for GAM). Images were taken at 60x magnification.
Immunohistochemistry performed on normal deparaffinized human heart tissue. To expose target proteins, heat-induced antigen retrieval was performed using 10 mM sodium citrate (pH 6.0) buffer, microwaved for 8–15 minutes. Following antigen retrieval, tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing iNOS (Cat. No. PA1-036) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
For Research Use Only. Not for use in diagnostic procedures.