Recombinant Antibodies

Our recombinant antibodies include antibodies generated using the ABfinity technology platform and phage display.

No matter how they are produced, recombinant antibodies are derived from DNA. This is beneficial because there are fewer animal immunizations needed, leading to less lot-to-lot variability. Instead, each new lot of recombinant antibody comes straight from the same genetic source and has the same bioactivity, specificity, and quality as the previous lots.

Other recombinant antibody advantages include:

  • High specificity
  • High sensitivity
  • Animal origin–free

Data generated using recombinant antibodies


Immunofluorescence on methanol-fixed MDA-MB-231 cells for detection of WNT2B/13. Experiment was performed using anti-WNT2B/13 recombinant rabbit monoclonal antibody (Cat No. 701856, 2 µg/mL), alpha-tubulin monoclonal antibody (Cat. No. 322500, 1 µg/mL) and labeled with goat anti–rabbit IgG (H+L) Superclonal™ secondary antibody, Alexa Fluor™ 488 conjugate (Cat. No. A27034, 1:2000) and goat anti–mouse IgG secondary antibody, Alexa Fluor™ 594 conjugate (Cat. No. A11032, 1:400), respectively. (a); Representative cells stained for detection and localization of WNT2B/13 protein (green). (b) Nuclear staining (blue) using Invitrogen™ SlowFade™ Gold Antifade Mountant with DAPI (Cat. No. S36938). (c) Cytoskeletal alpha-tubulin staining (red). (d) Composite image of a, b, and c, clearly demonstrating cytoplasmic localization of WNT2B/13. (e) Control cells with no primary antibody, for assessment of background.

Western blot analysis of AKT [pS473]

Western blot analysis of AKT [pS473]. Experiment was performed with 20 µg of cell lysates from NIH/3T3 (lane 1), NIH/3T3 treated for 10 min with 25 ng/mL of PDGF (lane 2), and U-87 MG (lane 3), and using an Invitrogen™ NuPAGE™ 4-12 % Bis-Tris gel (Cat. No. NP0321BOX), Invitrogen™ XCell SureLock™ Electrophoresis System (Cat. No. EI0002), Invitrogen™ Sharp Pre-Stained Protein Standard (Cat. No. LC5800), and Invitrogen™ iBlot™ Dry Blotting System (Cat. No. IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk for 1 hr at room temperature. AKT [pS473] was detected (~55 kDa) using ABfinity™ AKT [pS473] recombinant rabbit monoclonal antibody (Cat. No. 700392) at 1–3 µg/mL in 2.5% skim milk at 4°C overnight on a rocking platform. To confirm specificity, competition was performed with phosphopeptide (10 µg/mL) as shown in the blot on the right. Goat anti-rabbit IgG-HRP secondary antibody (Cat. No. G21234) at 1:5,000 dilution was used, and chemiluminescent detection was performed using the Invitrogen™ ECL Chemiluminescent Substrate Reagent Kit (Cat. No. WP20005).

Flow cytometry analysis of BRD3 on U-87 MG cells

Flow cytometry analysis of BRD3 on U-87 MG cells. Cells were fixed with 70% ethanol for 10 min, permeabilized with 0.25% Triton™ X-100 for 20 min, and blocked with 5% BSA for 30 min at room temperature. Cells were labeled with BRD3 mouse recombinant monoclonal antibody (Cat. No. 730024, red histogram) or with mouse isotype control (yellow histogram) at 3–5 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 hr, the cells were labeled with Alexa Fluor™ 488 rabbit anti-mouse secondary antibody (Cat. No. A11059) at a dilution of 1:400 for 30 min at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using the Invitrogen™ Attune™ Acoustic Focusing Cytometer. The purple histogram represents unstained control cells, and the green histogram represents the control with no primary antibody.

Immunohistochemistry analysis of IRAK4

Immunohistochemistry analysis of IRAK4. Staining in the cytoplasm of paraffin-embedded human cervix tissue (right) is compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8–15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with an IRAK4 ABfinity™ rabbit monoclonal antibody (Cat. No. 700026) diluted in 3% BSA-PBS at a dilution of 1:20 overnight, at 4°C in a humidified chamber. Tissues were washed extensively in PBST, and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.