There are a wide variety of protein targets important when studying neurobiology. These targets are important in the study of glia, protein trafficking, neurogenesis, axon guidance, dendrite development, neuronal migration, growth factors, neuromuscular junction, neurotrophins, apoptosis, and synaptogenesis. We have a diverse array of highly specific antibodies for neuroscience research that have been validated* for multiple applications. These applications include western blot, flow cytometry, immunofluorescence, ELISA, and immunohistochemistry.
Neurobiology antibodies cover a wide range of targets and markers depending on the type of research being done. Listed below are some of the most prevalent markers that are studied.
Our growing portfolio of traditional and recombinant antibodies is designed to enable detection and characterization of neurobiology targets with exceptional specificity.
Immunofluorescent analysis of A549 cells using a FOXG1 polyclonal antibody (Cat. No. PA5-26794). A549 cells were fixed with 4% PFA (20 minutes), permeabilized with Triton X-100 (0.1%, 10 minutes), then incubated with a FOXG1 polyclonal antibody (Cat. No. PA5-26794) (1:25, 1 hour at 37°C). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 minutes at 37°C). Actin filaments have been labeled with dye-conjugated phalloidin (red). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 minutes).
Chromatin immunoprecipitation analysis of Pax3. Performed using cross-linked chromatin from 1 x 10^6 HCT116 human colon carcinoma cells treated with serum for 0, 15, and 60 minutes. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay with 1.0 µL/100 µL well volume of a Pax3 Recombinant Rabbit Polyclonal Antibody (Cat. No. 710178). Chromatin aliquots from ~1 x 10^5 cells were used per ChIP pull-down. Quantitative PCR data were done in quadruplicate using 1 µL of eluted DNA in 2 µL SYBR real-time PCR reactions containing primers to amplify-3.2kb upstream of human FOS, exon-4 of FOS, or the imprinting control region (ICR) of the human H19 locus. PCR calibration curves were generated for each primer pair from a dilution series of sheared total genomic DNA. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM for three experiments. A schematic representation of human FOS and H19 loci are shown above the data where boxes represent exons (grey boxes = translated regions, white boxes = untranslated regions), the zigzag lines represent introns, and the straight lines represent upstream sequences. Regions amplified by FOS and H19 primers are represented by black bars.
Western blot analysis on membrane enriched extract (30 µg) of HEK 293 (Lane 1), SK-N-AS (Lane 2), SH-SY5Y (Lane 3) and U-87 MG (Lane 4). The blots were probed with Anti-beta-Amyloid Rabbit Polyclonal Antibody (Cat. No. 36-6900, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Cat. No. A27036, 0.4 µg/mL 1:2500 dilution). A ~ 35 kDa band corresponding to beta-Amyloid was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex NuPAGE 12% Bis-Tris gel (Cat. No. NP0342BOX), XCell SureLock Electrophoresis System (Cat. No. EI0002) and Novex Sharp Pre-Stained Protein Standard (Cat. No. LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with Pierce Power Blotter System (Cat. No. PB0012). The membrane was probed with the relevant primary and secondary Antibody using iBind Flex Western Starter Kit (Cat. No. SLF2000S). Chemiluminescent detection was performed using Pierce ECL Western Blotting Substrate (Cat. No. 32106).
*The use or any variation of the word “validation” refers only to research use antibodies that were subject to functional testing to confirm that the antibody can be used with the research techniques indicated. It does not ensure that the product(s) was validated for clinical or diagnostic uses
For Research Use Only. Not for use in diagnostic procedures.