Fluorescent-dye conjugated secondary antibodies provide a much needed tool for identifying proteins in many applications including fluorescent cell imaging, western blotting, immunohistochemistry and more. The advantages of using a fluorescently labeled secondary antibody include brighter signal, multiplexing capabilities, and ease of use (many are available for sale pre-conjugated to many different color of dyes). Thermo Fisher Scientific offers a wide selection of fluorescent-dye conjugated secondary antibodies for your research, with over 60 different fluorescent dyes and dye combinations, and nanocrystals in a wide range specific target and host species.

How to find a secondary antibody

Step 1: Search for the target species of interest (e.g., “anti-mouse” or “goat anti-rabbit” or “alexa fluor 488”).
Step 2: Narrow results by host species, conjugate, application, and other criteria using side filters.

Popular fluorescent secondary antibodies

Goat anti-Mouse IgG (H+L) Secondary Antibody
Donkey anti-Rabbit IgG (H+L) Secondary Antibody

Fluorescent secondary antibody selection guide

Featured fluorescent secondary antibody categories

Select from 18 different individual Alexa Fluor™ dyes and 4 Alexa Fluor tandem dyes as secondary antibody conjugates. Alexa Fluor secondary antibodies exhibit superior brightness and photostability, outperforming conventional fluorescent secondary antibodies. 

Find secondary antibody conjugates of traditional fluorophores such as fluorescein (FITC), tetramethylrhodamine (TRITC), Rhodamine Red™, Cy™3, Cy™5, and Texas Red™ dyes.

Alexa Fluor Secondary Antibodies

Alexa Fluor™ conjugates exhibit more intense fluorescence than other spectrally similar conjugates. The superior performance of Alexa Fluor dyes means you need less conjugate for optimal results. See a comparison of working concentration of selected secondary conjugates.

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