Invitrogen Alexa Fluor Plus secondary antibodies

Make your low-abundance targets visible, spend less time optimizing, and make every one of your precious samples count. Invitrogen Alexa Fluor Plus conjugate technology provides higher signal to noise ratios with minimized cross-reactivity for fluorescent imaging and western blotting.

See Alexa Fluor Plus antibodies

Secondary antibody conjugates of Alexa Fluor 488 vs. Alexa Fluor Plus 488
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Alexa Fluor Plus 488 allows you to see greater detail compared to traditional Alexa Fluor 488 conjugates. Traditional Alexa Fluor 488 secondary antibody (left) compared to Alexa Fluor Plus 488 secondary antibody (right, goat anti-mouse IgG secondary antibody, Cat. No. A32723) in immunofluorescent analysis of β-III tubulin in E18 Sprague Dawley primary cortical neuronal cells. Nuclei were stained with Hoechst 33342 stain (Cat. No. H3570). The image contains overlay of neuronal tubulin (green) and nuclei (blue). Images were taken on a confocal microscope at 40x magnification.

Product list: Alexa Fluor Plus 488 Secondary Antibodies
Goat anti-rabbit A32731
Goat anti-mouse A32723
Goat anti-chicken A32931
Donkey anti-goat A32814
Donkey anti-rabbit A32790
Donkey anti-mouse A32766
Secondary antibody conjugates of Alexa Fluor 555 vs. Alexa Fluor Plus 555
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Alexa Fluor Plus 555 allows you to see greater detail compared to traditional Alexa Fluor 555 conjugates. Traditional Alexa Fluor 555 secondary antibody (left) compared to Alexa Fluor Plus 555 secondary antibody (right, goat anti-mouse IgG secondary antibody, Cat. No. A32727) in immunofluorescent analysis of β-III tubulin in E18 Sprague Dawley primary cortical neuronal cells. Nuclei were stained with Hoechst 33342 stain (Cat. No. H3570). The image contains overlay of neuronal tubulin (orange) and nuclei (blue). Images were taken on a confocal microscope at 40x magnification.

Product list: Alexa Fluor Plus 555 Secondary Antibodies
Goat anti-rabbit A32732
Goat anti-mouse A32727
Goat anti-chicken A32932
Donkey anti-goat A32816
Donkey anti-rabbit A32794
Donkey anti-mouse A32773
Secondary antibody conjugates of Alexa Fluor 594 vs. Alexa Fluor Plus 594
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Alexa Fluor Plus 594 allows you to see greater detail compared to traditional Alexa Fluor 594 conjugates. Traditional Alexa Fluor 594 secondary antibody (left) compared to Alexa Fluor Plus 594 secondary antibody (right, donkey anti-mouse IgG secondary antibody (Cat. No. A32744). Nuclei were stained with Hoechst 33342 stain (Cat. No. H3570) in immunofluorescent analysis of tubulin in U2OS cells. The image contains overlay of tubulin (red) and nuclei (blue). Images were taken on an EVOS FL Auto 2 Imaging System (Cat. No. AMAFD2000) with an Olympus 20X Super Apochromat objective (Cat. No. AMEP4734) at 20X magnification.

Product list: Alexa Fluor Plus 594 Secondary Antibodies
Goat anti-rabbit A32740
Goat anti-mouse A32742
Goat anti-chicken A32759
Donkey anti-goat A32758
Donkey anti-rabbit A32754
Donkey anti-mouse A32744
Secondary antibody conjugates of Alexa Fluor 647 vs. Alexa Fluor Plus 647
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Alexa Fluor Plus 647 allows you to see greater detail compared to traditional Alexa Fluor 647 conjugates. Traditional Alexa Fluor 647 secondary antibody (left) compared to Alexa Fluor Plus 647 secondary antibody (right, goat anti-mouse IgG secondary antibody, Cat. No. A32728) in immunofluorescent analysis of β-III tubulin in E18 Sprague Dawley primary cortical neuronal cells. Nuclei were stained with Hoechst 33342 stain (Cat. No. H3570). The image contains overlay of neuronal tubulin (pink) and nuclei (blue). Images were taken on a confocal microscope at 40x magnification.

Product list: Alexa Fluor Plus 647 Secondary Antibodies
Goat anti-rabbit A32733
Goat anti-mouse A32728
Goat anti-chicken A32933
Donkey anti-goat A32849
Donkey anti-rabbit A32795
Donkey anti-mouse A32787
Secondary antibody conjugates of Alexa Fluor 680 vs. Alexa Fluor Plus 680
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Alexa Fluor Plus 680 allows you to see greater detail compared to traditional Alexa Fluor 680 conjugates. Traditional Alexa Fluor 680 secondary antibody (left) compared to Alexa Fluor Plus 680 secondary antibody (right, goat anti-chicken IgY secondary antibody, Cat. No. A32934) in Immunofluorescent analysis of tubulin in A549 cells. Nuclei were stained with Hoechst 33342 stain (Cat. No. H3570). The image contains overlay of tubulin (purple) and nuclei (blue). Images were taken on an EVOS FL Auto 2 Imaging System (Cat. No. AMAFD2000) with an Olympus 20X Super Apochromat objective (Cat. No. AMEP4734) at 20X magnification.

Product list: Alexa Fluor Plus 680 Secondary Antibodies
Goat anti-rabbit A32734
Goat anti-mouse A32729
Goat anti-chicken A32934
Donkey anti-goat A32860
Donkey anti-rabbit A32802
Donkey anti-mouse A32788
 Secondary antibody conjugates of Alexa Fluor Plus 800 goat anti-rabbit vs. competitor
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Alexa Fluor Plus 800 shows more bands at lower dilutions, providing greater sensitivity and range of detection compared to traditional 800 near-IR conjugates. Competitor, 800 near-IR secondary antibody (bottom) compared to Alexa Fluor Plus 800 secondary antibody (top, goat anti-rabbit IgG secondary antibody, Cat. No. A32735) in a western blot analysis of heat shock protein 90 (Hsp90) and histone deacetylase 1 (HDAC1).

Product list: Alexa Fluor Plus 800 Secondary Antibodies
Goat anti-rabbit A32735
Goat anti-mouse A32730
Goat anti-chicken A32935
Donkey anti-goat A32930
Donkey anti-rabbit A32808
Donkey anti-mouse A32789

Invitrogen Alexa Fluor Plus Secondary Antibody products

  Alexa Fluor Plus 488 Alexa Fluor Plus 555 Alexa Fluor Plus 594 Alexa Fluor Plus 647 Alexa Fluor Plus 680 Alexa Fluor Plus 800
Goat anti-rabbit A32731 A32732 A32740 A32733 A32734 A32735
Goat anti-mouse A32723 A32727 A32742 A32728 A32729 A32730
Goat anti-chicken A32931 A32932 A32759 A32933 A32934 A32935
Donkey anti-goat A32814 A32816 A32758 A32849 A32860 A32930
Donkey anti-rabbit A32790 A32794 A32754 A32795 A32802 A32808
Donkey anti-mouse A32766 A32773 A32744 A32787 A32788 A32789

See real scientists’ reactions to the Alexa Fluor Plus images

Alexa Fluor Plus secondary antibody information & conjugates

Use
  • Designed to help detect and visualize low-abundance targets in rare or precious samples
Applications
Product attributes
  • Higher signal-to-noise ratio than traditional Alexa Fluor secondary antibodies
    • Up to 4.2 times higher signal to noise in immunofluorescence (IF)/ Immunocytochemistry (ICC) imaging
    • Up to 5.8 times higher signal to noise in fluorescent western blotting (WB)
  • Lower cross-reactivity compared to traditional Alexa Fluor secondary antibodies

Fluorophores

Targets

  • Goat anti-rabbit
  • Goat anti-mouse
  • Goat anti-chicken
  • Donkey anti-goat
  • Donkey anti-rabbit
  • Donkey anti-mouse  
Mountant compatibility
  • Most standard mountants

Recommended:

Fixative compatibility

  • Most standard fixatives
Recommended:
Organelle staining compatibility
  • Most standard organelle-specific antibodies and stains
Recommended:
Alexa Fluor Plus new packaging

New packaging helps save time and space

  • Storage made easier—the new vials have flagless labels. No more struggling to wrap the flag around the vial to fit it into racks or centrifuges.
  • Identifying made easier—vials have colored caps corresponding to the Alexa Fluor Plus dye of the secondary antibody conjugate. If you are looking for an Alexa Fluor Plus 488 antibody, just look for the green cap.
  • Easier to see how much antibody you have left—vials are clear so you can easily see the volume remaining. No need to pipette from an opaque or solid-colored vial just to estimate how much antibody remains.