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Alexa Fluor Secondary Antibodies
Browse our comprehensive portfolio of Invitrogen Alexa Fluor and Alexa Fluor Plus secondary antibodies for fluorescent detection of primary antibodies in a wide range of applications, such as cell imaging, flow cytometry and western blotting.
- Alexa Fluor Plus secondary antibodies—7 different dyes
- Alexa Fluor secondary antibodies—18 different dyes
- Alexa Fluor tandem dye-conjugated secondary antibodies
Invitrogen Alexa Fluor protein and antibody conjugates produce fluorescence outputs that exceed those of other spectrally similar fluorophore conjugates, and do not photobleach as easily as classic fluorescent secondary antibodies. With more than 30,000 publications, the Alexa Fluor dye family has been successfully used over the last three decades of fluorescence imaging.
Alexa Fluor Plus Secondary Antibodies
Need to detect and visualize low-abundance targets in rare or precious samples?
Compare to traditional Alexa Fluor secondary antibodies:
- Higher signal-to-noise ratio
- Lower cross-reactivity
Alexa Fluor Secondary Antibody Product Information
| Use | Designed for use in a variety of cell analysis and protein analysis applications including imaging and fluorescent western blotting. | |||
| Applications | Cell Imaging
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| Product attributes | Cell Imaging
Multiplex, fluorescent western blotting
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Targets available |
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| Fluorophores available |
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| Mountant compatibility |
Recommended:
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Fixative compatibility |
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| Organelle staining compatibility |
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Alexa Fluor ReadyProbes Secondary Antibodies
ReadyProbes secondary antibodies are conjugated with bright and photostable Alexa Fluor 488 and Alexa Fluor 594 dyes to offer convenient and robust labeling. These Alexa Fluor secondary antibodies recognize IgG heavy chains and all classes of immunoglobulin light chains from either mouse or rabbit. Like other ReadyProbes reagents, these secondary antibodies are provided in a convenient dropper bottle format. No pipetting or dilutions are required. Just add 2 drops per milliliter of buffer to stain your cells, and follow the icon-based protocol.
CAKI cell peroxisomes labeled using a rabbit anti-PMP70 antibody, followed by detection with Alexa Fluor 488 Goat Anti–Rabbit IgG Antibody ReadyProbes Reagent.
Alexa Fluor ReadyProbes Secondary Antibody products
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