Secondary antibody conjugates of classical fluorescent dyes like fluorescein (FITC), tetramethylrhodamine (TRITC), Rhodamine Red, Cy3, Cy5, and Texas Red, now have Alexa Fluor conjugate alternatives, which provide better brightness and photostability. Nevertheless, traditional dyes are still appropriate when one must maintain a proven process or requires a more affordable option. Additionally, there may be applications where these dyes are especially useful, such as taking advantage of the high rate of photobleaching or pH sensitivity of fluorescein, or when trying to replicate historic experiments. We offer a broad range of classical dyes conjugated to a wide array of secondary antibodies to help you find the right antibodies for your experiments. 


How to find a secondary antibody

Step 1: Search for the target species of interest (e.g., “anti-mouse” or “goat anti-rabbit” or “alexa fluor 488”).
Step 2: Narrow results by host species, conjugate, application, and other criteria using side filters.


Quick links to products

Classical fluorophore secondary antibody conjugate products

Classical fluorophore secondary antibody conjugates recommendation table

Classical conjugate Dye and conjugate characteristics
FITC
  • Bright green fluorophore
  • 488 nm laser line
  • Low photostability
  • Sensitive to changes in pH
  • The most common fluorescent reagent for biological research
TRITC
  • Bright orange fluorophore
  • 488/532 laser line
  • Typically used for cellular imagining applications
Texas Red
  • Bright red fluorophore
  • 561/594 laser line
  • Susceptible to hydrolysis
  • Typically used for cellular imagining applications
R-PE
  • Bright red-orange fluorophore
  • 488-561 laser line
  • Low photostability
  • Typically used for flow cytometry, ELISA, and other applications that require high sensitivity
APC
  • Bright far-red fluorophore
  • 594-633 laser line
  • Typically used for flow cytometry, ELISA, and other applications that require high sensitivity
Pacific Dyes
  • Available in blue, green, and orange fluorophores
  • Allows three-color analysis
  • Strongly fluorescent even at neutral pH
  • Low photostability
  • Typically used for flow cytometry

Alexa Fluor Secondary Antibodies

Alexa Fluor secondary antibodies exhibit superior brightness and photostability, outperforming conventional fluorescent secondary antibodies. Select from 18 different individual Alexa Fluor dyes and 4 Alexa Fluor tandem dyes as secondary antibody conjugates.

Compare secondary antibody fluorophore technologies