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When selecting a secondary antibody for your application, use our instrument compatibility table to guide you to the right Invitrogen Alexa Fluor secondary antibody to fit your research needs. Whether your application is cell imaging using microscopes, microplate readers, high content screening, or fluorescent western blotting, we have options.
| Alexa Fluor conjugate | Ex/Em (nm) | Standard microscopy | EVOS Cell Imaging Systems | Secondary Antibodies |
|---|---|---|---|---|
| Recommended, standard filter sets | See recommended Light Cubes and Alexa Fluor reagents for the EVOS Cell Imaging Systems | Search all Alexa Fluor secondary antibodies | ||
| Alexa Fluor 350 | 346/442 | DAPI | DAPI Light Cube recommended | See Alexa Fluor 350 secondary antibodies |
| Alexa Fluor 405 Alexa Fluor Plus 405 | 401/421 | DAPI | DAPI Light Cube recommended | See Alexa Fluor 405 secondary antibodies |
| Alexa Fluor 430 | 425/530 | Custom filter | GFP Light Cube compatible | See Alexa Fluor 430 secondary antibodies |
| Alexa Fluor 488 Alexa Fluor Plus 488 | 490/525 | FITC | GFP Light Cube recommended | See all Alexa Fluor 488 secondary antibodies |
| Alexa Fluor 532 | 532/554 | TRITC | RFP Light Cube compatible | See all Alexa Fluor 532 secondary antibodies |
| Alexa Fluor 546 | 556/573 | TRITC | RFP Light Cube compatible | See all Alexa Fluor 546 secondary antibodies |
| Alexa Fluor 555 Alexa Fluor Plus 555 | 555/580 | TRITC | RFP Light Cube recommended | See all Alexa Fluor 555 secondary antibodies |
| Alexa Fluor 568 | 578/603 | Texas Red | Texas Red Light Cube compatible | See all Alexa Fluor 568 secondary antibodies |
| Alexa Fluor 594 Alexa Fluor Plus 594 | 590/617 | Texas Red | Texas Red Light Cube recommended | See Alexa Fluor 594 secondary antibodies |
| Alexa Fluor 633 | 633/645 | Cy5 | Cy5 Light Cube compatible | See all Alexa Fluor 633 secondary antibodies |
| Alexa Fluor 635 | 621/639 | Cy5 | Cy5 Light Cube compatible | See all Alexa Fluor 635 secondary antibodies |
| Alexa Fluor 647 Alexa Fluor Plus 647 | 650/665 | Cy5 | Cy5 Light Cube compatible | See all Alexa Fluor 647 secondary antibodies |
| Alexa Fluor 660 | 660/689 | Custom filter | Cy5 Light Cube recommended | See all Alexa Fluor 660 secondary antibodies |
| Alexa Fluor 680 Alexa Fluor Plus 680 | 679/702 | Cy5.5 | Cy5 or Cy5.5 Light Cube compatible | See all Alexa Fluor 680 secondary antibodies |
| Alexa Fluor 700 | 702/724 | Custom filter | Cy5.5 Light Cube compatible | See all Alexa Fluor 700 secondary antibodies |
| Alexa Fluor 750 | 749/775 | Cy7 | Cy7 Light Cube recommended | See all Alexa Fluor 750 secondary antibodies |
| Alexa Fluor Plus 800 | 777/794 | Cy7 | Cy7 Light Cube compatible | See all Alexa Fluor 800 secondary antibodies |
*Compatible with all Alexa Fluor dyes, choose any wavelength you like 200-1000 nm
| Alexa Fluor conjugate | Ex/Em (nm) | CellInsight CX5 High-Content Screening (HCS) Platform | CellInsight CX7 High-Content Screening (HCS) Platform | CellInsight CX7 LZR High-Content Screening (HCS) Platform | Alexa Fluor Secondary Antibodies |
|---|---|---|---|---|---|
| See the CX5 Platform Reagent Selection Guide | See the CX7 Platform Reagent Selection Guide | See the CX7 LZR Platform Reagent Selection Guide | Search all Alexa Fluor secondary antibodies | ||
| Alexa Fluor 350 | 346/442 | CX5 Platform recommended | CX7 Platform recommended | CX7 LZR Platform recommended | See Alexa Fluor 350 secondary antibodies |
| Alexa Fluor 405 Alexa Fluor Plus 405 | 401/421 | CX7 Platform recommended | See Alexa Fluor 405 secondary antibodies | ||
| Alexa Fluor 488 Alexa Fluor Plus 488 | 490/525 | CX5 Platform recommended | CX7 Platform recommended | CX7 LZR Platform recommended | See all Alexa Fluor 488 secondary antibodies |
| Alexa Fluor 532 | 532/554 | CX7 Platform recommended | CX7 LZR Platform recommended | See all Alexa Fluor 532 secondary antibodies | |
| Alexa Fluor 555 Alexa Fluor Plus 555 | 555/580 | CX5 Platform recommended | CX7 Platform recommended | CX7 LZR Platform recommended | See all Alexa Fluor 555 secondary antibodies |
| Alexa Fluor 594 Alexa Fluor Plus 594 | 590/617 | CX5 Platform recommended | CX7 Platform recommended | CX7 LZR Platform recommended | See Alexa Fluor 594 secondary antibodies |
| Alexa Fluor 647 Alexa Fluor Plus 647 | 650/665 | CX5 Platform recommended | CX7 Platform recommended | CX7 LZR Platform recommended | See all Alexa Fluor 647 secondary antibodies |
| Alexa Fluor 680 Alexa Fluor Plus 680 | 679/702 | CX5 Platform recommended | CX7 Platform recommended | CX7 LZR Platform recommended | See all Alexa Fluor 680 secondary antibodies |
| Alexa Fluor 750 | 749/775 | CX5 Platform recommended | CX7 Platform recommended | CX7 LZR Platform recommended | See all Alexa Fluor 750 secondary antibodies |
| Alexa Fluor 790 | 784/814 | CX7 LZR Platform recommended | See all Alexa Fluor 790 secondary antibodies | ||
| Alexa Fluor Plus 800 | 777/794 | CX5 Platform recommended | CX7 Platform recommended | CX7 LZR Platform recommended | See all Alexa Fluor 800 secondary antibodies |
| Alexa Fluor conjugate | Ex/Em (nm) | iBright Western Blot Imaging Systems | Alexa Fluor Secondary Antibodies |
|---|---|---|---|
| See compatible reagents for the iBright Western Blot Imaging Systems | Search all Alexa Fluor secondary antibodies | ||
| Alexa Fluor 488 Alexa Fluor Plus 488 | 490/525 | iBright recommended | See all Alexa Fluor 488 secondary antibodies |
| Alexa Fluor 546 | 556/573 | iBright recommended | See all Alexa Fluor 546 secondary antibodies |
| Alexa Fluor 555 Alexa Fluor Plus 555 | 555/580 | iBright recommended | See all Alexa Fluor 555 secondary antibodies |
| Alexa Fluor 635 | 621/639 | iBright recommended | See all Alexa Fluor 635 secondary antibodies |
| Alexa Fluor 647 Alexa Fluor Plus 647 | 650/665 | iBright recommended | See all Alexa Fluor 647 secondary antibodies |
| Alexa Fluor 680 Alexa Fluor Plus 680 | 679/702 | iBright recommended | See all Alexa Fluor 680 secondary antibodies |
| Alexa Fluor 790 | 784/814 | iBright recommended | See all Alexa Fluor 790 secondary antibodies |
| Alexa Fluor Plus 800 | 777/794 | iBright recommended | See all Alexa Fluor 800 secondary antibodies |
| Alexa Fluor conjugate | Ex/Em (nm) | Standard microscopy | EVOS Cell Imaging Systems | Secondary Antibodies |
|---|---|---|---|---|
| Recommended, standard filter sets | See recommended Light Cubes and Alexa Fluor reagents for the EVOS Cell Imaging Systems | Search all Alexa Fluor secondary antibodies | ||
| Alexa Fluor 350 | 346/442 | DAPI | DAPI Light Cube recommended | See Alexa Fluor 350 secondary antibodies |
| Alexa Fluor 405 Alexa Fluor Plus 405 | 401/421 | DAPI | DAPI Light Cube recommended | See Alexa Fluor 405 secondary antibodies |
| Alexa Fluor 430 | 425/530 | Custom filter | GFP Light Cube compatible | See Alexa Fluor 430 secondary antibodies |
| Alexa Fluor 488 Alexa Fluor Plus 488 | 490/525 | FITC | GFP Light Cube recommended | See all Alexa Fluor 488 secondary antibodies |
| Alexa Fluor 532 | 532/554 | TRITC | RFP Light Cube compatible | See all Alexa Fluor 532 secondary antibodies |
| Alexa Fluor 546 | 556/573 | TRITC | RFP Light Cube compatible | See all Alexa Fluor 546 secondary antibodies |
| Alexa Fluor 555 Alexa Fluor Plus 555 | 555/580 | TRITC | RFP Light Cube recommended | See all Alexa Fluor 555 secondary antibodies |
| Alexa Fluor 568 | 578/603 | Texas Red | Texas Red Light Cube compatible | See all Alexa Fluor 568 secondary antibodies |
| Alexa Fluor 594 Alexa Fluor Plus 594 | 590/617 | Texas Red | Texas Red Light Cube recommended | See Alexa Fluor 594 secondary antibodies |
| Alexa Fluor 633 | 633/645 | Cy5 | Cy5 Light Cube compatible | See all Alexa Fluor 633 secondary antibodies |
| Alexa Fluor 635 | 621/639 | Cy5 | Cy5 Light Cube compatible | See all Alexa Fluor 635 secondary antibodies |
| Alexa Fluor 647 Alexa Fluor Plus 647 | 650/665 | Cy5 | Cy5 Light Cube compatible | See all Alexa Fluor 647 secondary antibodies |
| Alexa Fluor 660 | 660/689 | Custom filter | Cy5 Light Cube recommended | See all Alexa Fluor 660 secondary antibodies |
| Alexa Fluor 680 Alexa Fluor Plus 680 | 679/702 | Cy5.5 | Cy5 or Cy5.5 Light Cube compatible | See all Alexa Fluor 680 secondary antibodies |
| Alexa Fluor 700 | 702/724 | Custom filter | Cy5.5 Light Cube compatible | See all Alexa Fluor 700 secondary antibodies |
| Alexa Fluor 750 | 749/775 | Cy7 | Cy7 Light Cube recommended | See all Alexa Fluor 750 secondary antibodies |
| Alexa Fluor Plus 800 | 777/794 | Cy7 | Cy7 Light Cube compatible | See all Alexa Fluor 800 secondary antibodies |
*Compatible with all Alexa Fluor dyes, choose any wavelength you like 200-1000 nm
| Alexa Fluor conjugate | Ex/Em (nm) | CellInsight CX5 High-Content Screening (HCS) Platform | CellInsight CX7 High-Content Screening (HCS) Platform | CellInsight CX7 LZR High-Content Screening (HCS) Platform | Alexa Fluor Secondary Antibodies |
|---|---|---|---|---|---|
| See the CX5 Platform Reagent Selection Guide | See the CX7 Platform Reagent Selection Guide | See the CX7 LZR Platform Reagent Selection Guide | Search all Alexa Fluor secondary antibodies | ||
| Alexa Fluor 350 | 346/442 | CX5 Platform recommended | CX7 Platform recommended | CX7 LZR Platform recommended | See Alexa Fluor 350 secondary antibodies |
| Alexa Fluor 405 Alexa Fluor Plus 405 | 401/421 | CX7 Platform recommended | See Alexa Fluor 405 secondary antibodies | ||
| Alexa Fluor 488 Alexa Fluor Plus 488 | 490/525 | CX5 Platform recommended | CX7 Platform recommended | CX7 LZR Platform recommended | See all Alexa Fluor 488 secondary antibodies |
| Alexa Fluor 532 | 532/554 | CX7 Platform recommended | CX7 LZR Platform recommended | See all Alexa Fluor 532 secondary antibodies | |
| Alexa Fluor 555 Alexa Fluor Plus 555 | 555/580 | CX5 Platform recommended | CX7 Platform recommended | CX7 LZR Platform recommended | See all Alexa Fluor 555 secondary antibodies |
| Alexa Fluor 594 Alexa Fluor Plus 594 | 590/617 | CX5 Platform recommended | CX7 Platform recommended | CX7 LZR Platform recommended | See Alexa Fluor 594 secondary antibodies |
| Alexa Fluor 647 Alexa Fluor Plus 647 | 650/665 | CX5 Platform recommended | CX7 Platform recommended | CX7 LZR Platform recommended | See all Alexa Fluor 647 secondary antibodies |
| Alexa Fluor 680 Alexa Fluor Plus 680 | 679/702 | CX5 Platform recommended | CX7 Platform recommended | CX7 LZR Platform recommended | See all Alexa Fluor 680 secondary antibodies |
| Alexa Fluor 750 | 749/775 | CX5 Platform recommended | CX7 Platform recommended | CX7 LZR Platform recommended | See all Alexa Fluor 750 secondary antibodies |
| Alexa Fluor 790 | 784/814 | CX7 LZR Platform recommended | See all Alexa Fluor 790 secondary antibodies | ||
| Alexa Fluor Plus 800 | 777/794 | CX5 Platform recommended | CX7 Platform recommended | CX7 LZR Platform recommended | See all Alexa Fluor 800 secondary antibodies |
| Alexa Fluor conjugate | Ex/Em (nm) | iBright Western Blot Imaging Systems | Alexa Fluor Secondary Antibodies |
|---|---|---|---|
| See compatible reagents for the iBright Western Blot Imaging Systems | Search all Alexa Fluor secondary antibodies | ||
| Alexa Fluor 488 Alexa Fluor Plus 488 | 490/525 | iBright recommended | See all Alexa Fluor 488 secondary antibodies |
| Alexa Fluor 546 | 556/573 | iBright recommended | See all Alexa Fluor 546 secondary antibodies |
| Alexa Fluor 555 Alexa Fluor Plus 555 | 555/580 | iBright recommended | See all Alexa Fluor 555 secondary antibodies |
| Alexa Fluor 635 | 621/639 | iBright recommended | See all Alexa Fluor 635 secondary antibodies |
| Alexa Fluor 647 Alexa Fluor Plus 647 | 650/665 | iBright recommended | See all Alexa Fluor 647 secondary antibodies |
| Alexa Fluor 680 Alexa Fluor Plus 680 | 679/702 | iBright recommended | See all Alexa Fluor 680 secondary antibodies |
| Alexa Fluor 790 | 784/814 | iBright recommended | See all Alexa Fluor 790 secondary antibodies |
| Alexa Fluor Plus 800 | 777/794 | iBright recommended | See all Alexa Fluor 800 secondary antibodies |
After using the tables above to determine the Alexa Fluor dye that would fit your application, visit the Fluorophore Selection Guide and click on the corresponding Alexa Fluor dye page of interest. Each dye page provides information about the various products available for use with that Alexa Fluor dye.