When selecting a secondary antibody for your application, use our instrument compatibility table to guide you to the right Invitrogen Alexa Fluor secondary antibody to fit your research needs. Whether your application is cell imaging using microscopes, microplate readers, high content screening, or fluorescent western blotting, we have options.

Alexa Fluor secondary antibody instrument compatibility tables

Alexa Fluor conjugate Ex/Em (nm) Standard microscopy EVOS Cell Imaging Systems Secondary Antibodies
    Recommended, standard filter sets See recommended Light Cubes and Alexa Fluor reagents for the EVOS Cell Imaging Systems Search all Alexa Fluor secondary antibodies
Alexa Fluor 350 346/442 DAPI DAPI Light Cube recommended See Alexa Fluor 350 secondary antibodies
Alexa Fluor 405 401/421 DAPI DAPI Light Cube recommended See Alexa Fluor 405 secondary antibodies
Alexa Fluor 430 425/530 Custom filter GFP Light Cube compatible See Alexa Fluor 430 secondary antibodies
Alexa Fluor 488
Alexa Fluor Plus 488
490/525 FITC GFP Light Cube recommended See all Alexa Fluor 488 secondary antibodies
Alexa Fluor 532 532/554 TRITC RFP Light Cube compatible See all Alexa Fluor 532 secondary antibodies
Alexa Fluor 546 556/573 TRITC RFP Light Cube compatible See all Alexa Fluor 546 secondary antibodies
Alexa Fluor 555
Alexa Fluor Plus 555
555/580 TRITC RFP Light Cube recommended See all Alexa Fluor 555 secondary antibodies
Alexa Fluor 568 578/603 Texas Red Texas Red Light Cube compatible See all Alexa Fluor 568 secondary antibodies
Alexa Fluor 594
Alexa Fluor Plus 594
590/617 Texas Red Texas Red Light Cube recommended See Alexa Fluor 594 secondary antibodies
Alexa Fluor 633 633/645 Cy5 Cy5 Light Cube compatible See all Alexa Fluor 633 secondary antibodies
Alexa Fluor 635 621/639 Cy5 Cy5 Light Cube compatible See all Alexa Fluor 635 secondary antibodies
Alexa Fluor 647
Alexa Fluor Plus 647
650/665 Cy5 Cy5 Light Cube compatible See all Alexa Fluor 647 secondary antibodies
Alexa Fluor 660 660/689 Custom filter Cy5 Light Cube recommended See all Alexa Fluor 660 secondary antibodies
Alexa Fluor 680
Alexa Fluor Plus 680
679/702 Cy5.5 Cy5 or Cy5.5 Light Cube compatible See all Alexa Fluor 680 secondary antibodies
Alexa Fluor 700 702/724 Custom filter Cy5.5 Light Cube compatible See all Alexa Fluor 700 secondary antibodies
Alexa Fluor 750 749/775 Cy7 Cy7 Light Cube recommended See all Alexa Fluor 750 secondary antibodies
Alexa Fluor Plus 800 777/794 Cy7 Cy7 Light Cube compatible See all Alexa Fluor 800 secondary antibodies
Alexa Fluor conjugate Ex/Em (nm) Varioskan LUX Multimode Reader* Fluoroskan Microplate Fluorometer Fluoroskan FL Microplate Fluorometer Alexa Fluor Secondary Antibodies
      See recommended filter pairs for various fluorescent dyes on the Fluoroskan and Fluoroskan FL Microplate Fluorometers Search all Alexa Fluor secondary antibodies
Alexa Fluor 350 346/442 Recommended Recommended Recommended See Alexa Fluor 350 secondary antibodies
Alexa Fluor 405 401/421 Recommended Recommended Recommended See Alexa Fluor 405 secondary antibodies
Alexa Fluor 430 425/530 Recommended Recommended Recommended See Alexa Fluor 430 secondary antibodies
Alexa Fluor 488
Alexa Fluor Plus 488
490/525 Recommended Recommended Recommended See all Alexa Fluor 488 secondary antibodies
Alexa Fluor 514 517/542 Recommended Recommended Recommended See all Alexa Fluor 514  secondary antibodies
Alexa Fluor 532 532/554 Recommended Recommended Recommended See all Alexa Fluor 532 secondary antibodies
Alexa Fluor 546 556/573 Recommended Recommended Recommended See all Alexa Fluor 546 secondary antibodies
Alexa Fluor 555
Alexa Fluor Plus 555
555/580 Recommended Recommended Recommended See all Alexa Fluor 555 secondary antibodies
Alexa Fluor 568 578/603 Recommended Recommended Recommended See all Alexa Fluor 568 secondary antibodies
Alexa Fluor 594
Alexa Fluor Plus 594
590/617 Recommended Recommended Recommended See Alexa Fluor 594 secondary antibodies
Alexa Fluor 633 633/645 Recommended Recommended Recommended See all Alexa Fluor 633 secondary antibodies
Alexa Fluor 635 621/639 Recommended Recommended Recommended See all Alexa Fluor 635 secondary antibodies
Alexa Fluor 647
Alexa Fluor Plus 647
650/665 Recommended Recommended   See all Alexa Fluor 647 secondary antibodies
Alexa Fluor 660 660/689 Recommended Recommended   See all Alexa Fluor 660 secondary antibodies
Alexa Fluor 680
Alexa Fluor Plus 680
679/702 Recommended Recommended   See all Alexa Fluor 680 secondary antibodies
Alexa Fluor 700 702/724 Recommended     See all Alexa Fluor 700 secondary antibodies
Alexa Fluor 750 749/775 Recommended     See all Alexa Fluor 750 secondary antibodies
Alexa Fluor 790 784/814 Recommended     See all Alexa Fluor 790 secondary antibodies
Alexa Fluor Plus 800 777/794 Recommended     See all Alexa Fluor 800 secondary antibodies

*Compatible with all Alexa Fluor dyes, choose any wavelength you like 200-1000 nm

Alexa Fluor conjugate Ex/Em (nm) CellInsight CX5 High-Content Screening (HCS) Platform CellInsight CX7 High-Content Screening (HCS) Platform CellInsight CX7 LZR High-Content Screening (HCS) Platform Alexa Fluor Secondary Antibodies
    See the CX5 Platform Reagent Selection Guide
See the CX7 Platform Reagent Selection Guide See the CX7 LZR Platform Reagent Selection Guide Search all Alexa Fluor secondary antibodies
Alexa Fluor 350 346/442 CX5 Platform recommended CX7 Platform recommended CX7 LZR Platform recommended See Alexa Fluor 350 secondary antibodies
Alexa Fluor 405 401/421   CX7 Platform recommended   See Alexa Fluor 405 secondary antibodies
Alexa Fluor 488
Alexa Fluor Plus 488
490/525 CX5 Platform recommended CX7 Platform recommended CX7 LZR Platform recommended See all Alexa Fluor 488 secondary antibodies
Alexa Fluor 532 532/554   CX7 Platform recommended CX7 LZR Platform recommended See all Alexa Fluor 532 secondary antibodies
Alexa Fluor 555
Alexa Fluor Plus 555
555/580 CX5 Platform recommended CX7 Platform recommended CX7 LZR Platform recommended See all Alexa Fluor 555 secondary antibodies
Alexa Fluor 594
Alexa Fluor Plus 594
590/617 CX5 Platform recommended CX7 Platform recommended CX7 LZR Platform recommended See Alexa Fluor 594 secondary antibodies
Alexa Fluor 647
Alexa Fluor Plus 647
650/665 CX5 Platform recommended CX7 Platform recommended CX7 LZR Platform recommended See all Alexa Fluor 647 secondary antibodies
Alexa Fluor 680
Alexa Fluor Plus 680
679/702 CX5 Platform recommended CX7 Platform recommended CX7 LZR Platform recommended See all Alexa Fluor 680 secondary antibodies
Alexa Fluor 750 749/775 CX5 Platform recommended CX7 Platform recommended CX7 LZR Platform recommended See all Alexa Fluor 750 secondary antibodies
Alexa Fluor 790 784/814     CX7 LZR Platform recommended See all Alexa Fluor 790 secondary antibodies
Alexa Fluor Plus 800 777/794 CX5 Platform recommended CX7 Platform recommended CX7 LZR Platform recommended See all Alexa Fluor 800 secondary antibodies
Alexa Fluor conjugate Ex/Em (nm) iBright Western Blot Imaging Systems Alexa Fluor Secondary Antibodies
    See compatible reagents for the iBright Western Blot Imaging Systems Search all Alexa Fluor secondary antibodies
Alexa Fluor 488
Alexa Fluor Plus 488
490/525 iBright recommended See all Alexa Fluor 488 secondary antibodies
Alexa Fluor 546 556/573 iBright recommended See all Alexa Fluor 546 secondary antibodies
Alexa Fluor 555
Alexa Fluor Plus 555
555/580 iBright recommended See all Alexa Fluor 555 secondary antibodies
Alexa Fluor 635 621/639 iBright recommended See all Alexa Fluor 635 secondary antibodies
Alexa Fluor 647
Alexa Fluor Plus 647
650/665 iBright recommended See all Alexa Fluor 647 secondary antibodies
Alexa Fluor 680
Alexa Fluor Plus 680
679/702 iBright recommended See all Alexa Fluor 680 secondary antibodies
Alexa Fluor 790 784/814 iBright recommended See all Alexa Fluor 790 secondary antibodies
Alexa Fluor Plus 800 777/794 iBright recommended See all Alexa Fluor 800 secondary antibodies
Alexa Fluor conjugate Ex/Em (nm) Standard microscopy EVOS Cell Imaging Systems Secondary Antibodies
    Recommended, standard filter sets See recommended Light Cubes and Alexa Fluor reagents for the EVOS Cell Imaging Systems Search all Alexa Fluor secondary antibodies
Alexa Fluor 350 346/442 DAPI DAPI Light Cube recommended See Alexa Fluor 350 secondary antibodies
Alexa Fluor 405 401/421 DAPI DAPI Light Cube recommended See Alexa Fluor 405 secondary antibodies
Alexa Fluor 430 425/530 Custom filter GFP Light Cube compatible See Alexa Fluor 430 secondary antibodies
Alexa Fluor 488
Alexa Fluor Plus 488
490/525 FITC GFP Light Cube recommended See all Alexa Fluor 488 secondary antibodies
Alexa Fluor 532 532/554 TRITC RFP Light Cube compatible See all Alexa Fluor 532 secondary antibodies
Alexa Fluor 546 556/573 TRITC RFP Light Cube compatible See all Alexa Fluor 546 secondary antibodies
Alexa Fluor 555
Alexa Fluor Plus 555
555/580 TRITC RFP Light Cube recommended See all Alexa Fluor 555 secondary antibodies
Alexa Fluor 568 578/603 Texas Red Texas Red Light Cube compatible See all Alexa Fluor 568 secondary antibodies
Alexa Fluor 594
Alexa Fluor Plus 594
590/617 Texas Red Texas Red Light Cube recommended See Alexa Fluor 594 secondary antibodies
Alexa Fluor 633 633/645 Cy5 Cy5 Light Cube compatible See all Alexa Fluor 633 secondary antibodies
Alexa Fluor 635 621/639 Cy5 Cy5 Light Cube compatible See all Alexa Fluor 635 secondary antibodies
Alexa Fluor 647
Alexa Fluor Plus 647
650/665 Cy5 Cy5 Light Cube compatible See all Alexa Fluor 647 secondary antibodies
Alexa Fluor 660 660/689 Custom filter Cy5 Light Cube recommended See all Alexa Fluor 660 secondary antibodies
Alexa Fluor 680
Alexa Fluor Plus 680
679/702 Cy5.5 Cy5 or Cy5.5 Light Cube compatible See all Alexa Fluor 680 secondary antibodies
Alexa Fluor 700 702/724 Custom filter Cy5.5 Light Cube compatible See all Alexa Fluor 700 secondary antibodies
Alexa Fluor 750 749/775 Cy7 Cy7 Light Cube recommended See all Alexa Fluor 750 secondary antibodies
Alexa Fluor Plus 800 777/794 Cy7 Cy7 Light Cube compatible See all Alexa Fluor 800 secondary antibodies
Alexa Fluor conjugate Ex/Em (nm) Varioskan LUX Multimode Reader* Fluoroskan Microplate Fluorometer Fluoroskan FL Microplate Fluorometer Alexa Fluor Secondary Antibodies
      See recommended filter pairs for various fluorescent dyes on the Fluoroskan and Fluoroskan FL Microplate Fluorometers Search all Alexa Fluor secondary antibodies
Alexa Fluor 350 346/442 Recommended Recommended Recommended See Alexa Fluor 350 secondary antibodies
Alexa Fluor 405 401/421 Recommended Recommended Recommended See Alexa Fluor 405 secondary antibodies
Alexa Fluor 430 425/530 Recommended Recommended Recommended See Alexa Fluor 430 secondary antibodies
Alexa Fluor 488
Alexa Fluor Plus 488
490/525 Recommended Recommended Recommended See all Alexa Fluor 488 secondary antibodies
Alexa Fluor 514 517/542 Recommended Recommended Recommended See all Alexa Fluor 514  secondary antibodies
Alexa Fluor 532 532/554 Recommended Recommended Recommended See all Alexa Fluor 532 secondary antibodies
Alexa Fluor 546 556/573 Recommended Recommended Recommended See all Alexa Fluor 546 secondary antibodies
Alexa Fluor 555
Alexa Fluor Plus 555
555/580 Recommended Recommended Recommended See all Alexa Fluor 555 secondary antibodies
Alexa Fluor 568 578/603 Recommended Recommended Recommended See all Alexa Fluor 568 secondary antibodies
Alexa Fluor 594
Alexa Fluor Plus 594
590/617 Recommended Recommended Recommended See Alexa Fluor 594 secondary antibodies
Alexa Fluor 633 633/645 Recommended Recommended Recommended See all Alexa Fluor 633 secondary antibodies
Alexa Fluor 635 621/639 Recommended Recommended Recommended See all Alexa Fluor 635 secondary antibodies
Alexa Fluor 647
Alexa Fluor Plus 647
650/665 Recommended Recommended   See all Alexa Fluor 647 secondary antibodies
Alexa Fluor 660 660/689 Recommended Recommended   See all Alexa Fluor 660 secondary antibodies
Alexa Fluor 680
Alexa Fluor Plus 680
679/702 Recommended Recommended   See all Alexa Fluor 680 secondary antibodies
Alexa Fluor 700 702/724 Recommended     See all Alexa Fluor 700 secondary antibodies
Alexa Fluor 750 749/775 Recommended     See all Alexa Fluor 750 secondary antibodies
Alexa Fluor 790 784/814 Recommended     See all Alexa Fluor 790 secondary antibodies
Alexa Fluor Plus 800 777/794 Recommended     See all Alexa Fluor 800 secondary antibodies

*Compatible with all Alexa Fluor dyes, choose any wavelength you like 200-1000 nm

Alexa Fluor conjugate Ex/Em (nm) CellInsight CX5 High-Content Screening (HCS) Platform CellInsight CX7 High-Content Screening (HCS) Platform CellInsight CX7 LZR High-Content Screening (HCS) Platform Alexa Fluor Secondary Antibodies
    See the CX5 Platform Reagent Selection Guide
See the CX7 Platform Reagent Selection Guide See the CX7 LZR Platform Reagent Selection Guide Search all Alexa Fluor secondary antibodies
Alexa Fluor 350 346/442 CX5 Platform recommended CX7 Platform recommended CX7 LZR Platform recommended See Alexa Fluor 350 secondary antibodies
Alexa Fluor 405 401/421   CX7 Platform recommended   See Alexa Fluor 405 secondary antibodies
Alexa Fluor 488
Alexa Fluor Plus 488
490/525 CX5 Platform recommended CX7 Platform recommended CX7 LZR Platform recommended See all Alexa Fluor 488 secondary antibodies
Alexa Fluor 532 532/554   CX7 Platform recommended CX7 LZR Platform recommended See all Alexa Fluor 532 secondary antibodies
Alexa Fluor 555
Alexa Fluor Plus 555
555/580 CX5 Platform recommended CX7 Platform recommended CX7 LZR Platform recommended See all Alexa Fluor 555 secondary antibodies
Alexa Fluor 594
Alexa Fluor Plus 594
590/617 CX5 Platform recommended CX7 Platform recommended CX7 LZR Platform recommended See Alexa Fluor 594 secondary antibodies
Alexa Fluor 647
Alexa Fluor Plus 647
650/665 CX5 Platform recommended CX7 Platform recommended CX7 LZR Platform recommended See all Alexa Fluor 647 secondary antibodies
Alexa Fluor 680
Alexa Fluor Plus 680
679/702 CX5 Platform recommended CX7 Platform recommended CX7 LZR Platform recommended See all Alexa Fluor 680 secondary antibodies
Alexa Fluor 750 749/775 CX5 Platform recommended CX7 Platform recommended CX7 LZR Platform recommended See all Alexa Fluor 750 secondary antibodies
Alexa Fluor 790 784/814     CX7 LZR Platform recommended See all Alexa Fluor 790 secondary antibodies
Alexa Fluor Plus 800 777/794 CX5 Platform recommended CX7 Platform recommended CX7 LZR Platform recommended See all Alexa Fluor 800 secondary antibodies
Alexa Fluor conjugate Ex/Em (nm) iBright Western Blot Imaging Systems Alexa Fluor Secondary Antibodies
    See compatible reagents for the iBright Western Blot Imaging Systems Search all Alexa Fluor secondary antibodies
Alexa Fluor 488
Alexa Fluor Plus 488
490/525 iBright recommended See all Alexa Fluor 488 secondary antibodies
Alexa Fluor 546 556/573 iBright recommended See all Alexa Fluor 546 secondary antibodies
Alexa Fluor 555
Alexa Fluor Plus 555
555/580 iBright recommended See all Alexa Fluor 555 secondary antibodies
Alexa Fluor 635 621/639 iBright recommended See all Alexa Fluor 635 secondary antibodies
Alexa Fluor 647
Alexa Fluor Plus 647
650/665 iBright recommended See all Alexa Fluor 647 secondary antibodies
Alexa Fluor 680
Alexa Fluor Plus 680
679/702 iBright recommended See all Alexa Fluor 680 secondary antibodies
Alexa Fluor 790 784/814 iBright recommended See all Alexa Fluor 790 secondary antibodies
Alexa Fluor Plus 800 777/794 iBright recommended See all Alexa Fluor 800 secondary antibodies

Need information about Alexa Fluor conjugated primary antibodies or non-antibody Alexa Fluor products for your application?

After using the tables above to determine the Alexa Fluor dye that would fit your application, visit the Fluorophore Selection Guide and click on the corresponding Alexa Fluor dye page of interest. Each dye page provides information about the various products available for use with that Alexa Fluor dye.