What are HRP-conjugated secondary antibodies?

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Horseradish peroxidase (HRP) is a commonly used enzyme label with secondary antibodies to amplify the signal of detected targets. HRP catalyzes the oxidation of substrates in the presence of hydrogen peroxide and the result is either a colored precipitate or light. When chromogenic substrates come into contact with the enzyme a colored product precipitates which doesn’t require additional equipment to visualize. When a chemiluminescent substrate comes into contact with HRP light is emitted as a byproduct and must be captured by an imaging instrument.

Horseradish peroxidase (HRP)-conjugated secondary antibodies are commonly used for chemiluminescent, colorimetric, or fluorescent detection in applications, such as western blotting, ELISA, immuno-histochemistry, and others. For each application, HRP-conjugated secondary antibodies need substrates that can deliver high signal-to-noise ratios for the chosen detection method.

HRP-conjugated secondary antibodies by application

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HRP-conjugated secondary antibodies by popular target species

Our extensive portfolio of HRP-conjugated secondary antibodies offers many options for each target species. Search by popular target species to find the antibody that meets your needs.

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F(ab’)2 fragments result from digestion of a whole antibody with pepsin. These antibody fragments contain the strong divalent binding found in the variable regions but lack the Fc portion of the antibody.

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