Thermo Scientific™ Superclonal™ secondary antibodies represent a breakthrough in recombinant antibody technology designed to provide precise and accurate detection of mouse, rabbit and goat primary antibodies in a variety of applications. Each Superclonal secondary antibody is formulated and optimized to help achieve excellent results in ELISA, western blot, and cell imaging.
Anti-Rabbit Secondary Antibodies
Our anti-rabbit secondary antibodies are affinity-purified polyclonal or monoclonal antibodies with well-characterized specificity for rabbit immunoglobulin classes and fragments. They are available in host species including goat, donkey, mouse, chicken, and sheep, and in the following formats:
- Unlabeled antibodies
- Enzyme conjugates (alkaline phosphatase (AP) or horseradish peroxidase (HRP))
- Fluorescent conjugates (Alexa Fluor, DyLight, Qdot, Fluorescein (FITC), etc.)
Our secondary antibodies are available as whole IgG or the F(ab')2 fragment. Each of these antibodies is affinity purified using its target antigen (the primary antibody class and fragment) coupled to an agarose support. This affinity purification eliminates nonspecific antibodies, resulting in high specificity and low background signal. Conjugated antibodies are affinity purified before labeling.
Selected secondary antibodies have been further purified by adsorbing the antigen-purified antibody to an affinity column containing immobilized serum proteins or IgG of selected species. This additional processing minimizes antibody cross-reactivity to the serum proteins of those selected species. These antibodies are designated as Cross Adsorbed or Highly Cross Adsorbed, based on the amount of processing which has occurred.
How to find a secondary antibody
Step 1: Search for the target species of interest (e.g., “anti-mouse” or “goat anti-rabbit” or “alexa fluor 488”).
Step 2: Narrow results by host species, conjugate, application, and other criteria using side filters.
For Research Use Only. Not for use in diagnostic procedures.