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CaptureSelect™ biotinylated ligands are available for use in a range of analytical assays, and include everything from ligands binding to antibodies and derivatives thereof to plasma proteins and non-mAb biosimilars. The affinity ligand is chemically conjugated to biotin via an appropriate spacer that retains binding reactivity of the ligand when immobilized onto streptavidin-functionalized surfaces.

Purchase CaptureSelect™ biotinylated ligands

Applications for CaptureSelect™ biotinylated ligands include Capture ELISA, Western blot, Gyros’ Gyrolab®-based immunoassays, and label-free detection platforms such as those based on surface plasmon resonance (Biacore® and IBIS-MX96 systems) and bio-layer interferometry (ForteBio® Octet® systems). Detailed product information sheets provide protocols for setting up such assays.

ELISA
Western blot
Label-free
CaptureSelect™ biotinylated ligands
Antobodies and derivatives Characteristics

Biotin Anti-IgG-CH1

Binds human IgG and all Fab fragments thereof, through recognition of the CH1 domain on all four human IgG subclasses.

Biotin Anti-IgG-Fc (Human)

Binds human IgG, through recognition of the Fc region (CH3 domain) on all four human IgG subclasses.

Biotin Anti-IgG-Fc (Multi-species)

Binds IgG through recognition of the Fc region on IgG of multiple species. View product sheet for species coverage.

Biotin Anti-IgA

Binds human IgA, IgA-dimers and secretory IgA (sIgA), through recognition of the Fc region on both human IgA subclasses.

Biotin Anti-IgM

Binds human, mouse and rat IgM.

Biotin Anti-LC-lambda (Human)

Binds all human lambda light chains (LC), through recognition of the constant domain (CL-lambda) on any human antibody, Fab fragment or free light chain.

Biotin Anti-LC-kappa (Human)

Binds all human kappa light chains (LC), through recognition of the constant domain (CL-kappa) on any human antibody, Fab fragment or free light chain.   

Biotin Anti-LC-kappa (Murine)

Binds all mouse - and rat kappa light chains (LC), through recognition of the constant domain (CL-kappa) on any mouse and rat antibody, Fab fragment or free light chain.
Biotin Anti-LC-lambda (Mouse) Binds all mouse lambda light chains (LC), through recognition of the constant domain (CL-lambda) on any mouse antibody, Fab fragment or free light chain
Biotin Anti-LC-lambda (Rat) Binds all rat lambda light chains (LC), through recognition of the constant domain (CL-lambda) on any rat antibody, Fab fragment or free light chain

Biotin Anti-IgG3 (Human)

Binds human IgG3 without any cross-binding to other human IgG subclasses or antibody isotypes.

Biotin Anti-IgG4 (Human)

Binds human IgG4 without any cross-binding to other human IgG subclasses or antibody isotypes.

Biotin Human Fab-kappa Kinetics

High affinity capture of human IgG Fab fragments that possess a kappa light chain. Extreme low off-rates are achieved through avidity by targeting the CL-kappa domain of the Fab (light chain) and its IgG CH1 domain (heavy chain) simultaneously, thereby enabling Fab screening on target binding kinetics.

Biotin Human Fab-lambda Kinetics

High affinity capture of human IgG Fab fragments that possess a lambda light chain. Extreme low off-rates are achieved through avidity by targeting the CL-lambda domain of the Fab (light chain) and its IgG CH1 domain (heavy chain) simultaneously, thereby enabling Fab screening on target binding kinetics.

Biotin Anti-Free LC-kappa (Human)

Binds highly selective to free human kappa light chains and dimers thereof, through recognition of an epitope on the human CL-kappa domain that is masked in intact antibodies and Fab fragments, thereby providing a unique tool for specific detection and quantitation of free kappa light chains in (crude) antibody preps.

Biotin Human IgG-Fc PK

Binds highly selective to human IgG, through recognition of the Fc region on all four human IgG subclasses without cross-binding to mouse, rat, rhesus and cynomolgus monkey IgG, thereby making it extremely suitable for setting up pharmacokinetics (PK) assays.
Proteins Characteristics

Biotin Anti-C-tag

Binds the short C-tag sequence E-P-E-A (glutamic acid - proline - glutamic acid – alanine) when this affinity tag is fused at the C-terminus of a target protein.

Alexa Fluor™ 488 Anti-C-tag Binds the short C-tag sequence E-P-E-A (glutamic acid - proline - glutamic acid – alanine) when this affinity tag is fused at the C-terminus of a target protein.

Biotin Anti-FIX

Binds recombinant as well as plasma derived human Factor IX (FIX)

Biotin Anti-FVIII

Binds recombinant (full length and B-domain deleted) as well as plasma derived human Factor VIII (FVIII), through recognition of the FVIII light chain.

Biotin Anti-Gonadotropin Binds to the alpha chain of recombinant as well as natural sourced hCG (human chorionic gonadotropin), LH (luteinizing hormone), TSH (Thyroid-stimulating hormone) and FSH (follicle-stimulating hormone)
Biotin Anti-Human Albumin Binds recombinant as well as natural sourced human serum albumin (HSA) including HSA-fusion proteins
Biotin Anti-AAT Binds recombinant as well as natural sourced human α1-Antitrypsin (AAT)
Biotin Anti-C1-Inhibitor Binds recombinant as well as natural sourced human C1-Inhibitor (C1-inh, C1 esterase inhibitor)
Biotin Anti-Fibrinogen Binds recombinant as well as natural sourced human Fibrinogen (coagulation factor I, FI)
Biotin Anti-Transferrin Binds recombinant as well as natural sourced human Transferrin
Biotin Anti-α1-Acid Glycoprotein Binds recombinant as well as natural sourced human α1-Acid Glycoprotein (AGP) or Orosomucoid (ORM)
Biotin Anti-Haptoglobin Binds recombinant as well as natural sourced human Haptoglobin (Hp)
Biotin Anti-C3 Binds recombinant as well as natural sourced human Complement Component 3 (C3)
Biotin Anti-vWF Binds recombinant as well as natural sourced human von Willebrand Factor (vWF)
Biotin Anti-ApoA1 Binds recombinant as well as natural sourced human Apolipoprotein A1 (ApoA1)
Biotin Anti-α2-Macroglobulin Binds recombinant as well as natural sourced human α2-Macroglobulin (A2M)
Biotin Anti-Antithrombin III Binds recombinant as well as natural sourced human Antithrombin III (ATIII)
Biotin Anti-Prothrombin Binds recombinant as well as natural sourced human Prothrombin (coagulation factor II, FII)

Biotin Anti-hGH

Binds recombinant as well as natural sourced human Growth Hormone (hGH).

Biotin Anti-GCSF

Binds recombinant as well as natural sourced human Granulocyte-Colony Stimulating Factor (G-CSF)

Biotin Anti-FSH

Binds recombinant as well as natural sourced human Follicle Stimulating Hormone (FSH), through recognition of an epitope specific for intact FSH only.

Biotin Anti-Insulin
Binds recombinant and natural-sourced human insulin and shows similar binding reactivity towards porcine- and bovine-sourced insulin. It also binds to human proinsulin, the precursor of insulin that still contains the C-peptide.
Biotin Anti-EPO Binds recombinant and natural-sourced human Erythropoietin (EPO).
Biotin anti-AAV8 Binds with high affinity to Adeno-associated virus particles of serotype 8 (AAV8). Note that it may cross-bind towards other AAV serotypes.
Biotin anti-AAV9 Binds with high affinity and selectivity to Adeno-associated virus particles of serotype 9 (AAV9)
Biotin anti-FVII Binds recombinant as well as plasma derived human Factor VII (FVII)

 


More detailed information on selectivity and use of these products can be found in the product information sheets available for download on the product pages.

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