Artistic rendition of a modified strand of DNA

To support the manufacturing of Cell and Gene Therapies, we have developed innovative chromatography solutions, specifically designed to improve the downstream purification of mRNA and viral vectors such as the Adeno-Associated Virus (AAV), lentivirus, and Adv5.

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Benefit from:

  • High selectivity and capacity for the purification of viral vectors and mRNA
  • An efficient and robust purification process, from crude material in one step, offering scalability and process consistency
  • Significant improvement to the downstream process of viral vectors, by reducing the purification steps and maximizing productivity
  • An efficient and scalable solution for the purification of in vitro transcribed mRNA

AAV purification

Our proven CaptureSelect ligand technology, combined with the large pore structure of the POROS beads, enables efficient purification of large biomolecules such as viral vectors. These unique affinity resins are developed to significantly improve downstream processing of viral vectors by reducing the number of purification steps and offering scalability, thus maximizing process consistency, efficiency and productivity.

POROS CaptureSelect AAV resins are unique affinity chromatography resins for the purification of Adeno-associated virus (AAV) used in gene therapy applications. 

Key benefits:

  • High dynamic binding capacity and superior resolution address capacity and selectivity requirements for purifying viral vectors
  • Robust viral clearance capabilities of the resins help to reduce the risk of possible viral contamination derived from cell culture production

When the use of your product requires further polishing, such as removal of empty capsids, Thermo Scientific POROS Anion and Cation Exchange resins can be utilized for subsequent polishing steps. The excellent resolution of these resins allow for separation of close related product forms such as empty and full viral capsids. 

All AAV affinity purification and polishing resins come with full regulatory support, enabling use in commercial manufacturing. 

For more information and products in the AAV manufacturing workflow, please visit our page on AAV production.

Table 1. Summary of POROS CaptureSelect AAV affinity resin properties

 Binding capacity (vg/mL)*Serotype affinity
POROS CaptureSelect AAVX>1014AAV1, AAV2, AAV3, AAV4, AAV5. AAV6, AAV7, AAV8, AAV9, recombinant and chimeric vectors**
POROS CaptureSelect AAV9>1014AAV9
POROS CaptureSelect AAV8>1013AAV8

* Measured as viral genomes per milliliter (vg/mL).
** The AAVX Affinity ligand has demonstrated binding reactivity towards a large set of AAV serotypes (including chimeric vectors), suggesting that an even broader range of AAV serotypes might be purified by this resin.

Resins and reagents for the purification of AAV – ordering information

Adenovirus-5 (AdV5) purification

To support the research and development of Adenoviral vectors, a resin for the purification of Adenovirus serotype 5 (AdV5) was developed. POROS CaptureSelect AdV5 Affinity Matrix is specifically designed for the purification and/or scavenging of recombinant hexon capsomeres of AdV5 particles, as well as free hexon proteins. The AdV5 affinity matrix is a research-use-only chromatography resin but can be further developed into a bioprocess resin suitable for cGMP manufacturing.

Lentivirus purification solutions

Heparin affinity chromatography is a simple and effective way of purifying proteins containing a heparin binding domain. Due to its important biological function, the domain is often easy accessible, allowing for efficient purification. POROS 50 HE Heparin Affinity resin can be used for the purification of viral vectors containing a Heparin binding site, such as Lentivirus or Baculovirus.

Alternatively, anion exchange chromatography can serve as an ideal solution for the purification of lentiviral particles. The features of POROS D50 resin, a weak anion exchanger, make it suitable for lentivirus capture.

Resins and reagents for the purification of Lentivirus - ordering information

mRNA purification

To support messenger RNA (mRNA)-based vaccines and therapies, Thermo Scientific POROS Oligo (dT)25 affinity solution was designed for the purification and isolation of mRNA of in vitro transcription manufacturing processes. Tailor-made for efficient purification of mRNA constructs, the POROS Oligo (dT)25 affinity resin demonstrates: 

  • Simple mRNA capture through AT base-pairing
  • Ease of use – load in NaCl and elute in water
  • High dynamic binding capacity
  • High product yield and recovery
  • Excellent impurity removal
  • Scalable and suitable for commercial manufacturing
  • Reduces the complexity of subsequent polish steps
Resins and reagents for the purification of mRNA - ordering information

Video - Efficient and scalable purification of mRNA using affinity chromatography

PDF titled Supporting development of mRNA-based therapies by addressing large-scale purification challenges

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Bioprocessing resources

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For research use or further manufacturing, not for diagnostic use or direct administration in humans or animals.