We have developed a unique portfolio of affinity resins helping you to develop next generation antibody therapeutics. These affinity resins can be used for clinical and commercial production and include:
  • a platform solution for manufacturing of Fab fragments, irrespective of the type of light chain
  • affinity resins offering mild elution conditions for Fc-fusion proteins and pH sensitive IgGs
  • a cost-effective, high performance Protein A resin developed from in-house sourced Protein A

Next to the affinity resins we also offer product specific ELISAs to measure any ligand potentially leaching from the column.

The image below depicts where the various products bind the antibody or antibody fragment.


CaptureSelect affinity matrices from Thermo Fisher Scientific:

Product

Target applications

Uniqueness

RoboColumn®^

CaptureSelect IgG-CH1

Human IgG antibodies and Fab fragments thereof

CH1 domain binding, no free light chain binding for fragments

 

CaptureSelect KappaXL

Human IgG antibodies and Fab fragments thereof containing a kappa light chain

Mild elution for fragments and antibodies

X

CaptureSelect FcXL

Human IgG antibodies and Fc-fusion proteins

CH3 domain binding, human-specific, recognizing all four subclasses, mild elution conditions

X

POROS  
MabCapture A Select Resin
Monoclonal antibody capture applications Major increase in process productivity and reduction in downstream bottleneck X

^RoboColumn® is a product of Repligen Corp.

View additional CaptureSelect products ›

Ligand conjugates

HPLC affinity columns

Pre-packed columns

When your specific process needs cannot be met with current products, our custom chromatography services are available to build you a unique solution.

For more information ›

Contact a member of our business development team to discuss possibilities for further development of these products.

CaptureSelect information


CaptureSelect Leakage ELISAs

Products incorporating CaptureSelect technology are used for clinical and commercial manufacturing of a variety of biologicals. To support these products Thermo Fisher Scientific has developed product-specific Leakage ELISAs that can be used for routine quality analysis. Below is a list of Leakage ELISAs and their corresponding affinity resins.

Product Complementing resin
CaptureSelect IgG-CH1 Ligand Leakage ELISA
CaptureSelect IgG-CH1 Affinity Matrix
CaptureSelect CH1-XL Ligand Leakage ELISA CaptureSelect CH1-XL Affinity Matrix
CaptureSelect KappaXL Ligand Leakage ELISA CaptureSelect KappaXL Affinity Matrix
CaptureSelect FcXL Ligand Leakage CaptureSelect FcXL Affinity Matrix
CaptureSelect Kappa Select Leakage ELISA Kit KappaSelect *
CaptureSelect Lambda Fab Select Leakage ELISA Kit LambdaFabSelect *
CaptureSelect Ig Select Leakage ELISA Kit IgSelect *

Resources

Application notes

New article

  • Evaluation of Strategies to Control Fab Light Chain Dimer During Mammalian Expression and Purification: A Universal One-Step Process for Purification of Correctly Assembled Fab. Jennifer Spooner, Jenny Keen, Kalpana Nayyar, Neil Birkett, Nicholas Bond, David Bannister, Natalie Tigue, Daniel Higazi, Benjamin Kemp, Tristan Vaughan, Alistair Kippen, Andrew Buchanan (2015) Biotechnol Bioeng. 2015 Jul;112(7):1472-7.

For more information on CaptureSelect