"Sequencing cancer samples with Ion AmpliSeq™ gene panels and the Ion PGM™ sequencer, you can describe heterogeneity of samples according to the mutations found in the sub-populations. This is impossible to do with current methods."

Aldo Scarpa, MD, PhD
Professor of Pathology Director
Department of Pathology and Diagnostics Director
ARC-NET Research Centre for Applied Research on Cancer University of Verona
Life Technologies OncoNetwork Consortia Member

"That’s why we need to have a panel that allows us to identify all the mutations, all of the molecular alterations, that are present in the specific tumor, so we can really see the molecular landscape of each individual tumor."

Nicola Normanno, MD, PhD
Director, Research Department Centro di Ricerche Oncologiche di Mercogliano
Mercogliano (AV) and INT-Fondazione Pascale
Naples, Italy
Life Technologies OncoNetwork Consortia Member

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"Ion PGM System with Ion AmpliSeq™ technology has allowed us to substantially improve the turnaround time and cost of sequencing these important genes—BRCA1 and BRCA2."

José Luis Costa, PhD
Postdoctoral Fellow, Institute of Molecular Pathology and Immunology Porto, Portugal
Life Technologies BRCA1 and BRCA2 Consortia Member

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"Sequencing gene panels with Ion Torrent™ is complete. You have the Ion AmpliSeq™ gene selection technology, the Ion PGM™ sequencing machine and also the Ion Reporter™ analysis software. For me, it is important to have a whole integrated solution so researchers can advance from the tumor to results."

Pierre Laurent-Puig, MD, PhD
Professor, Paris-Descartes University Medical School
Paris, France
Life Technologies OncoNetwork Consortia Member

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"The methodology proved robust enough to call even difficult mutations, including homopolymers of 6-8 bases in BRCA1 and BRCA2 genes."

José Luis Costa, PhD
Postdoctoral Fellow, Institute of Molecular Pathology and Immunology
Department of Pathology and Diagnostics Director
Porto, Portugal
Life Technologies OncoNetwork Consortia Member

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"Using other technologies, for every PCR reaction we would need 10 ng of DNA, and now with Ion AmpliSeq™ Colon and Lung Cancer panel, we can interrogate tens of genes in one reaction—just 10 ng."

Marjolijn Ligtenberg, PhD
Associate Professor, Head of Laboratory Tumor Genetics,
Radboud University Nijmegen Medical Centre
Nijmegen, the Netherlands
Life Technologies OncoNetwork Consortia Member

"You can get results on 20, 30, 40 genes by using 10 ng of DNA from formalin fixed paraffin embedded
tissue."

Nicola Normanno, MD, PhD
Director, Research Department
Centro di Ricerche Oncologiche di Mercogliano
Mercogliano (AV) and INT-Fondazione Pascale
Naples, Italy
Life Technologies OncoNetwork Consortia Member

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For Research Use Only. Not for use in diagnostic procedures.