Technical and educational videos for cell analysis topics

Cell Analysis Educational Videos & Webinars

These videos, tutorials and webinars are designed to provide technical and education information for cell analysis platforms, applications and techniques.

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How the Click-iT Plus EdU Proliferation Assay works

Measuring DNA synthesis is the most precise way to detect changes in cell proliferation. Image-based proliferation assays generate spatial and temporal results that cannot be detected with other methods. This video describes and compares two of the most referenced image-based proliferation technologies.

Webinar—Breathing new life into hypoxia research

Although the significance of hypoxia in biological processes is well known, creating model systems with accurate control of hypoxic conditions is extremely difficult without access to elaborate systems that allow precise control and maintenance of temperature, humidity, and gases (CO2 and O2) during an experiment. In this webinar we cover:

  • Overview of hypoxia in human diseases
  • Classical methods for setting up hypoxic conditions
  • Novel instruments and reagents for imaging cells in hypoxic conditions

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Tutorial 1.1 Culture conditions–Fixed cell imaging: 5 steps for publication-quality images  
The first step in obtaining a good image is tissue preparation. For cultured cells, the cells must have good cell health and morphology, as well as good confluency. Healthy cells equal healthy data.
EVOS, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, imaging hardware, imaging systems, immunofluorescence (IF), microscope
Tutorial 1.2 Fixation–Fixed cell imaging: 5 steps for publication-quality images  
The next step of tissue preparation is fixation. Fixation refers to a chemical means of killing and preserving cells in a particular physiological state, and in many cases, to preserve morphology. Proper fixation equals preservation of target.
fixation, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF)
Tutorial 1.3 Permeabilization–Fixed cell imaging: 5 steps for publication-quality images  
The next step is the permeabilization of the cells which is the key to opening intracellular compartments.
cell permeabilization, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF)
Tutorial 1.4 Blocking–Fixed cell imaging: 5 steps for publication-quality images  
The next step after permeabilization is blocking, and there are a number of blocking techniques. Protein blocking equals specific antibody binding. Dye charge blocking means less non-specific binding.
dye charge blocking, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), nonspecific binding, protein blocking
Tutorial 1.5 Autofluorescence–Fixed cell imaging: 5 steps for publication-quality images 
The last step in cell preparation is autofluorescence. Cells and tissue can have a certain degree of autofluorescence that can confuse the specific signal, and lower the signal-to-background. Overcoming autofluorescence means greater sensitivity.
autofluorescence, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), tissue autofluorescence
Tutorial 2.1 Primary antibody choice–Fixed cell imaging: 5 steps for publication-quality images 
After preparation, the second step to publishable images is to label the sample, usually involving primary antibodies to your specific targets of interest. The antibody source, the use of direct versus labeled antibodies as well as the validation for specific applications is discussed.
antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), primary antibodies
Tutorial 2.2 Primary antibody protocol optimization–Fixed cell imaging: 5 steps for publication-quality images 
Every primary antibody must be optimized separately. There are many protocols available, and it is important to understand a "one size fits all" approach gives inferior results, as every antibody is slightly different. Learn how to approach optimization.
antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), primary antibodies, primary antibody protocol
Tutorial 3.1 Secondary antibody choice–Fixed cell imaging: 5 steps for publication-quality images 
Step three of the five steps in making publishable images is to detect the label. That is, to detect with a secondary antibody, for instance, or an amplification technique, as well as to determine what controls to use. Your options are discussed.
antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), secondary antibodies, secondary antibody, secondary detection
Tutorial 3.2 Secondary antibody optimization–Fixed cell imaging: 5 steps for publication-quality images 
Secondary antibody detection protocols also need to be optimized for each primary antibody used.
antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), secondary antibodies, secondary antibody protocol, secondary detection
Tutorial 3.3 Amplification techniques–Fixed cell imaging: 5 steps for publication-quality images 
If the signal is not strong enough using standard secondary detection schemes, you can increase the signal using amplification techniques. This is particularly important for low-expressing antigens, or rare-cell detection in samples. Examples are discussed.
biotin and streptavidin, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), signal amplification, tyramide detection
Tutorial 3.4 Controls–Fixed cell imaging: 5 steps for publication-quality images 
Researchers should conduct all necessary controls to rule out the possibility of non-specific binding or non-specific signal. Types of controls are described. Proper controls will boost your confidence in your final results.
fixed-cell imaging, fluorescence microscopy/fluorescence imaging, imaging controls, immunofluorescence (IF), sample controls
Tutorial 3.5 Dye choice and special concerns–Fixed cell imaging: 5 steps for publication-quality images 
There are many different dyes spanning the visible, far-red, and infrared wavelengths.Considerations for making the right choices for your experiment are presented.
dye choice, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, Fluorescence SpectraViewer, immunofluorescence (IF), label choice, muliparametric dye selection
Tutorial 4.1 Mounting media–Fixed cell imaging: 5 steps for publication-quality images 
Using the right mounting media can impact your experiment. Be sure to choose the right type of mountant for your set-up.
antifade mounting media, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), mounting media
Tutorial 4.2 Photobleaching and antifades–Fixed cell imaging: 5 steps for publication-quality images 
What is photobleaching and how can you prevent it from destroying your sample? Options for antifades are discussed.
antifades, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), photobleaching, Prolong Gold, Vectashield
Tutorial 5.1 Imaging platforms-hardware–Fixed cell imaging: 5 steps for publication-quality images 
The fifth step of the process is the actual imaging. To capture top-quality images, you need an imaging platform with top-of-the-line imaging capabilities. Here we review considerations for getting the best image.
EVOS, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, imaging hardware, imaging systems, immunofluorescence (IF), microscope
Tutorial 5.2 Imaging platforms-software–Fixed cell imaging: 5 steps for publication-quality images 
Taking images on a microscope usually entails having some type of imaging software that aids in taking the image and assists in combining differing colors into one. There are some very important aspects to keep in mind to get a publishable image.
FiFI software, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, ImageJ, imaging software, imaging systems, immunofluorescence (IF)
Tutorial 5.3 Image capture with EVOS FL Auto 2.0–Fixed cell imaging: 5 steps for publication-quality images 
Here the advantages of using the EVOS FL Auto 2.0 imaging system to capture your images are discussed.
EVOS FL Auto 2.0, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, image capture, immunofluorescence (IF)
Tutorial 5.4 Image analysis with Celleste software–Fixed cell imaging: 5 steps for publication-quality images 
The functionality of the Celleste softwis reviewed and considerations for processing the image in different software programs are described.
Celleste, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, image analysis, immunofluorescence (IF)
Tutorial 5.5 Ethical considerations–Fixed cell imaging: 5 steps for publication-quality images 
Ethical imaging means trustworthy data, and thus, publishable data. How to treat your samples and data to preserve data integrity is presented.
ethical considerations, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, image analysis, immunofluorescence (IF), sample manipulation
Tutorial HCA Q & A 
A video series that answers common questions about high content analysis and the different Thermo Scientific high content imaging platforms
ArrayScan, CellInsight, high content analysis
Tutorial Labeling a purified antibody  
Educational video on how to label your next antibody for imaging or fluorescence. Walk through the tutorial as Molecular Probes scientists demonstrate the protocol—including all the tips and tricks you'll want to know about for your next antibody labeling experiment. The video features Judie showing Curtis, a chemistry graduate student, how to label his monoclonal antibodies while saving time and maximizing yield.
antibodies, antibody labeling, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF)
Tutorial Molecular Probes tutorial series—Analyzing flow cytometry data  flow cytometer calibration, flow cytometer/low cytometry, multicolor flow cytometry
Tutorial Molecular Probes tutorial series—Anatomy of fluorescence spectra  absorbance, emission, excitation, spectra
Tutorial Molecular Probes tutorial series—Introduction to flow cytometry  flow cytometer calibration, flow cytometer/flow cytometry, multicolor flow cytometry
Tutorial Molecular Probes tutorial series—Introduction to fluorescence  fluorescence
Tutorial Molecular Probes tutorial series—Overview of filters and light sources  emission, excitation, filters, light cubes, light sources
Video Adding oil to objectives on the EVOS FL Auto 2 Microscope 
See how easy it is to add oil to your objective without having to remove your sample when imaging with the EVOS FL Auto 2 Microscope.
EVOS FL Auto 2 Microscope, fluorescence microscopy/fluorescence imaging, instrument set-up
Video Batch processing: Celleste Image Analysis Software brightfield microscopy, Celleste, fluorescence microscopy/fluorescence imaging, imaging analysis
Video Beginner's guide to macro writing: Celleste Image Analysis Software
brightfield microscopy, Celleste, fluorescence microscopy/fluorescence imaging, imaging analysis
Video Cell and object counting: Celleste Image Analysis Software brightfield microscopy, Celleste, fluorescence microscopy/fluorescence imaging, imaging analysis
Video CellEvent Caspase 3/7 reagent and apoptosis detection 
HeLa cells were loaded with 50 nM TMRM (red) followed by 5 μM CellEvent Caspase 3/7 Substrate (green). Cells were then treated with 0.5 μM staurosporine to induce apoptosis. TMRM detects the mitochondrial membrane potential and the signal disappears as the apoptosis onsets. CellEvent detects apoptosis, with green signal.
apoptosis, cell health, CellEvent, fluorescence microscopy/fluorescence imaging, live-cell imaging
Video CellTracker Violet reagent and mitosis 
U-2 OS cells were transduced with CellLight Tubulin-GFP and Cellular Lights Actin-RFP. The following day cells were labeled with 5uM CellTracker Violet BMQC for 30 minutes at 37C in complete media and washed in fresh media. Images were taken every 5 minutes for 16 hours.
cell structure-all, cell tracking, fluorescence microscopy/fluorescence imaging, live-cell imaging
Video Click-iT Plus EdU Proliferation Assay 
Measuring DNA synthesis is the most precise way to detect changes in cell proliferation. Image-based proliferation assays generate spatial and temporal results that can not be detected with other methods. This video describes and compares two of the most referenced image-based proliferation technologies.
cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging
Video Click-iT Plus TUNEL apoptosis assays 
The Click-iT Plus TUNEL assay detects apoptotic cells in tissue and cultured cell samples through the use of a small, highly specific labeling moiety and a bright fluorescent dye. After incorporation of the labeling moiety into DNA fragments, detection is achieved through a catalyzed "click" reaction using conditions mild enough to preserve the fluorescent signal from GFP or RFP.
apoptosis, Click-iT, fluorescence microscopy/fluorescence imaging
Video Compilation of live cell imaging videos using Invitrogen fluorescent reagents 
This video demonstrates novel product brands from Invitrogen for live cell imaging include CellLight targeted fluorescent proteins, CellROX reagents for oxidative stress, CellEvent caspase 3/7 detection reagent, and many more fluorescent dyes and probes.
cell structure-mitochondria, CellEvent, CellLight, CellROX, fluorescence microscopy/fluorescence imaging, live-cell imaging, oxidative stress
Video Create z-stacks using the EVOS FL Auto 2 Microscope 
Capturing images at different Z-planes can be a powerful tool in fluorescence microscopy. This capability can reveal conditions not seen with standard widefield imaging and easier than ever to use with the EVOS FL Auto 2 microscope.
EVOS FL Auto 2 Microscope, fluorescence microscopy/fluorescence imaging, instrument set-up
Video Detection of oxidative stress with CellROX Green in U2-OS cells 
U2-OS cells were plated at 200,000 cells per dish on a 35 mm glass bottom dish (MatTek) and cultured overnight. The cells were rinsed once and loaded with 5 µM CellROX Green Reagent (Cat. No. C10444) and 100 nM TMRM in Live Cell Imaging Solution (LCIS, Cat. No. A14291DJ) for 15 minutes at 37 degrees C. After loading, cells were imaged live with no washing every 20 seconds for seventy minutes on the DeltaVision Core inverted microscope at 37 degrees C. Menadione was added to a final concentration of 100 uM after ten minutes of baseline acquisition. The time lapse data shows the loss of signal from TMRM in the red channel as mitochondrial function decreases, concomitant with the onset of a nuclear signal in the green channel as CellRox Green is oxidized and the reagent migrates to the nucleus to generate a fluorogenic response as the active form of the dye binds to DNA.
CellROX, fluorescence microscopy/fluorescence imaging, live-cell imaging, oxidative stress
Video Exosomes—The Next Small Thing: Episode 1—What is an exosome? Exosomes
Video Exosomes—The Next Small Thing: Episode 2—The history and promise of an exosome Exosomes
Video Exosomes—The Next Small Thing: Episode 3—Exosomes in cancer research Exosomes
Video Exosomes—The Next Small Thing: Episode 4—Curiosity and a passion for science Exosomes
Video Exosomes—The Next Small Thing: Episode 5—Collaboration—The key to scientific success Exosomes
Video Exosomes—The Next Small Thing: Episode 6—Exosomes the next small thing Exosomes
Video How to add a scale bar and grid to cell images on the EVOS FL Auto 2 Microscope 
See how easy it is to add a scale bar and grid to your live or captured cell images on the EVOS FL Auto Microscope. You can adjust the grid size, color, and alignment to fit the needs of your experiment.
EVOS FL Auto 2 Microscope, fluorescence microscopy/fluorescence imaging, instrument set-up
Video How to setup vessel maps on the EVOS FL Auto 2 Microscope 
The EVOS FL Auto 2 can accommodate most vessel types and sizes, including slides, multi-well plates, culture flasks, and petri dishes. See how easy it is to setup the EVOS FL Auto 2 for imaging with the vessel maps tool.
EVOS FL Auto 2 Microscope, fluorescence microscopy/fluorescence imaging, instrument set-up
Video How to site specifically label your antibody using SiteClick technology 
This video explains how to easily and site specifically label an antibody using an enzymatic and Click chemistry approach. This method can be applied to any intact IgG antibody and requires no antibody engineering or complex methodology. Unlike classic antibody conjugation techniques this breakthrough SitClick technology allows for antibody conjugation with complete confidence that the label will not directly interfere with the antibody binding domain.
antibodies, antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, Qdot, western detection
Video Immunohistochemistry—Tips and tricks for high background Immunohistochemistry application offers a spatial visualization and location of an antigen in tissue sections. This video offers troubleshooting tips for issues falling into the "high background" category for IHC.
antibodies, brightfield microscopy, colorimetric, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC), secondary detection
Video Immunohistochemistry—Tips and tricks for weak or no stain Immunohistochemistry application, offers a spatial visualization and location of an antigen in tissue sections. 
antibodies, brightfield microscopy, colorimetric, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC), secondary detection
Video LIVE DEAD Cell Imaging kit on the EVOS Auto Imaging System 
Time course of cell death visualized using the LIVE/DEAD Cell Imaging kit (R37601). The LIVE/DEAD Cell Imaging kit is based on a cell-permeable dye (calcein, AM) that stains live, viable cells bright green and a cell-impermeable red marker that only stains dead and dying cells, which are characterized by compromised cell membranes. Labeled U-2 OS cells were treated 1 μM staurosporine and fluorescence images in the FITC and TexasRed channels were acquired every 5 minutes over 14 h on the EVOS Auto Imaging System using a 20x objective.
cell viability, fluorescence microscopy/fluorescence imaging, ion channels, ion flux, ion indicators, live-cell imaging
Video Mitochondrial dynamics through cell division 
U2OS cells were transduced with CellLight Mito-RFP and imaged every 5 minutes for 16 hours. Extensive mitochondrial motility is seen throughout mitosis and as the cell regains it's pre mitotic shape following mitosis.
cell structure-mitochondria, CellLight, fluorescence microscopy/fluorescence imaging, live-cell imaging
Video Mounting a Coverslip 
Molecular Probes School of Fluorescence explains step-by-step how to mount a coverslip properly for cellular imaging. Formulations of mounting media that can add favorable properties such as optimizing the refractive index to match that of glass, preventing photobleaching, or preserving samples for long-term storage are widely available.
fluorescence microscopy/fluorescence imaging, imaging sample preparation
Video Regions of interest: Celleste Image Analysis Software
brightfield microscopy, Celleste, fluorescence microscopy/fluorescence imaging, imaging analysis
Video Reviewing Cell Images on the EVOS FL Auto 2 Microscope 
Cell images can be saved from the EVOS FL Auto 2 as a single field, scan, time lapse, and z-stack. Easily review your images and metadata or make adjustments to image brightness, contrast, or gamma and resave your images with the new settings.
EVOS FL Auto 2 Microscope, fluorescence microscopy/fluorescence imaging, instrument set-up
Video Setting up an automated scan routine on the EVOS FL Auto 2 Microscope 
Save time when imaging cells by easily setting up an automated scan routine using the EVOS FL Auto 2 microscope.
EVOS, fluorescence microscopy/fluorescence imaging, instrument set-up
Video The secret to solving the most common problem in IF experiments
cell analysis antibodies, cell imaging, fluorescence microscopy/fluorescence imaging, fixed-cell imaging, immunofluorescence (IF)
Video The two worlds of cell separation 
cell expansion, cell isolation, Dynabeads, magnetic beads
Video Time-lapse of cell migration and apoptosis during angiogenesis 
Angiogenesis pseudo-tube formation and apoptosis shown with time-lapse imaging. The time-lapse images were captured and assembled using an EVOS FL Auto Imaging System equipped with the EVOS Onstage Incubator.
angiogenesis, apoptosis, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, live-cell imaging, onstage incubator
Video Time-lapse with Qtracker 655 cell labeling reagent 
Time-lapse image of live cells labeled with Qtracker reagents. Adherent HeLa cells were incubated with Qtracker VIVID 655 Cell Labeling Kit (Cat. No.Q25021MP) at 10 nM for one hour. Images were collected over 90 min using a Zeiss LSM 710 confocal microscope and are shown pseudo-colored magenta.
fluorescence microscopy/fluorescence imaging, live-cell imaging
Video Tips and tricks: Phospho flow cytometry 
Tips and tricks to help you successfully study phosphorylated proteins using flow cytometry.
antibodies, cell signaling, flow cytometer/flow cytometry, intracellular flow cytometry, phosphorylation
Video Tips and tricks: Phospho Immunofluorescence 
 
cell analysis antibodies, cell imaging, fluorescence microscopy/fluorescence imaging, fixed-cell imaging, immunofluorescence (IF)
Video Using the locations tool on the EVOS FL Auto 2 Microscope 
Learn how to create custom locations to use in automated routines for scanning and time lapse experiments on the EVOS FL Auto 2 Cell Imaging System.
EVOS, fluorescence microscopy/fluorescence imaging, instrument set-up
Video Viability determination of HeLa cells using ReadyProbes Cell Viability Imaging Kit (Blue/Green) 
HeLa cells were loaded with NucBlue Live and NucGreen Dead (using 2 drops per ml) in complete media for 15 minutes at 37C. Stauroporine was then added to a final concentration of 1 µM and images were acquired every 30 min. for 18 hours using EVOS Auto Imaging System. All cells are stained with NucBlue Live, shown with blue nuclei. Over time an increase in the number of dead cells is observed as indicated by the appearance of green nuclei (NucGreen Dead).
cell structure-nucleus, cell viability, fluorescence microscopy/fluorescence imaging, live-cell imaging
Video Washing your sample 
Molecular Probes School of Fluorescence explains the wash step common to many cellular imaging protocols.
fluorescence microscopy/fluorescence imaging, imaging sample preparation
Video Wound healing and cadherin-4 expression  
Murine glioma cells were transfected with a DsRed-cadherin-4 fusion, which plays a critical role in cell migration during wound repair, or a GFP control plasmid, then mixed with wild-type cells and seeded in a 24-well plate. After scratch wounding, cell migration was followed over a 21 h period on the EVOS Cell Imaging System equipped with an EVOS Onstage Incubator using a 10X phase objective. Images were captured at an interval of about 10 minutes (116 images total). Video courtesy of Paolo Malatesta, PhD., IRCCS Azienda Ospedaliera Universitaria San Martino — IST, Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
EVOS, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging, wound healing
Video Z Stack image of HeLa cells labeled with CellLights reagents  
A series of images were captured on the EVOS FL Auto Cell Imaging System. Creating a Z-stack from these images allowed the observation of cellular cytoskeletal changes, which can be indicative of the loss of cell health. Methods HeLa cells grown in MatTek 6-well glass bottom culture plates were transduced with CellLights Tubulin-GFP and CellLight Mitochondria-RFP overnight at 37oC. The following day, NucBlue Live reagent (2 drops/mL) was added to the cultures. Cells were then imaged on an EVOS FL Auto Cell Imaging System with 100x oil immersion objective using the Z-stack function. The step size was set using the Nyquist formula and performed at 0.366 μm.
cell health, cell structure-all, CellLight, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging
Webinar A comparison of basic immunofluorescent labeling strategies  
In this free webinar, we will compare different immunofluorescent labeling strategies exploring the pros and cons of each method. You will learn when the use of a direct conjugate is appropriate and when amplification techniques can be utilized. We'll also present a simple decision tree to aid in determining the best method for each situation.
Alexa Fluor, antibodies, antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunocytochemistry (ICC), immunofluorescence (IF)
Webinar A practical approach to antibody labeling  
The growing number of fluorophores available makes labeling your own antibodies a tempting proposition. But with many antibody labeling solutions available, selecting the best option can be a daunting task. In this webinar we will: Provide an overview of our antibody labeling kits Offer guidance on which methods are ideal for specific applications and experiments Provide tips and tricks to optimize your labeling protocol
antibodies, antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF)
Webinar An introduction to flow cytometric analysis, Part 1: Cell proliferation analysis  
In this 2-part series, we will give an overview of tools and techniques using Invitrogen reagents for flow cytometric analysis of cell proliferation, viability, vitality, and apoptosis.
apoptosis, cell cycle, cell proliferation, flow cytometer/flow cytometry, multicolor flow cytometry, viability
Webinar An introduction to flow cytometric analysis, Part 2: Cell viability and apoptosis analysis  
In this free webinar, we will discuss flow cytometric analysis of apoptosis and identification of dead cells. Changes introduced by apoptosis can be tested with numerous assays measuring Membrane structure, Mitochondrial function, Metabolism, Caspase activity, Membrane integrity, and DNA fragmentation. We will also discuss dead cell identification using traditional impermeant nucleic acid dyes.
apoptosis, cell cycle, cell proliferation, flow cytometer/flow cytometry, multicolor flow cytometry, viability
Webinar An introduction to immunofluorescence staining of cultured cells  
In this webinar, we discuss the steps of an immunofluorescent staining protocol including material list, common variations, and necessary controls. We'll also provide a simple troubleshooting guide and examine how to avoid common pitfalls. Presented by Jason Kilgore, Technical Support Specialist, Thermo Fisher Scientific.
antibodies, antibody labeling,antifades, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF)
Webinar Basic techniques in autophagy research  This webinar will introduce you to a series of analytical tools and techniques to help you identify and interrogate key features of autophagy. Topics to be covered include: Tips and tricks for selecting the right tools and achieving the best results Fluorescent proteins and antibodies used to analyze both live and fixed cells Analysis steps using a variety of multiplexing options, with quantitative methods for image analysis or fluorescence intensity measurement
autophagy, cell health, fluorescence microscopy/fluorescence imaging, fluorescent proteins, high content analysis, immunofluorescence (IF)
Webinar Basics of flow cytometry, Part I: Gating and data analysis  
An introduction to fluorescence, this presentation covers the following topics: the principle of the flow cytometry platform, the basic components of a flow cytometer, how to interpret a dye excitation/emission spectrum, how data is displayed, basic gating demonstration, and common statistics and terminology used in flow cytometry.
flow cytometer calibration, flow cytometer/flow cytometry, flow cytometry data, flow cytometry set up, multicolor flow cytometry
Webinar Basics of flow cytometry, Part II: Compensation  
This presentation provides an overview of basic fluorochromes used in flow cytometry. Topic includes: the principle of compensation, how to perform compensation, the types of controls recommended and their use, basic strategies for designing a flow experiment, and data presentation.
flow cytometer calibration, flow cytometer/flow cytometry, flow cytometry data, flow cytometry set up, multicolor flow cytometry
Webinar Basics of multicolorflow cytometry panel design  
With the proliferation of new fluorescent dyes, as well as instruments that can detect 18 or more parameters multicolor flow cytometry has become more popular and more accessible than ever. This webinar presented by Dr. Holden T. Maecker at Stanford University will discuss the caveats of good panel design, including: Rules for designing panels Examples and practical application of these rules Controls and standardization Relevance of panel design to new mass cytometry platforms
antibodies, compensation, flow cytometer/flow cytometry, immunophenotyping, multicolor flow cytometry, viability
Webinar Breathing new life into hypoxia research  
Although the significance of hypoxia in biological processes is well known, creating model systems with accurate control of hypoxic conditions is extremely difficult without access to elaborate systems that allow precise control and maintenance of temperature, humidity, and gases (CO2 and O2) during an experiment. In this webinar, we will discuss: Overview of hypoxia in human diseases Classical methods for setting up hypoxic conditions Novel instruments and reagents for imaging cells in hypoxic conditions
EVOS, fluorescence microscopy/fluorescence imaging, high content analysis, hypoxia, onstage incubator
Webinar DNA content cell cycle analysis using flow cytometry 
Find out how careful acquisition and methodical preparation contribute to accurate and consistent DNA analysis. In this webinar we'll discuss: An overview of the methods and materials for using flow cytometry to determine cell cycle by measuring DNA content Selection of DNA dyes for live cell and fixed cell analysis Tips and tricks for consistent results
cell counting, cell cycle, cell proliferation, flow cytometer/flow cytometry
Webinar Fixed cell imaging—Five steps for publication-quality images 
With over 40 years dedicated to cell imaging research, we offer long-proven tools and protocols to help confidently create quality cell images the first time. This on demand webinar covers the 5 essential steps to getting great images.
antibodies, blocking, Celleste, dyes, EVOS FL Auto 2.0, fixation, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), sample detection, sample labeling, sample preparation, signal amplification
Webinar Flow cytometry in microbiological research  
In recent years the application of flow cytometry in microbiological research has expanded from detection and quantification of organisms to more complex studies including analysis of host-microbe interactions and detailed spatial and temporal analysis of microbial metabolism in different environments. During this webinar we will discuss how the multi-parametric nature of flow cytometry can be applied to microbiology and the advantages of using this application over traditional microbiological methods.
flow cytometer/flow cytometry, microbiology, multicolor flow cytometry, viability
Webinar From the hood to the microscope; Revolutionizing cell-based imaging 
Join us for an interactive, educational webinar that showcases some of the latest advancements in cell-based research and imaging. Learn as we explore how to obtain superior results through the careful selection of reagents and the optimization of your imaging workflow— from growing cells on suitable surfaces through to image capture. Particular attention will be given to growing and monitoring cells for imaging, fluorescent labeling of live cells, critical considerations for time-lapse imaging and optimizing live cell imaging. Topics include: Choose a suitable imaging culture vessel for studying cell growth and viability using bright-field microscopy Label cells to maximize signal-to-noise for fluorescence imaging Prepare culture conditions and capture images without losing temporal data for time-lapse imaging Achieve hypoxic culture conditions by modifying gas conditions or employing cell spheroid cultures
cell health, EVOS, FLoid, fluorescence microscopy/fluorescence imaging, hypoxia, live-cell imaging, onstage incubator
Webinar Introduction to basic cytoskeleton labeling and detection  
The cytoskeleton is a key component of mammalian cells, providing the framework for cell migration and intracellular transport, furthermore the cytoskeleton regulates cell size and shape as well as important processes such as mitosis and endocytosis. We offer a number of solutions for researchers using fluorescent probes to study the cytoskeleton. This webinar will provide an overview of the structures that comprise the cytoskeleton and important experimental parameters. The webinar also offers a comprehensive guide to available labeling and detection technologies for cytoskeletal research as well as tips and tricks on how to best use them. These tools include those for live-cell imaging fluorescent dyes, antibodies and the BacMam gene delivery platform.
cell structure-actin, cytoskeleton, endocytosis, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins
Webinar Learn to choose the right fluorophore when designing experiments 
The choice of fluorophore is one of the first important decisions to make in developing an experiment. Fluorophores are compounds that emit light at a specific wavelength when they have been excited at another, lower wavelength. Join our webinar and explore: How to choose the best organic dye for an assay Quantum dots and how they compare to other dyes When to use a phycobiliprotein like R-PE or APC When to use fluorescent proteins like GFP How to choose a suitable dye to match your instrument In addition, we will explore the basic characteristics, strengths, and weaknesses of the various fluorophores to help you choose and develop the best assay for your needs.
antibodies, antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins
Webinar The meaning of life at the cellular level: detecting apoptosis with fluorescence  
In this webinar, Thermo Fisher Scientific scientists will demonstrate several solutions using fluorescent probes to study key components of the apoptotic machinery. The presentation includes: An overview of the features of apoptosis Key parameters that can be measured to assay apoptosis A comprehensive guide to available labeling and detection technologies for apoptosis research Tips and tricks to best implement those technologies
apoptosis, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent proteins
Webinar The meaning of life at the cellular level: Probing viability with fluorescence  
This webinar will provide an overview of features of healthy and unhealthy cells as well as describing key parameters that can be measured to assay cell viability. The webinar also offers a comprehensive guide to available labeling and detection technologies for cell health research as well as tips and tricks on how to best use them.
cell health, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, viability
Webinar The meaning of life at the cellular level: Visualizing membrane trafficking  
Membrane trafficking underlies the acquisition and release of cellular material as well as the transport of macromolecules from the site of cellular synthesis to their functional location. Thermo Fisher Scientific offers a number of solutions for researchers using fluorescent probes to study key components of the endocytotic and secretory pathway. This webinar will: Provide an overview of the processes involved in membrane trafficking, along with strategies to investigate them using fluorescence microscopy Offer a comprehensive guide to available labeling and detection technologies for membrane trafficking research Provide tips and tricks on how to best implement those technologies
endocytosis, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging, membrane trafficking, particles