Cell Imaging Information

Learn about imaging microscopy and high-content analysis, including articles on new applications and techniques, webinars and videos covering everything from controls, experiment design, and more. The content covers live- and fixed-cell imaging, high-content analysis for quantitative imaging, automated imaging platforms as well as transmitted light and colorimetric imaging applications.

Cell imaging features

Guided learning

Guided learning

Molecular Probes School of Fluorescence—Imaging Basics

The Molecular Probes School of Fluorescence module on Imaging Basics is where you will learn about the fundamentals of fluorescence microscopy, find protocols for cell staining, and get troubleshooting help for your experiments.

Protocols

Protocols

Protocols for fluorescence microscopy

Find simple and easy-to-use protocols for imaging reagents and assays.


Cell imaging subtopics


Cell imaging learning resources

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TypeTitleCategories
Application note (2012)Monitoring neurite morphology and synapse formation in primary neurons for neurotoxicity assessments and drug screeningArrayScan, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, neuroscience, viability
Application note (2013)Optimizing the high content analysis of cell morphology using multi-dimensional imaging on the Thermo Scientific ArrayScan XTI HCA Reader: Stem cell coloniesAlexa Fluor, antibodies, ArrayScan, confocal microscopy, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, immunofluorescence (IF), microplate reader, particles, stem cell research
Application note (2013)Multiplexed mitosis and apoptosis analysisantibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, particles
Application note (2014)Using a high content analysis database as a repository for datadatabase management, drug discovery, high content analysis, microplate reader
Application note (2014)High content cell cycle screening: Pairing FUCCI technology with Thermo Scientific HCS Studio 2.0 Software and FCS Express Image cytometry ArrayScan, cancer, cell cycle, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader
Application note (2014)Oxidative stress measurements made easy: CellROX reagents with simple workflow for high content imaging ArrayScan, CellInsight, CellROX, high content analysis, oxidative stress
Application note (2014)Elucidating the mechanism of cell death through the use of high content analysis apoptosis, autophagy, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis
Application note (2015)Using time-lapse imaging with the EVOS FL Auto Imaging SystemEVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, hypoxia, live-cell imaging, onstage incubator
Application note (2015)Guide to setting up hypoxic conditions on the EVOS FL Auto Imaging System with Onstage IncubatorEVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, live-cell imaging, onstage incubator, phagocytosis
Application note (2015)Quantitation of proliferating cells with the EVOS FL Auto Imaging Systemantibodies, antibody labeling, ArrayScan, cell proliferation, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, onstage incubator, phagocytosis
Application note (2015)Phagocytosis visualized on the EVOS FL Auto Imaging System with Onstage IncubatorCellLight, EVOS, fluorescence microscopy/fluorescence imaging, live-cell imaging, ReadyProbes
Application note (2015)Image tiling and stitching using the EVOS FL Auto Imaging Systemcell proliferation, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, Imaging sample preparation, ReadyProbes
Application note (2015)Collecting Z-stack image sequences with the EVOS FL Auto Imaging Systemcell structure-all, CellLight, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, live-cell imaging, ReadyProbes
Application note (2015)Cell division and migration during wound healing visualized on the EVOS FL Auto Imaging Systemcell health, cell proliferation, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging
Application note (2016)Blood cell counting using the Countess II FL Automated Cell Counterautomated cell counter, brightfield microscopy, cell counting, cell counting sample preparation, Countess, EVOS, fluorescent dyes, immunophenotyping, ReadyProbes
Application note (2017)Simple and accurate monitoring of adipogenesisadipocyte, adipogenesis, automated cell counter, cell differentiation, Countess, EVOS, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, onstage incubator, stem cell research
Application note (2017)Flow cytometry analysis of transcription factor expression during differentiation of hPSC-derived cardiomyocytesantibodies, Attune/Attune NxT, cardiomyocytes, cell differentiation, flow cytometer/flow cytometry, fluorescence, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunophenotyping, stem cell research
Application note (2018)Monitoring drug-induced calcium flux in iPSC-derived cardiomyocytes 
BioProbes articles (Issues 50–present day)BioProbes Journal of Cell Biology Applicationcell analysis, flow cytometry, imaging microscopy, immunoassays, antibodies, protein detection and quantification
BookLive cell imaging Published by Wiley, this Essential Knowledge Briefing provides a short, practical guide of the background and basics of live cell imaging. Content includes basic history of live cell imaging and case studies of live cell imaging applications Common problems and solutions with live cell imaging. fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, onstage incubator
eBookLive cell imaging Published by Wiley, this Essential Knowledge Briefing provides a short, practical guide of the background and basics of live cell imaging. Content includes basic history of live cell imaging and case studies of live cell imaging applications Common problems and solutions with live cell imaging. fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, onstage incubator
Molecular Probes HandbookTSA and other peroxidase-based signal amplification techniques—Section 6.2Alexa Fluor, antibodies, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), reagents, secondary detection, signal amplification
Molecular Probes HandbookMicrospheres—Section 6.5flow cytometer calibration, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, microscope calibration, particles
Molecular Probes HandbookIntroduction to signal amplification—Section 6.1antibodies, fluorescence microscopy/fluorescence imaging, secondary detection, signal amplification, western detection
Molecular Probes HandbookFluorescence microscopy accessories and reference standards—Section 23.1fluorescence microscopy/fluorescence imaging
Molecular Probes HandbookUsing the Fluorescence Spectraviewer—Note 23.1flow cytometer/flow cytometry, fluorescence, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins
Molecular Probes HandbookSelecting optical filters for fluorescence microscopy—Note 23.2fluorescence microscopy/fluorescence imaging
Molecular Probes HandbookMitochondria in diseases—Note 12.2antibodies, cell structure-mitochondria, fluorescence microscopy/fluorescence imaging
Molecular Probes HandbookFluorescent probes for two-photon microscopy—Note 1.5fluorescence microscopy/fluorescence imaging, two-photon microscopy
Molecular Probes HandbookFluorescent probes for photoconversion of diaminobenzidine reagents—Note 14.2DAB, electron microscopy
Molecular Probes HandbookFluorescence resonance energy transfer (FRET)—note 1.2fluorescence microscopy/fluorescence imaging
Molecular Probes HandbookFluorescence polarization (FP)—note 1.4fluorescence microscopy/fluorescence imaging
Molecular Probes HandbookFluorescence correlation spectroscopy (FCS)—Note 1.3fluorescence microscopy/fluorescence imaging
Molecular Probes HandbookProbes for the endoplasmic reticulum and golgi apparatus—Section 12.4antibodies, cell structure-er, cell structure-golgi, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Molecular Probes HandbookProbes for mitochondria—Section 12.2antibodies, cell structure-mitochondria, CellLight, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Molecular Probes HandbookProbes for lysosomes, peroxisomes and yeast vacuoles—Section 12.3antibodies, cell structure-lysosomes, CellLight, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Molecular Probes HandbookProbes for the nucleus—Section 12.5antibodies, cell structure-nucleus, CellLight, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, membrane permeability
Molecular Probes HandbookA diverse selection of organelle probes—Section 12.1cell structure-er, cell structure-golgi, cell structure-lysosomes, cell structure-mitochondria, cell structure-nucleus, cell structure-plasma membrane, fluorescence microscopy/fluorescence imaging
Molecular Probes HandbookSlow-response probes—Section 22.3fluorescence microscopy/fluorescence imaging, live-cell imaging, membrane potential dyes
Molecular Probes HandbookIntroduction to potentiometric probes—Section 22.1cell structure-plasma membrane, fast-response probes, fluorescence microscopy/fluorescence imaging, fluorometer, ion channels, live-cell imaging, membrane potential, slow-response probes
Molecular Probes HandbookFast-response probes—Section 22.2cell structure-plasma membrane, fast-response probes, fluorescence microscopy/fluorescence imaging, fluorometer, ion channels, live-cell imaging, membrane potential
Molecular Probes HandbookOther nonpolar and amphiphilic probes—Section 13.5carbocyanine dyes, cell structure-plasma membrane, DiI, fluorescence microscopy/fluorescence imaging, fluorescent lipid dyes, fluorometer
Molecular Probes HandbookIntroduction to membrane probes—Section 13.1cell structure-plasma membrane, fast-response probes, fluorescence microscopy/fluorescence imaging, fluorometer, ion channels, live-cell imaging, membrane potential, slow-response probes
Molecular Probes HandbookFatty acid analogs and phospholipids—Section 13.2cell structure-plasma membrane, fluorescence microscopy/fluorescence imaging, fluorescent fatty acid analogs, fluorescent lipid dyes, fluorometer
Molecular Probes HandbookDialkylcarbocyanine and dialkylaminostyryl probes—Section 13.4carbocyanine dyes, cell structure-plasma membrane, DiI, fluorescence microscopy/fluorescence imaging, fluorescent lipid dyes, fluorometer
Molecular Probes HandbookProbes for neurotransmitter receptors—Section 16.2brain hormones, cell structure-plasma membrane, fluorescence microscopy/fluorescence imaging, fluorescent dyes, receptor binding
Molecular Probes HandbookProbes for following receptor binding and phagocytosis—Section 16.1cell structure-plasma membrane, fluorescence microscopy/fluorescence imaging, fluorescent dyes, internalization, membrane trafficking, phagocytosis, receptor binding, vesicle
Molecular Probes HandbookProbes for tubulin and other cytoskeletal proteins—Section 11.2cell structure-tubulin, cytoskeleton, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins
Molecular Probes HandbookProbes for actin—Section 11.1cell structure-actin, cytoskeleton, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins
Molecular Probes HandbookProbes useful at near-neutral pH—Section 20.2BCECF, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, pH detection, pH indicators, SNARF
Molecular Probes HandbookProbes useful at acidic pH—Section 20.3flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes HandbookpH indicator conjugates—Section 20.4flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes HandbookOverview of pH indicators—Section 20.1flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes HandbookNucleic acid stains—Section 8.1cell cycle, cell structure-nucleus, cell structure-plasma membrane, cell viability, DAPI, DNA binding dyes, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, membrane permeability, RNA binding dyes, SYTO, SYTOX
Molecular Probes HandbookTracers for membrane labeling—Section 14.4cell structure-plasma membrane, cell tracking, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, live-cell imaging
Molecular Probes HandbookViability and cytotoxicity assay reagents—Section 15.2cell health, cell viability and function, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, microplate reader
Molecular Probes HandbookViability and cytotoxicity assay kits for diverse cell types—Section 15.3cell health, cell viability and function, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, microplate reader
Molecular Probes HandbookAssays for apoptosis and autophagy—Section 15.5apoptosis, autophagy, fluorescent dyes, fluorescence microscopy/fluorescence imaging, flow cytometer/flow cytometry
Molecular Probes HandbookZenon Technology: Versatile reagents for immunolabeling—Section 7.3antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, secondary detection, Zenon
ProtocolTO-PRO-3 Staincell structure, imaging
ProtocolSYTOX Green Nucleic Acid Staincell structure, imaging
ProtocolSYTO 82 Nuclear Staincell structure, imaging
ProtocolSYTO 59 Nuclear Staincell structure, imaging
ProtocolReadyProbes Cell Viability Imaging Kit, Blue/Redcell viability, imaging
ProtocolReadyProbes Cell Viability Imaging Kit, Blue/Greencell viability, imaging
ProtocolPharmacological induction of apoptosis with camptothecincell vitality, flow cytometry, high content analysis, imaging, microplate
ProtocolNucRed Live 647 ReadyProbes Reagentcell structure, imaging
ProtocolNucRed Dead 647 ReadyProbes Reagent for viabilitycell viability, imaging
ProtocolNucRed Dead 647 ReadyProbes Reagent for fixed cellscell structure, imaging
ProtocolNucGreen Dead 488 ReadyProbes Reagent for viabilitycell viability, imaging
ProtocolNucGreen Dead 488 ReadyProbes Reagent for fixed cellscell structure, imaging
ProtocolNucBlue Live ReadyProbes Reagentcell structure, imaging
ProtocolNucBlue Fixed Cell ReadyProbes Reagentcell structure, imaging
ProtocolLIVE/DEAD Yeast Viability Kitcell viability, imaging
ProtocolLIVE/DEAD Viability/Cytotoxicity Kit for mammalian cellscell viability, imaging
ProtocolLIVE/DEAD Sperm Viability Kit for imagingcell viability, imaging
ProtocolLIVE/DEAD Cell Imaging Kit (488/570)cell viability, imaging
ProtocolLIVE/DEAD BacLight Bacterial Viability Kitcell viability, imaging
ProtocolLIVE BacLight Bacterial Gram Stain Kitcell viability, imaging
ProtocolImmunofluorescent staining of intracellular antigens on cultured cellsfluorescence microscopy/fluorescence imaging, general ab staining, imaging, immunofluorescence (IF)
ProtocolImage-iT DEAD Green Kitcell viability, imaging
ProtocolImmunohistochemical staining of formalin-fixed paraffin-embedded tissuesgeneral ab staining, imaging, immunohistochemistry (IHC)
ProtocolIHC frozen tissue—Indirect method (purified)general ab staining, imaging, immunohistochemistry (IHC)
ProtocolIHC frozen tissue—Indirect method (biotin)general ab staining, imaging, immunohistochemistry (IHC)
ProtocolIHC frozen tissue—Direct methodgeneral ab staining, imaging, immunohistochemistry (IHC)
ProtocolIHC FFPE Tissue Trypsin Digestion—Direct Methodgeneral ab staining, imaging, immunohistochemistry (IHC)
ProtocolIHC FFPE tissue trypsin digestion antigen retrieval—Indirect methodgeneral ab staining, imaging, immunohistochemistry (IHC)
ProtocolIHC FFPE tissue low pH antigen retrieval—Indirect methodgeneral ab staining, imaging, immunohistochemistry (IHC)
ProtocolIHC FFPE tissue low pH antigen retrieval—Direct methodgeneral ab staining, imaging, immunohistochemistry (IHC)
ProtocolIHC FFPE tissue high pH antigen retrieval—Indirect methodgeneral ab staining, imaging, immunohistochemistry (IHC)
ProtocolIHC FFPE tissue high pH antigen retrieval—Direct methodgeneral ab staining, imaging, immunohistochemistry (IHC)
ProtocolICC unfixed cells—Direct methodgeneral ab staining, imaging
ProtocolICC methanol fixed cells—Indirect methodgeneral ab staining, imaging
ProtocolICC methanol fixed cells—Direct methodgeneral ab staining, imaging
ProtocolICC formaldehyde fixed, permeabilized cells—Indirect methodgeneral ab staining, imaging
ProtocolICC formaldehyde fixed, permeabilized cells—Direct methodgeneral ab staining, imaging
ProtocolICC formaldehyde fixed cells—Indirect methodgeneral ab staining, imaging
ProtocolICC formaldehyde fixed cells—Direct methodgeneral ab staining, imaging
ProtocolHoechst 33342 for imagingcell structure, imaging
ProtocolHoechst 33342 for HCA instrumentscell structure, high content analysis
ProtocolHCS NuclearMask Red Staincell structure, high content analysis
ProtocolHCS NuclearMask Deep Red Staincell structure, high content analysis
ProtocolHCS NuclearMask Blue Staincell structure, high content analysis
ProtocolHCS Mitochondrial Health Kitcell viability, high content analysis
ProtocolHCS LIVE/DEAD Green Kit using Hoechst 33342cell viability, high content analysis
ProtocolHCS LIVE/DEAD Green Kit using HCS NuclearMask Deep Redcell viability, high content analysis
ProtocolHCS CellMask Stainscell structure, high content analysis
ProtocolDAPI for fluorescence imagingcell structure, imaging
ProtocolDAPI for HCA instrumentscell structure, high content analysis
ProtocolColorimetric FFPE—trypsin digestioncolorimetric staining, general ab staining, imaging
ProtocolColorimetric FFPE—Low pH antigen retrievalcolorimetric staining, general ab staining, imaging
ProtocolColorimetric FFPE—High pH antigen retrievalcolorimetric staining, general ab staining, imaging
ProtocolClick-iT Plus EdU imaging kitscell proliferation, imaging
ProtocolClick-iT EdU imaging kitscell proliferation, imaging
ProtocolClick-iT EdU HCS assayscell proliferation, imaging
ProtocolBrdU labeling and detectioncell proliferation, imaging
ProtocolActinRed 555 ReadyProbes Reagentcell structure, imaging
ProtocolActinGreen 488 ReadyProbes Reagentcell structure, imaging
Scientific poster (2006)LipidTOX dyes for adipocyte staining in routine imaging applications cell differentiation, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, lipid detection, lipids, stem cell research, viability
Scientific poster (2006)Cell cycle analysis using microplate cytometry: A comparison of laser and dye combinations Acumen Explorer, cell cycle, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader
Scientific poster (2006)New fluorescent reagents for the imaging-based assays for the analysis of drug-induced perturbations of cellular lipid metabolism ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, lipid detection, lipids, microplate reader, viability
Scientific poster (2006)Utility of 405 nm-excitable dyes in high content screening using an Acumen Explorer Microplate Cytometer Acumen Explorer, apoptosis, cell cycle, cell health, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, violet laser-excited reagents
Scientific poster (2007)Evaluation of click chemistry-based alternative to BrdU antibody labeling in tissue and cultured cells using fluorescence microscopy and flow cytometry antibodies, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, microplate reader, nucleic acid labeling
Scientific poster (2007)A new approach for the detection of intracellular glutathione by fluorescence microscopy and flow cytometry ArrayScan, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, live-cell imaging, microplate reader, viability, violet laser-excited reagents
Scientific poster (2007)Click chemistry-based detection of S-phase adherent cells using automated microscopy and image analysis Alexa Fluor, antibodies, ArrayScan, cell cycle, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader
Scientific poster (2007)Click catalyzed nucleic acid labeling as a novel replacement for BrdU antibody-based cell proliferation assay Alexa Fluor, antibodies, antibody labeling, cell cycle, cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling
Scientific poster (2007)BacMam gene delivery of targeted fluorescent proteins, Organelle Lights for HCS BacMam technology, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, live-cell imaging, nanocrystals, Qdot
Scientific poster (2008)Living molecular probes for the next generation BacMam technology, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, ion flux/ion channels, ion indicators, live-cell imaging
Scientific poster (2008)Quantum dot (Qdot) labeling of gene expression in fresh frozen brain tissue using high-throughput in situ hybridization in situ hybridization, brightfield microscopy, confocal microscopy,fluorescence microscopy/fluorescence imaging, fluorescent dyes, gene expression, high content analysis, nanocrystals, neuroscience, Qdot, tissue
Scientific poster (2008)Characterization of DNA content, cyclin B1 and phosphorylated histone H3 with direct S-phase using EDU incorporation in multiparameter testing of cell lines with cell cycle blocking agents Alexa Fluor, antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis
Scientific poster (2008)Cell-based potassium ion channel screening with the FluxOR Assay BacMam technology, cell counting, Countess, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader
Scientific poster (2008)Novel Click-IT TUNEL Assay for detection of cell death Alexa Fluor, antibodies, apoptosis, Click-iT, cytotoxicity, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, high content analysis, immunocytochemistry (ICC), viability
Scientific poster (2008)Effects of the thymidine analogues EdU and BrdU on cell viability and cycle progression Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, viability
Scientific poster (2008)Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry Alexa Fluor, antibodies, ArrayScan, cell cycle, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, high content analysis, microplate reader, nucleic acid labeling, nucleic acid quantitation
Scientific poster (2008)FluxOR, a universal potassium channel assay: Screening BacMam-delivered and stably expressed targets BacMam technology, drug discovery, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader
Scientific poster (2008)ThiolTracker Violet dye, a new 405 nm excitable fluorescent probe for detecting intracellular thiols in imaging-based assays ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, glutathione, high content analysis, microplate reader, protein labeling, violet laser-excited reagents
Scientific poster (2008)Modified tumor cell protein expression and modification under physiological oxygen Alexa Fluor, cancer, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, physiological oxygen level, protein labeling
Scientific poster (2008)A new 405 nm excitable fluorescent probe of intracellular thiols for imaging and flow cytometric assays ArrayScan, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, high content analysis, microplate reader, thiol modification, violet laser-excited reagents
Scientific poster (2008)Detection of DNA synthesis by automated microscopy and image analysis: Comparison of BRDU method and a new click chemistry-based EDU method Alexa Fluor, antibodies, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, high content analysis, microplate reader, nucleic acid labeling
Scientific poster (2009)Cell-based assays for predictive hepatotoxicity measurements using high content imaging Alexa Fluor, ArrayScan, cell structure-plasma membrane, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, high content analysis, microplate reader, nucleic acid quantitation, viability
Scientific poster (2009)A non-radioactive method for detecting nascent protein synthesis by high-content imaging utilizing click chemistry Alexa Fluor, apoptosis, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, protein detection
Scientific poster (2009)Monitoring mitotic cells and DNA content by automated imaging and analysis Alexa Fluor, cell cycle, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling, nucleic acid quantitation, viability
Scientific poster (2009)Dual pulse labeling using a new thymidine analog 5-ethynyl-2'-deoxyuridine (EdU) to detect alterations in S-phase progression by fluorescence microscopy and flow cytometry Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Scientific poster (2009)High content imaging of endocytosis & phagocytosis using pHrodo conjugates Alexa Fluor, ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, pH detection, phagocytosis, viability
Scientific poster (2009)Click chemistry-based detection of nascent RNA synthesis using high content imaging and fluorescence microscopy Alexa Fluor, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, nucleic acid labeling, viability
Scientific poster (2009)High content analysis of cytotoxicity by laser scanning fluorescence microplate cytometry Alexa Fluor, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, viability
Scientific poster (2009)Non-radioactive targeting of multiple classes of protein post-translational modifications (PTMs) with click chemistry cell signaling, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein detection, protein enrichment, protein labeling, western blotting
Scientific poster (2009)High content imaging and analysis of mitotoxicity and cytotoxicity in fixed cells cell structure-mitochondria, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, viability
Scientific poster (2009)Quantitative analysis of genotoxicity and cytotoxicity to DNA damaging agents using high-content imaging Alexa Fluor, antibodies, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling, nucleic acid quantitation, viability
Scientific poster (2009)Metabolic labeling and click chemistry detection of stem cell markers Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein labeling, stem cell research
Scientific poster (2009)Cell-based potassium ion channel screening with BacMam using the FluxOR Assay BacMam technology, drug discovery, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader
Scientific poster (2009)Click chemistry-based assays for fluorescence microscopy and high content imaging Alexa Fluor, apoptosis, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid detection, protein detection, viability
Scientific poster (2010)Simultaneous analysis of cell death mechanisms and oxidative stress using Molecular Probes next generation reagents for imaging and flow cytometry apoptosis, autophagy, caspase substrates, flow cytometry/flow cytometer, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, oxidative stress
Scientific poster (2010)High content imaging of autophagy antibodies, ArrayScan, autophagy, BacMam technology, cell signaling, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging, microplate reader
Scientific poster (2010)Oxidative stress-induced protein modification: Application of clickable linoleic acid analogs Alexa Fluor, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, high content analysis, microplate reader, oxidative stress, protein detection, protein enrichment
Scientific poster (2010)Antibody- and fluorescent protein-based approaches to measuring autophagy in mammalian cells by fluorescence microscopy Alexa Fluor, antibodies, autophagy, BacMam technology, cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging
Scientific poster (2010)Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry and imaging Alexa Fluor, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF), immunophenotyping, stem cell research, violet laser-excited reagents
Scientific poster (2010)Novel tools to enable high resolution gene expression by investigating the nascent transcriptome Alexa Fluor, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gene expression, high content analysis, magnetic beads, nucleic acid labeling, PCR/qPCR, viability
Scientific poster (2010)Click chemistry-based enrichment and identification of nascent and post-translationally modified proteins cells, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein detection, protein enrichment, protein labeling
Scientific poster (2010)Autophagosomal accumulation perturbs Golgi structure without affecting other organelles: Implications for autophagosome biogenesis Alexa Fluor, antibodies, autophagy, BacMam technology, cell structure-golgi, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging
Scientific poster (2010)A new generation of cellular imaging probes for neurosciences research autophagy, BacMam technology, CellLight, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels, ion indicators
Scientific poster (2010)ABfinity antibodies: A novel approach to antibody development Alexa Fluor, antibodies, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gene expression, immunocytochemistry (ICC), western blotting
Scientific poster (2010)NA-Fluor Influenza Neuraminidase Assay Kit: Standardization of the classic MUNANA-based neuraminidase activity assay for monitoring antiviral resistance in influenza drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, influenza, microbiology, microplate reader
Scientific poster (2010)Simultaneous analysis of cell death mechanisms and oxidative stress using live cell fluorescence microscopy apoptosis, ArrayScan, BacMam technology, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging, microplate reader, oxidative stress
Scientific poster (2010)New fluorescent probes for live-cell imaging autophagy, cell cycle, CellLight, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging, pH detection, phagocytosis
Scientific poster (2010)The NA-XTD Influenza Neuraminidase Assay Kit: Extended-glow chemiluminescence assay system for highly sensitive influenza neuraminidase assays chemiluminescence, drug discovery,fluorescence microscopy/fluorescence imaging, high content analysis, influenza, microbiology, microplate reader
Scientific poster (2010)Non-cytotoxic near-IR DNA stain for cell cycle analysis in living cells cell cycle, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, live-cell imaging, nucleic acid labeling, viability
Scientific poster (2010)Click chemistry enrichment of cell surface glycoproteins cells, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein detection, protein enrichment, protein labeling
Scientific poster (2010)Identification and characterization of O-GlcNAc modification of galectin-1 in mesenchymal stem cells using click chemistry Alexa Fluor, BacMam technology, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, immunocytochemistry (ICC), protein enrichment, protein labeling, western blotting
Scientific poster (2010)BacMam gene expression for functional and imaging applications BacMam technology, cell cycle, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels
Scientific poster (2010)Click chemistry-based universal enrichment columns for global identification of azide-modified proteins Alexa Fluor, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein detection, protein enrichment, protein labeling
Scientific poster (2010)Dual pulse labeling using EdU and BrdU to monitor alterations in cellular proliferation by fluorescence microscopy and flow cytometry Alexa Fluor, cell cycle, cell proliferation, Click-iT, drug discovery, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
Scientific poster (2010)A universal click chemistry-based enrichment column for the identification of multiple subclasses of PTM-modified proteins and newly synthesized proteins Alexa Fluor, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein detection, protein enrichment, protein labeling
Scientific poster (2011)Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health ArrayScan, cell health, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress, viability
Scientific poster (2011)Acoustic cytometric identification and initial characterization of limbal stem cells isolated from human cornea Attune/Attune NxT, cell culture/expansion, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, PCR/qPCR, stem cell research, tissue, violet laser-excited reagents
Scientific poster (2011)Simultaneous analysis of cell death mechanisms and oxidative stress using high content imaging Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress
Scientific poster (2011)Primary human cells from Life Technologies: Offerings, scale and applications in HCS and HTS formats Alexa Fluor, antibodies, autophagy, BacMam technology, cell proliferation, cell signaling, Click-iT, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis
Scientific poster (2011)Validation of high content imaging and analysis assays for cell health and cytotoxicity antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, cell health, cell proliferation, CellLight, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, pH detection
Scientific poster (2011)The next step in the analysis of lymphocyte proliferation Alexa Fluor, antibodies, Attune/Attune NxT, cell proliferation, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, live-cell imaging, violet laser-excited reagents
Scientific poster (2011)The next generation of cell-based imaging assays for apoptosis, autophagy and oxidative stress from Molecular Probes Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress
Scientific poster (2011)NA-Fluor and NA-XTD Influenza Neuraminidase Assays for neuraminidase quantitation and inhibition assays chemiluminescence, drug discovery,fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, influenza, microbiology, microplate reader
Scientific poster (2012)Cell-based analysis of oxidative stress, lipid peroxidation and lipid peroxidation-derived protein modifications using fluorescence microscopy flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, high content analysis, lipid peroxidation, microplate reader, oxidative stress, viability
Scientific poster (2013)Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health apoptosis, ArrayScan, caspase substrates, cell health, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, microplate reader, oxidative stress
Scientific poster (2013)Cells coming to life: Tools for visualizing immune response Attune/Attune NxT, endocytosis/phagocytosis, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, immunophenotyping, live-cell imaging, pH detection
Scientific poster (2013)Illuminating endocytosis with targeted pH-sensitive fluorescent compounds Alexa Fluor, ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, pH detection, phagocytosis, viability
Scientific poster (2014)New fluorescent probes and sensors for visualizing endocytosis, lysosomal dynamics and autophagy autophagy, cell structure-lysosomes, endocytosis/phagocytosis, autophagy, fluorescence microscopy/fluorescence imaging, high content analysis, microplate reader
Scientific poster (2015)Colorimetric dual labeled EdU/BrdU technology demonstrates contextual information and dynamics of proliferation in tissue apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC)
Scientific poster (2015)Visualizing the life and death of cells: novel probes and platforms apoptosis, ArrayScan, autophagy, cell death, CellInsight, fluorescence microscopy/fluorescence imaging, high content analysis
Scientific poster (2015)Intracellular detection of hypoxia in live cells EVOS, fluorescence microscopy/fluorescence imaging, hypoxia, EVOS, onstage incubator
Scientific poster (2016)High content screening in MCF7 and MDA-MB231 cells show differential responses depending on oxygen levels and mechanistic readout for viabilitycell health, cell viability and function, drug discovery, high content analysis, high throughput screening
Scientific poster (2016)Correlating internalization and potency to accelerate antibody discovery and developmentbrightfield microscopy, colorimetric, endocytosis and phagocytosis, EVOS,fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunohistochemistry (IHC)
Scientific poster (2016)A novel non-cytotoxic fluorescent dye for cell proliferation analysis in flow cytometry Attune/Attune NxT, cell health, cell proliferation, CellTrace, EVOS, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging
Scientific poster (2016)Incorporating histological analysis into existing high content imaging antibodies, ArrayScan, brightfield microscopy, fluorescence microscopy/fluorescence imaging, high content analysis, immunohistochemistry (IHC)
Scientific poster (2016)Click colorimetric EdU proliferation and TUNEL: Click chemistry for brightfield microscopy apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC)
Scientific poster (2017)Matching refractive index of mounting media improves axial resolution and image quality in 3D biological samples antifades, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, fluorescent proteins, imaging sample preparation, mountant, onstage incubator, photobleaching
Scientific poster (2017)Hypoxia measurements in live and fixed cells using fluorescence microscopy and high-content imaging EVOS, fluorescence microscopy/fluorescence imaging, high content analysis, hypoxia, live-cell imaging, onstage incubator
Tutorial

1.2 Fixation–Fixed cell imaging: 5 steps for publication-quality images  
The next step of tissue preparation is fixation. Fixation refers to a chemical means of killing and preserving cells in a particular physiological state, and in many cases, to preserve morphology. Proper fixation equals preservation of target

fixation, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF)
Tutorial

4.1 Mounting media–Fixed cell imaging: 5 steps for publication-quality images  
Using the right mounting media can impact your experiment. Be sure to choose the right type of mountant for your set-up

antifade mounting media, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), mounting media
Tutorial

3.2 Secondary antibody optimization–Fixed cell imaging: 5 steps for publication-quality images  
Secondary antibody detection protocols also need to be optimized for each primary antibody used

antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), secondary antibodies, secondary antibody protocol, secondary detection
Tutorial

4.2 Photobleaching and antifades–Fixed cell imaging: 5 steps for publication-quality images  
What is photobleaching and how can you prevent it from destroying your sample? Options for antifades are discussed

antifades, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), photobleaching, Prolong Gold, Vectashield
Tutorial

5.5 Ethical considerations–Fixed cell imaging: 5 steps for publication-quality images  
Ethical imaging means trustworthy data, and thus, publishable data. How to treat your samples and data to preserve data integrity is presented

ethical considerations, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, image analysis, immunofluorescence (IF), sample manipulation
Tutorial

5.2 Imaging platforms-software–Fixed cell imaging: 5 steps for publication-quality images  
Taking images on a microscope usually entails having some type of imaging software that aids in taking the image and assists in combining differing colors into one. There are some very important aspects to keep in mind to get a publishable image

FiFI software, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, ImageJ, imaging software, imaging systems, immunofluorescence (IF)
Tutorial

1.1 Culture conditions–Fixed cell imaging: 5 steps for publication-quality images 
The first step in obtaining a good image is tissue preparation. For cultured cells, the cells must have good cell health and morphology, as well as good confluency. Healthy cells equal healthy data

EVOS, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, imaging hardware, imaging systems, immunofluorescence (IF), microscope
Tutorial

1.5 Autofluorescence–Fixed cell imaging: 5 steps for publication-quality images  
The last step in cell preparation is autofluorescence. Cells and tissue can have a certain degree of autofluorescence that can confuse the specific signal, and lower the signal-to-background. Overcoming autofluorescence means greater sensitivity

autofluorescence, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), tissue autofluorescence
Tutorial

5.4 Image analysis with Celleste software–Fixed cell imaging: 5 steps for publication-quality images  
The functionality of the Celleste software reviewed and considerations for processing the image in different software programs are described

Celleste software, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, image analysis, immunofluorescence (IF)
Tutorial

1.4 Blocking–Fixed cell imaging: 5 steps for publication-quality images  
The next step after permeabilization is blocking, and there are a number of blocking techniques. Protein blocking equals specific antibody binding. Dye charge blocking means less non-specific binding

dye charge blocking, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), nonspecific binding, protein blocking
Tutorial

2.2 Primary antibody protocol optimization–Fixed cell imaging: 5 steps for publication-quality images  
Every primary antibody must be optimized separately. There are many protocols available, and it is important to understand a "one size fits all" approach gives inferior results, as every antibody is slightly different. Learn how to approach optimization

antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), primary antibodies, primary antibody protocol
Tutorial

5.1 Imaging platforms-hardware–Fixed cell imaging: 5 steps for publication-quality images 
The fifth step of the process is the actual imaging. To capture top-quality images, you need an imaging platform with top-of-the-line imaging capabilities. Here we review considerations for getting the best image

EVOS, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, imaging hardware, imaging systems, immunofluorescence (IF), microscope
Tutorial

3.5 Dye choice and special concerns–Fixed cell imaging: 5 steps for publication-quality images 
There are many different dyes spanning the visible, far-red, and infrared wavelengths.Considerations for making the right choices for your experiment are presented

dye choice, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, Fluorescence SpectraViewer, immunofluorescence (IF), label choice, multiparametric dye selection
Tutorial

1.3 Permeabilization–Fixed cell imaging: 5 steps for publication-quality images  
The next step is the permeabilization of the cells which is the key to opening intracellular compartments

cell permeabilization, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF)
Tutorial

3.4 Controls–Fixed cell imaging: 5 steps for publication-quality images  
Researchers should conduct all necessary controls to rule out the possibility of non-specific binding or non-specific signal. Types of controls are described. Proper controls will boost your confidence in your final results

fixed-cell imaging, fluorescence microscopy/fluorescence imaging, imaging controls, immunofluorescence (IF), sample controls
Tutorial

5.3 Image capture with EVOS FL Auto 2.0–Fixed cell imaging: 5 steps for publication-quality images  
Here the advantages of using the EVOS FL Auto 2.0 imaging system to capture your images are discussed

EVOS FL Auto 2.0, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, image capture, immunofluorescence (IF)
Tutorial

3.3 Amplification techniques–Fixed cell imaging: 5 steps for publication-quality images  
If the signal is not strong enough using standard secondary detection schemes, you can increase the signal using amplification techniques. This is particularly important for low-expressing antigens, or rare-cell detection in samples. Examples are discussed

biotin and streptavidin, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), signal amplification, tyramide detection
Tutorial

2.1 Primary antibody choice–Fixed cell imaging: 5 steps for publication-quality images  
After preparation, the second step to publishable images is to label the sample, usually involving primary antibodies to your specific targets of interest. The antibody source, the use of direct versus labeled antibodies as well as the validation for specific applications is discussed

antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), primary antibodies
Tutorial

3.1 Secondary antibody choice–Fixed cell imaging: 5 steps for publication-quality images  
Step three of the five steps in making publishable images is to detect the label. That is, to detect with a secondary antibody, for instance, or an amplification technique, as well as to determine what controls to use. Your options are discussed

antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), secondary antibodies, secondary antibody, secondary detection
Tutorial

Labeling a purified antibody: Educational video on how to label your next antibody for imaging or fluorescence 
Walk through the tutorial as Molecular Probes scientists demonstrate the protocol—including all the tips and tricks you'll want to know about for your next antibody labeling experiment. The video features Judie showing Curtis, a chemistry graduate student, how to label his monoclonal antibodies while saving time and maximizing yield.

antibodies, antibody labeling, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF)
Tutorial

HCA Q & A 
A video series that answers common questions about high content analysis and the different Thermo Scientific high content imaging platforms

ArrayScan, CellInsight, high content analysis
Video

Setting up an automated scan routine on the EVOS FL Auto 2 Microscope 
Save time when imaging cells by easily setting up an automated scan routine using the EVOS FL Auto 2 microscope

EVOS, fluorescence microscopy/fluorescence imaging, instrument set-up
VideoCellEvent Caspase 3/7 reagent and apoptosis detection 
HeLa cells were loaded with 50 nM TMRM (red) followed by 5 μM CellEvent Caspase 3/7 Substrate (green). Cells were then treated with 0.5 μM staurosporine to induce apoptosis. TMRM detects the mitochondrial membrane potential and the signal disappears as the apoptosis onsets. CellEvent detects apoptosis, with green signal.
apoptosis, cell health, CellEvent, fluorescence microscopy/fluorescence imaging, live-cell imaging
VideoViability determination of HeLa cells using ReadyProbes Cell Viability Imaging Kit (Blue/Green) 
HeLa cells were loaded with NucBlue Live and NucGreen Dead (using 2 drops per ml) in complete media for 15 minutes at 37C. Staurosporine was then added to a final concentration of 1 µM and images were acquired every 30 min. for 18 hours using EVOS Auto Imaging System. All cells are stained with NucBlue Live, shown with blue nuclei. Over time an increase in the number of dead cells is observed as indicated by the appearance of green nuclei (NucGreen Dead).
cell structure-nucleus, cell viability, fluorescence microscopy/fluorescence imaging, live-cell imaging
VideoAdding oil to objectives on the EVOS FL Auto 2 Microscope 
See how easy it is to add oil to your objective without having to remove your sample when imaging with the EVOS FL Auto 2 Microscope.
EVOS FL Auto 2 Microscope, fluorescence microscopy/fluorescence imaging, instrument set-up
VideoReviewing Cell Images on the EVOS FL Auto 2 Microscope 
Cell images can be saved from the EVOS FL Auto 2 as a single field, scan, time lapse, and z-stack. Easily review your images and metadata or make adjustments to image brightness, contrast, or gamma and resave your images with the new settings.
EVOS FL Auto 2 Microscope, fluorescence microscopy/fluorescence imaging, instrument set-up
VideoUsing the locations tool on the EVOS FL Auto 2 Microscope 
Learn how to create custom locations to use in automated routines for scanning and time lapse experiments on the EVOS FL Auto 2 Cell Imaging System.
EVOS, fluorescence microscopy/fluorescence imaging, instrument set-up
VideoHow to setup vessel maps on the EVOS FL Auto 2 Microscope 
The EVOS FL Auto 2 can accommodate most vessel types and sizes, including slides, multi-well plates, culture flasks, and petri dishes. See how easy it is to setup the EVOS FL Auto 2 for imaging with the vessel maps tool.
EVOS FL Auto 2 Microscope, fluorescence microscopy/fluorescence imaging, instrument set-up
VideoHow to add a scale bar and grid to cell images on the EVOS FL Auto 2 Microscope 
See how easy it is to add a scale bar and grid to your live or captured cell images on the EVOS FL Auto Microscope. You can adjust the grid size, color, and alignment to fit the needs of your experiment.
EVOS FL Auto 2 Microscope, fluorescence microscopy/fluorescence imaging, instrument set-up
VideoCreate z-stacks using the EVOS FL Auto 2 Microscope 
Capturing images at different Z-planes can be a powerful tool in fluorescence microscopy. This capability can reveal conditions not seen with standard widefield imaging and easier than ever to use with the EVOS FL Auto 2 microscope.
EVOS FL Auto 2 Microscope, fluorescence microscopy/fluorescence imaging, instrument set-up
VideoLIVE DEAD Cell Imaging kit on the EVOS Auto Imaging System 
Time course of cell death visualized using the LIVE/DEAD Cell Imaging kit (R37601). The LIVE/DEAD Cell Imaging kit is based on a cell-permeable dye (calcein, AM) that stains live, viable cells bright green and a cell-impermeable red marker that only stains dead and dying cells, which are characterized by compromised cell membranes. Labeled U-2 OS cells were treated 1 μM staurosporine and fluorescence images in the FITC and TexasRed channels were acquired every 5 minutes over 14 h on the EVOS Auto Imaging System using a 20x objective.
cell viability, fluorescence microscopy/fluorescence imaging, ion channels, ion flux, ion indicators, live-cell imaging
VideoCellTracker Violet reagent and mitosis 
U-2 OS cells were transduced with CellLight Tubulin-GFP and Cellular Lights Actin-RFP. The following day cells were labeled with 5uM CellTracker Violet BMQC for 30 minutes at 37C in complete media and washed in fresh media. Images were taken every 5 minutes for 16 hours.
cell structure-all, cell tracking, fluorescence microscopy/fluorescence imaging, live-cell imaging
VideoWound healing and cadherin-4 expression 
Murine glioma cells were transfected with a DsRed-cadherin-4 fusion, which plays a critical role in cell migration during wound repair, or a GFP control plasmid, then mixed with wild-type cells and seeded in a 24-well plate. After scratch wounding, cell migration was followed over a 21 h period on the EVOS Cell Imaging System equipped with an EVOS Onstage Incubator using a 10X phase objective. Images were captured at an interval of about 10 minutes (116 images total). Video courtesy of Paolo Malatesta, PhD., IRCCS Azienda Ospedaliera Universitaria San Martino -- IST, Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
EVOS, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging, wound healing
VideoCompilation of live cell imaging videos using Invitrogen fluorescent reagents 
This video demonstrates novel product brands from Invitrogen for live cell imaging include CellLight targeted fluorescent proteins, CellROX reagents for oxidative stress, CellEvent caspase 3/7 detection reagent, and many more fluorescent dyes and probes.
cell structure-mitochondria, CellEvent, CellLight, CellROX, fluorescence microscopy/fluorescence imaging, live-cell imaging, oxidative stress
VideoClick-iT Plus TUNEL apoptosis assays 
The Click-iT Plus TUNEL assay detects apoptotic cells in tissue and cultured cell samples through the use of a small, highly specific labeling moiety and a bright fluorescent dye. After incorporation of the labeling moiety into DNA fragments, detection is achieved through a catalyzed "click" reaction using conditions mild enough to preserve the fluorescent signal from GFP or RFP.
apoptosis, Click-iT, fluorescence microscopy/fluorescence imaging
VideoWashing your sample 
Molecular Probes School of Fluorescence explains the wash step common to many cellular imaging protocols.
fluorescence microscopy/fluorescence imaging, imaging sample preparation
VideoZ Stack image of HeLa cells labeled with CellLights reagents 
A series of images were captured on the EVOS FL Auto Cell Imaging System. Creating a Z-stack from these images allowed the observation of cellular cytoskeletal changes, which can be indicative of the loss of cell health. Methods HeLa cells grown in MatTek 6-well glass bottom culture plates were transduced with CellLights Tubulin-GFP and CellLight Mitochondria-RFP overnight at 37oC. The following day, NucBlue Live reagent (2 drops/mL) was added to the cultures. Cells were then imaged on an EVOS FL Auto Cell Imaging System with 100x oil immersion objective using the Z-stack function. The step size was set using the Nyquist formula and performed at 0.366 μm.
cell health, cell structure-all, CellLight, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging
VideoHow to site specifically label your antibody using SiteClick technology 
This video explains how to easily and site specifically label an antibody using an enzymatic and Click chemistry approach. This method can be applied to any intact IgG antibody and requires no antibody engineering or complex methodology. Unlike classic antibody conjugation techniques this breakthrough SiteClick technology allows for antibody conjugation with complete confidence that the label will not directly interfere with the antibody binding domain.
antibodies, antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, Qdot, western detection
VideoMounting a Coverslip – Molecular Probes School of Fluorescence 
Molecular Probes School of Fluorescence explains step-by-step how to mount a coverslip properly for cellular imaging. Formulations of mounting media that can add favorable properties such as optimizing the refractive index to match that of glass, preventing photobleaching, or preserving samples for long-term storage are widely available.
fluorescence microscopy/fluorescence imaging, imaging sample preparation
VideoDetection of oxidative stress with CellROX Green in U2-OS cells 
U2-OS cells were plated at 200,000 cells per dish on a 35 mm glass bottom dish (MatTek) and cultured overnight. The cells were rinsed once and loaded with 5 µM CellROX Green Reagent (Cat. No. C10444) and 100 nM TMRM in Live Cell Imaging Solution (LCIS, Cat. No. A14291DJ) for 15 minutes at 37 degrees C. After loading, cells were imaged live with no washing every 20 seconds for seventy minutes on the DeltaVision Core inverted microscope at 37 degrees C. Menadione was added to a final concentration of 100 uM after ten minutes of baseline acquisition. The time lapse data shows the loss of signal from TMRM in the red channel as mitochondrial function decreases, concomitant with the onset of a nuclear signal in the green channel as CellRox Green is oxidized and the reagent migrates to the nucleus to generate a fluorogenic response as the active form of the dye binds to DNA.
CellROX, fluorescence microscopy/fluorescence imaging, live-cell imaging, oxidative stress
VideoTime-lapse of cell migration and apoptosis during angiogenesis 
Angiogenesis pseudo-tube formation and apoptosis shown with time-lapse imaging. The time-lapse images were captured and assembled using an EVOS FL Auto Imaging System equipped with the EVOS Onstage Incubator.
angiogenesis, apoptosis, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, live-cell imaging, onstage incubator
VideoMitochondrial dynamics through cell division 
U2OS cells were transduced with CellLight Mito-RFP and imaged every 5 minutes for 16 hours. Extensive mitochondrial motility is seen throughout mitosis and as the cell regains it's pre mitotic shape following mitosis.
cell structure-mitochondria, CellLight, fluorescence microscopy/fluorescence imaging, live-cell imaging
VideoClick-iT Plus EdU Proliferation Assay 
Measuring DNA synthesis is the most precise way to detect changes in cell proliferation. Image-based proliferation assays generate spatial and temporal results that can not be detected with other methods. This video describes and compares two of the most referenced image-based proliferation technologies.
cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging
VideoTime-lapse with Qtracker 655 cell labeling reagent 
Time-lapse image of live cells labeled with Qtracker reagents. Adherent HeLa cells were incubated with Qtracker VIVID 655 Cell Labeling Kit (Cat. No.Q25021MP) at 10 nM for one hour. Images were collected over 90 min using a Zeiss LSM 710 confocal microscope and are shown pseudo-colored magenta.
fluorescence microscopy/fluorescence imaging, live-cell imaging
VideoTips and tricks: Phospho Immunofluorescence cell analysis antibodies, cell imaging, fluorescence microscopy/fluorescence imaging, fixed-cell imaging, immunofluorescence (IF)
VideoThe secret to solving the most common problem in IF experiments cell analysis antibodies, cell imaging, fluorescence microscopy/fluorescence imaging, fixed-cell imaging, immunofluorescence (IF)
VideoImmunohistochemistry—Tips and tricks for weak or no stain Immunohistochemistry application, offers a spatial visualization and location of an antigen in tissue sections antibodies, brightfield microscopy, colorimetric, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC), secondary detection
VideoImmunohistochemistry—Tips and tricks for high background Immunohistochemistry application offers a spatial visualization and location of an antigen in tissue sections. This video offers troubleshooting tips for issues falling into the "high background" category for IHC antibodies, brightfield microscopy, colorimetric, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC), secondary detection
VideoBeginner's guide to macro writing: Celleste Image Analysis Software brightfield microscopy, Celleste, fluorescence microscopy/fluorescence imaging, imaging analysis
VideoRegions of interest: Celleste Image Analysis Software brightfield microscopy, Celleste, fluorescence microscopy/fluorescence imaging, imaging analysis
VideoCell and object counting: Celleste Image Analysis Software brightfield microscopy, Celleste, fluorescence microscopy/fluorescence imaging, imaging analysis
VideoBatch processing: Celleste Image Analysis Software brightfield microscopy, Celleste, fluorescence microscopy/fluorescence imaging, imaging analysis
WebinarFixed cell imaging—Five steps for publication-quality images 
With over 40 years dedicated to cell imaging research, we offer long-proven tools and protocols to help confidently create quality cell images the first time. This on demand webinar covers the 5 essential steps to getting great images.
antibodies, blocking, Celleste, dyes, EVOS FL Auto 2.0, fixation, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), sample detection, sample labeling, sample preparation, signal amplification
WebinarLearn to choose the right fluorophore when designing experiments  
The choice of fluorophore is one of the first important decisions to make in developing an experiment. Fluorophores are compounds that emit light at a specific wavelength when they have been excited at another, lower wavelength. Join our webinar and explore: How to choose the best organic dye for an assay Quantum dots and how they compare to other dyes When to use a phycobiliprotein like R-PE or APC When to use fluorescent proteins like GFP How to choose a suitable dye to match your instrument In addition, we will explore the basic characteristics, strengths, and weaknesses of the various fluorophores to help you choose and develop the best assay for your needs.
antibodies, antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins
WebinarA practical approach to antibody labeling 
The growing number of fluorophores available makes labeling your own antibodies a tempting proposition. But with many antibody labeling solutions available, selecting the best option can be a daunting task. In this webinar we will: Provide an overview of our antibody labeling kits Offer guidance on which methods are ideal for specific applications and experiments Provide tips and tricks to optimize your labeling protocol
antibodies, antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF)
WebinarAn introduction to immunofluorescence staining of cultured cells  
In this webinar, we discuss the steps of an immunofluorescent staining protocol including material list, common variations, and necessary controls. We'll also provide a simple troubleshooting guide and examine how to avoid common pitfalls. Presented by Jason Kilgore, Technical Support Specialist, Thermo Fisher Scientific.
antibodies, antibody labeling,antifades, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF)
WebinarBasic techniques in autophagy research  
This webinar will introduce you to a series of analytical tools and techniques to help you identify and interrogate key features of autophagy. Topics to be covered include: Tips and tricks for selecting the right tools and achieving the best results Fluorescent proteins and antibodies used to analyze both live and fixed cells Analysis steps using a variety of multiplexing options, with quantitative methods for image analysis or fluorescence intensity measurement
autophagy, cell health, fluorescence microscopy/fluorescence imaging, fluorescent proteins, high content analysis, immunofluorescence (IF)
WebinarThe meaning of life at the cellular level: detecting apoptosis with fluorescence 
In this webinar, Thermo Fisher Scientific scientists will demonstrate several solutions using fluorescent probes to study key components of the apoptotic machinery. The presentation includes: An overview of the features of apoptosis Key parameters that can be measured to assay apoptosis A comprehensive guide to available labeling and detection technologies for apoptosis research Tips and tricks to best implement those technologies
apoptosis, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent proteins
WebinarThe meaning of life at the cellular level: Visualizing membrane trafficking 
Membrane trafficking underlies the acquisition and release of cellular material as well as the transport of macromolecules from the site of cellular synthesis to their functional location. Thermo Fisher Scientific offers a number of solutions for researchers using fluorescent probes to study key components of the endocytotic and secretory pathway. This webinar will: Provide an overview of the processes involved in membrane trafficking, along with strategies to investigate them using fluorescence microscopy Offer a comprehensive guide to available labeling and detection technologies for membrane trafficking research Provide tips and tricks on how to best implement those technologies
endocytosis, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging, membrane trafficking, particles
WebinarA comparison of basic immunofluorescent labeling strategies 
In this free webinar, we will compare different immunofluorescent labeling strategies exploring the pros and cons of each method. You will learn when the use of a direct conjugate is appropriate and when amplification techniques can be utilized. We'll also present a simple decision tree to aid in determining the best method for each situation.
Alexa Fluor, antibodies, antibody labeling, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, immunocytochemistry (ICC), immunofluorescence (IF)
WebinarBreathing new life into hypoxia research 
Although the significance of hypoxia in biological processes is well known, creating model systems with accurate control of hypoxic conditions is extremely difficult without access to elaborate systems that allow precise control and maintenance of temperature, humidity, and gases (CO2 and O2) during an experiment. In this webinar, we will discuss: Overview of hypoxia in human diseases Classical methods for setting up hypoxic conditions Novel instruments and reagents for imaging cells in hypoxic conditions
EVOS, fluorescence microscopy/fluorescence imaging, high content analysis, hypoxia, onstage incubator
WebinarThe meaning of life at the cellular level: Probing viability with fluorescence 
This webinar will provide an overview of features of healthy and unhealthy cells as well as describing key parameters that can be measured to assay cell viability. The webinar also offers a comprehensive guide to available labeling and detection technologies for cell health research as well as tips and tricks on how to best use them.
cell health, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, viability
WebinarIntroduction to basic cytoskeleton labeling and detection 
The cytoskeleton is a key component of mammalian cells, providing the framework for cell migration and intracellular transport, furthermore the cytoskeleton regulates cell size and shape as well as important processes such as mitosis and endocytosis. We offer a number of solutions for researchers using fluorescent probes to study the cytoskeleton. This webinar will provide an overview of the structures that comprise the cytoskeleton and important experimental parameters. The webinar also offers a comprehensive guide to available labeling and detection technologies for cytoskeletal research as well as tips and tricks on how to best use them. These tools include those for live-cell imaging fluorescent dyes, antibodies and the BacMam gene delivery platform.
cell structure-actin, cytoskeleton, endocytosis, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins
WebinarFrom the hood to the microscope; Revolutionizing cell-based imaging 
Join us for an interactive, educational webinar that showcases some of the latest advancements in cell-based research and imaging. Learn as we explore how to obtain superior results through the careful selection of reagents and the optimization of your imaging workflow— from growing cells on suitable surfaces through to image capture. Particular attention will be given to growing and monitoring cells for imaging, fluorescent labeling of live cells, critical considerations for time-lapse imaging and optimizing live cell imaging. Topics include: Choose a suitable imaging culture vessel for studying cell growth and viability using bright-field microscopy Label cells to maximize signal-to-noise for fluorescence imaging Prepare culture conditions and capture images without losing temporal data for time-lapse imaging Achieve hypoxic culture conditions by modifying gas conditions or employing cell spheroid cultures
cell health, EVOS, FLoid, fluorescence microscopy/fluorescence imaging, hypoxia, live-cell imaging, onstage incubator
White paper (2015)Accuracy and precision with the Countess II FL Automated Cell Counterautomated cell counter, brightfield microscopy, cell counting, Countess