Immunology at Work Resource Center

Watch & Learn

Watch our learning series for on-demand tutorials and webinars to receive firsthand knowledge of the latest technologies to accelerate your Immunology Research.

Cell-based Assays to Functionally Characterize Immunotherapy and the Immune Response to Viral Infection

With accelerating development and approval of cell therapies, biologic drugs, and antiviral treatments comes a need for more robust and reliable cell-based assays and validated analysis systems. In this seminar, a variety of techniques for immune cell isolation, expansion, and characterization will be demonstrated in cell-based immunotherapy and virology models using powerful cytometric instrumentation. Special focus will be given to immune effector function assays to characterize the potency and specificity of CAR-T and natural killer cells. Additionally, scalable screening methods based on recent innovations in high-throughput flow cytometry, partnered with regulatory-compliant acquisition and analysis software, will provide valuable additions to your portfolio of assays for immunotherapy and virology research.

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Cell-Based Assays and Analysis in Antimicrobial Research

Translational research focused on drug-resistant and emerging pathogens requires robust and reliable cell-based assays and validated analysis systems. In this seminar, a variety of techniques for bacterial and viral analysis will be demonstrated in cell-based models using powerful cytometric instrumentation. Special focus will be given to assays to characterize bacteria and the immune response to bacteria. Additionally, discussion will include methods to evaluate viral infection and proliferation in host cells. Recent innovations in high-throughput flow cytometry, partnered with regulatory-compliant acquisition and analysis software, will provide valuable additions to your portfolio of assays for microbial research.

Basics of Building an Intracellular Flow Cytometry Panel

Let’s get ready to immunophenotype! Panel building usually requires many steps for optimal experimental design. In this session, learn the 5 steps for building a quick and easy experiment to find your immune cell subsets.

Antibody-Mediated Immune Cell Effector Function in Whole Blood

Monoclonal antibody therapeutics have proven to be a robust and effective modality to treat a range of diseases. Many therapeutic antibodies such as Rituximab have seen clinical success in treating cancer. Though some antibodies can kill cancer cells directly, the primary mechanism of action is often via antibody-dependent killing by immune cells or complement protein complexes in the blood. In vitro assays to characterize antibody-dependent cell killing commonly rely on preparations of isolated immune cells such as PBMCs or isolated T- or NK-cells. While these models provide a reliable cell-based system to characterize antibody function, they may be biased by the absence of components present in whole blood. We describe new flow cytometry methods to characterize immune cell killing and use these assays to evaluate antibody-dependent killing of leukemia cells with isolated T and NK cells compared to whole blood.

Introduction to Spectral Flow Cytometry and Panel Design

The continued development of spectral cytometers in the last few years is enabling expanded multiplexing in cellular analysis by harnessing the full emission spectrum of fluorophores used in flow cytometry. Full emission spectral analysis is not a new concept, but due to technological bottlenecks, it was not generally adopted until recently. Spectral cytometers can leverage the unique spectral signatures found in the novel dye portfolio that Thermo Fisher Scientific has developed. This seminar will briefly explore the difference between conventional flow cytometry and spectral flow cytometry. We will also explore the importance of single-color controls, the possible combinations of dyes currently in the market, and panel design.

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