Fundamentals of Fluorescence Microscopy
Learn about basic properties of light, fluorophore excitation, emission, and Stokes shift.
Understand advantages of fluorescence microscopy compared to brightfield, the differences between magnification and resolution, and how using fluorescence can help increase resolution.
Learn about the light path in typical epifluorescence microscopes and their limits of resolution
Understand why filters are used and where in the light path you will find them.
Understand what the code on your objective means, why numerical aperture matters, and ways to make sure your microscope configuration will work for your experiment