|Many times science can be 1% inspiration, 99% iteration. Part of getting good results is reviewing your less-than-perfect outcomes and figuring out what to try next time. Learn about some of the problems you may encounter, see examples of what it looks like when things go wrong, and get hints for preventing those same problems in the future.|
Learn ways to reduce background (or noise)—the nonspecific fluorescent signal coming from ambient light, the microscope, or the experiment components.
Fluorescence that starts out bright but dims over time may be because the fluorophore is being degraded by light (photobleaching). Learn how to minimize it.
When two or more fluorophores are used, light from one fluorophore can “contaminate” the channel of the other. Learn ways to minimize this bleed-through.
Learn the sources of phototoxicity, how to recognize stressed cells, and ways to reduce this kind of damage in your live-cell imaging experiments.