T cell activation with Dynabeads

T cell activation with Dynabeads

Isolate, activate, and expand your T cells with Dynabeads products. Ensure viability of your isolated T cells with our trusted and highly referenced Invitrogen and Gibco Dynabeads magnetic beads. Stimulate the CD3/TCR and CD28 signaling pathways, using Gibco Dynabeads magnetic beads coated with anti-CD3 and anti-CD28 antibodies. This workflow enables the isolation, activation, and expansion of robust T cells while maintaining cell viability for downstream immuno-oncology analysis.

Choose Dynabeads, it is the fastest growing T cell activation technology​ and the new gold standard in the market.

  • Physiological activation
  • Highly consistent activation and expansion
  • No need for autologous Antigen Presenting Cells (APCs), mitogens, antigens, or feeder cells
  • Cytokine profile and a full T cell Receptors (TCR) repertoire is retained
  • Available for both human and mouse T cells
  • Method translates from mouse studies to human immunotherapy on the same platform

Activation or expansion of polyclonal T cells

Dynabeads T cell activation and expansion products mimic in vivo T cell activation from antigen-presenting cells (APCs)

The CD3 and CD28 antibodies on the Dynabeads surface simultaneously bind the CD3 and CD28 antigens on the target cells, thus initiating the signaling pathway. The antibodies are coupled onto 4.5 um sized beads (almost as large as APC’s), and this 3-dimentional structure enables activation very similar to the physiological activation happening in the body. The optimized antibody amount and the reproducibility of the product results in a more consistent activation versus more primitive options like mitogens or soluble antibodies.

The protocols are straightforward, highly consistent, and do not require autologous APC, mitogens, antigen or feeder cells. The Dynabeads technology outperforms traditional home-brew methods for generic activation and is highly cited in the peer reviewed publications. Importantly, you can even use the same technology platform when moving from mouse or human studies to clinical research.

Key benefits

  • Ease-of-use: No need to prepare autologous APC, feeder cells, or antigen. No need to pre-coat antibodies to beads.
  • Physiological activation: Simultaneous signals with agonistic monoclonal antibodies to CD3/TCR and CD28.
  • Stimulate and re-stimulate: Release and remove the magnetic beads. Cells can be re-stimulated several times.
  • Reliability: Cells keep their functionality with a cytokine profile and a full T cell repertoire reflecting that of in vivo settings.

Main applications

The Dynabeads technology allows you to activate and/or expand naïve and memory T cells, T cell clones, regulatory T cells, NKT cells and CTLs from mouse and human samples.

The technology allows for the activation of T cells for signaling experiments (proteomics), T cell expansion, T helper cell differentiation (Th1, Th2, Th17), expansion of regulatory T cells and T cell activation for improved transduction efficiency of MNC/T cells. As research needs change towards clinical, Dynabeads cGMP CTS beads allow for the seamless move from mouse studies to clinical research using the same technology platform.

Dynabeads T-Activator CD3/CD28 products for human and mouse are ideal for short time stimulation studies and T cell expansion of polyclonal T cells (e.g T cell subsets isolated from whole blood, mononuclear cells or tissue digests).

Hear what some of our valued customers say about the cell activation beads:

"I used the CD3/CD28 Dynabeads for my proliferation experiment, and the experiment worked beautifully!
I have been trying to get to work for a couple of months using soluble CD28/CD3, and it was just not happening. Dynabeads have definitely made my day!"

Lisa S. Thomas, Research Associate IV, Cedars-Sinai Medical Center

Efficient expansion of Ag-specific T cells

The main application for these products is T cell re-stimulation and expansion of antigen-specific (Ag-specific) T cells from cell lines, cell clones or freshly isolated/enriched Ag-specific T cells.

Key benefits

  • Physiological activation: Stimulation of CD3/TCR, CD28 and CD137 with agonistic monoclonal antibodies
  • Efficiency: Increased expansion and survival of Ag-specific T cells
  • Reliability: T cells expanded with Dynabeads maintain Ag-specificity when tested in bioassays
  • Ease- of-use: No need to find or prepare donor matched APCs or antigen

To allow for expansion of human antigen-specific T cells, the Dynabeads are conjugated with anti-CD3, anti-CD28, and anti-CD137 antibodies. CD137 (4-1BB) is a member of the tumor necrosis factor family and agonistic anti-CD137 antibodies act as an activating co-stimulatory molecule especially important for effector/ memory T cells and promote the survival and proliferation of T lymphocytes. 

This technology allows you to activate and/or expand naïve and memory T cells, T cell clones, regulatory T cells, natural killer T (NKT) cells and cytoxic T lymphocytes (CTLs) from mouse and human samples. A clinical research grade product is also available, allowing you to move from mouse studies to clinical research using the same technology platform.

The new Dynabeads T-Activator CD3/CD28/CD137 for expansion of human antigen-specific T cells are conjugated with anti-CD3, anti-CD28, and anti-CD137 antibodies. CD137 (4-1BB) is a member of the tumor necrosis factor family and agonistic anti-CD137 antibodies act as an activating co-stimulatory molecule especially important for effector/ memory T cells and promote the survival and proliferation of T lymphocytes.

Natural Activation of Ag-specific T cell

The Dynabeads technology allows you to activate and/or expand naïve and memory T cells, T cell clones, regulatory T cells, NKT cells and CTLs from mouse and human samples.

Our full portfolio of products also include a clinical research grade version, allowing you to move from mouse studies to clinical research using the same technology platform.

Ag-specific T cell activation using Dynabeads

Faster growing T cell activation method

Generally, interest in T cell activation methods have not changed much for Generic mitogen-based T cell activation (home-brew), Soluble and/or plate-bound antibodies, and Antigen presenting cells (APC’s) from 2012 to 2018. However, during this same period, publications citing the use of Dynabeads magnetic beads have increased dramatically.

2018 Publication Trend T Cell Activation
Published papers on T cell activation.
  • Physiological activation
  • Highly consistent activation and expansion
  • No need for autologous Antigen Presenting Cells (APCs), mitogens, antigens, or feeder cells
  • Cytokine profile and a full T cell Receptors (TCR) repertoire is retained
  • Available for both human and mouse T cells
  • Method translates from mouse studies to human immunotherapy on the same platform

Activation or expansion of polyclonal T cells

Dynabeads T cell activation and expansion products mimic in vivo T cell activation from antigen-presenting cells (APCs)

The CD3 and CD28 antibodies on the Dynabeads surface simultaneously bind the CD3 and CD28 antigens on the target cells, thus initiating the signaling pathway. The antibodies are coupled onto 4.5 um sized beads (almost as large as APC’s), and this 3-dimentional structure enables activation very similar to the physiological activation happening in the body. The optimized antibody amount and the reproducibility of the product results in a more consistent activation versus more primitive options like mitogens or soluble antibodies.

The protocols are straightforward, highly consistent, and do not require autologous APC, mitogens, antigen or feeder cells. The Dynabeads technology outperforms traditional home-brew methods for generic activation and is highly cited in the peer reviewed publications. Importantly, you can even use the same technology platform when moving from mouse or human studies to clinical research.

Key benefits

  • Ease-of-use: No need to prepare autologous APC, feeder cells, or antigen. No need to pre-coat antibodies to beads.
  • Physiological activation: Simultaneous signals with agonistic monoclonal antibodies to CD3/TCR and CD28.
  • Stimulate and re-stimulate: Release and remove the magnetic beads. Cells can be re-stimulated several times.
  • Reliability: Cells keep their functionality with a cytokine profile and a full T cell repertoire reflecting that of in vivo settings.

Main applications

The Dynabeads technology allows you to activate and/or expand naïve and memory T cells, T cell clones, regulatory T cells, NKT cells and CTLs from mouse and human samples.

The technology allows for the activation of T cells for signaling experiments (proteomics), T cell expansion, T helper cell differentiation (Th1, Th2, Th17), expansion of regulatory T cells and T cell activation for improved transduction efficiency of MNC/T cells. As research needs change towards clinical, Dynabeads cGMP CTS beads allow for the seamless move from mouse studies to clinical research using the same technology platform.

Dynabeads T-Activator CD3/CD28 products for human and mouse are ideal for short time stimulation studies and T cell expansion of polyclonal T cells (e.g T cell subsets isolated from whole blood, mononuclear cells or tissue digests).

Hear what some of our valued customers say about the cell activation beads:

"I used the CD3/CD28 Dynabeads for my proliferation experiment, and the experiment worked beautifully!
I have been trying to get to work for a couple of months using soluble CD28/CD3, and it was just not happening. Dynabeads have definitely made my day!"

Lisa S. Thomas, Research Associate IV, Cedars-Sinai Medical Center

Efficient expansion of Ag-specific T cells

The main application for these products is T cell re-stimulation and expansion of antigen-specific (Ag-specific) T cells from cell lines, cell clones or freshly isolated/enriched Ag-specific T cells.

Key benefits

  • Physiological activation: Stimulation of CD3/TCR, CD28 and CD137 with agonistic monoclonal antibodies
  • Efficiency: Increased expansion and survival of Ag-specific T cells
  • Reliability: T cells expanded with Dynabeads maintain Ag-specificity when tested in bioassays
  • Ease- of-use: No need to find or prepare donor matched APCs or antigen

To allow for expansion of human antigen-specific T cells, the Dynabeads are conjugated with anti-CD3, anti-CD28, and anti-CD137 antibodies. CD137 (4-1BB) is a member of the tumor necrosis factor family and agonistic anti-CD137 antibodies act as an activating co-stimulatory molecule especially important for effector/ memory T cells and promote the survival and proliferation of T lymphocytes. 

This technology allows you to activate and/or expand naïve and memory T cells, T cell clones, regulatory T cells, natural killer T (NKT) cells and cytoxic T lymphocytes (CTLs) from mouse and human samples. A clinical research grade product is also available, allowing you to move from mouse studies to clinical research using the same technology platform.

The new Dynabeads T-Activator CD3/CD28/CD137 for expansion of human antigen-specific T cells are conjugated with anti-CD3, anti-CD28, and anti-CD137 antibodies. CD137 (4-1BB) is a member of the tumor necrosis factor family and agonistic anti-CD137 antibodies act as an activating co-stimulatory molecule especially important for effector/ memory T cells and promote the survival and proliferation of T lymphocytes.

Natural Activation of Ag-specific T cell

The Dynabeads technology allows you to activate and/or expand naïve and memory T cells, T cell clones, regulatory T cells, NKT cells and CTLs from mouse and human samples.

Our full portfolio of products also include a clinical research grade version, allowing you to move from mouse studies to clinical research using the same technology platform.

Ag-specific T cell activation using Dynabeads

Faster growing T cell activation method

Generally, interest in T cell activation methods have not changed much for Generic mitogen-based T cell activation (home-brew), Soluble and/or plate-bound antibodies, and Antigen presenting cells (APC’s) from 2012 to 2018. However, during this same period, publications citing the use of Dynabeads magnetic beads have increased dramatically.

2018 Publication Trend T Cell Activation
Published papers on T cell activation.

Ordering information

Dynabeads T Cell Activation Steps

Tour of an activated T Cell with Dynabeads