Dynabeads T cell activation and expansion products mimic in vivo T cell activation.

Dynabeads mimic in vivo T cell activation from antigen-presenting cells (APCs) and provide simultaneous signals to TCR/CD3 and CD28.

No need for autologous APC, mitogens, antigen or feeder cells.
This technology out-performs traditional home-brew methods for generic activation (mitogens, ConA, soluble antibodies etc.,) and is well documented in the published literature.

Physiological activation of mouse/human T cells using Dynabeads is highly consistent.

You can even use the same technology platform when moving from mouse or human studies to clinical research.

* Larger volume-sizes (2 ml and 10 ml) are also available. Note that these larger volumes are sold under the name Dynabeads CD3/CD28 T Cell Expander (Cat. no. 11-31D and 111-32D) and Dynabeads Mouse CD3/CD28 T Cell Expander  (Cat. no. 114-52D and 114-53D).

Selected References

  1. Berger, C. et al. (2003) CD28 costimulation and immunoaffinity-based selection efficiently generate primary gene-modified T cells for adoptive immunotherapy. Blood 101:476–484.
  2. Bonyhadi, M. et al. (2005) In vitro engagement of CD3 and CD28 corrects T cell defects in chronic lymphocytic leukemia. J Immunol 174:2366–2375.
  3. Hami, L. et al. (2003) Comparison of a static process and a bioreactor-based process for the GMP manufacture of autologous Xcellerated T cells for clinical trials. BioProcessing Journal Vol. 2 No. 6, November/December 2003.
  4. Levine, B.L. et al. (2002) Adoptive transfer of costimulated CD4+ T cells induces expansion of peripheral T cells and decreases CCR5 expression in HIV infection. Nat Med 8:47–53.
  5. Godfrey, W.R. et al. (2004) In vitro expanded human CD4+CD25+ T regulatory cells can markedly inhibit allogeneic dendritic cell stimulated MLR cultures. Blood 104:453–461.
  6. Coito, S. et al. (2004) Retrovirus-mediated gene transfer in human primary T lymphocytes induces an activation-and transduction/selection-dependent TCRBV repertoire skewing of gene-modified cells. Stem Cells Dev 13:71–81.
  7. Rapoport, A.P. et al. (2005) Restoration of immunity in lymphopenic individuals with cancer by vaccination and adoptive T-cell transfer. Nat Med 11:1162–1163.
  8. Kalamasz, D. et al. (2004) Optimization of Human T-Cell Expansion Ex Vivo Using Magnetic Beads Conjugated with Anti-CD3 and Anti-CD28 Antibodies (Clinical Studies). J Immunother 27(5):405-418.
  9. Levine, BL. et. al. (2002) Adoptive transfer of costimulated CD4+ T cells induces expansion of peripheral T cells and decreased CCR5 expression in HIV infection. Nat Med 8:47-53.
  10. Earle, KE. et. al. (2005) In vitro expanded human CD4+CD25+ regulatory T cells suppress effector T cell proliferation. Clin Immunol 115(1):3-9.