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Cell Isolation with |
Dynabeads magnetic cell separation technology
Gentle, tube-based magnetic separation with Invitrogen Dynabeads or MagniSort beads are technologies of choice when you want to isolate high yields of pure, viable, and functional cells. Magnetic separation helps ensure that the isolated cells are not affected by passage through a dense column and that the cells’ native characteristics are preserved.
Positive cell isolation with Dynabeads
In positive cell isolation, Dynabeads bind the target cells and the bead/cell complexes are pulled to the magnet. The supernatant is discarded, leaving only your pure target cells.
Bead-free cells
Directly after cell separation, the beads can easily be released and removed using the Dynabeads FlowComp technology.
Negative cell isolation with Dynabeads
In negative cell isolation, Dynabeads are used to deplete all the unwanted cells using the supplied antibody mix. Your bead-free and untouched target cells are left in the sample, and can be directly analyzed in a flow cytometer and used in any functional assay.
To refine for a particular cell type, you can of course add extra antibodies to the antibody mix. With the secondary antibody-coated Dynabeads sheep anti-rabbit IgG and sheep anti-mouse IgG you can create your own negative isolation kit for isolation of any cells from any species.
- Learn more about negative cell separation
Depletion with Dynabeads
In depletion, cell-specific Dynabeads are used to remove specific unwanted cell types from a mixed cell sample with >99% efficiency. Recover the depleted supernatant—containing all your cells of interest—for downstream assays.
Magnetic beads for other cell isolation
You can also use the positively targeted and bead-bound cells for downstream molecular applications that do not require release of cells.
Automated cell isolation with Dynabeads and KingFisher systems
Automate your cell isolation on the Thermo Scientific KingFisher family of instruments. KingFisher instruments have the ability to eliminate any cross contamination, have reproducible results, and cut your hands-on time down to 40 min. Set up plates, press start, and walk away.
Cell isolation protocols for KingFisher Duo Prime instrument
| Ligand | Product | Protocol description | Strategy | Download file |
|---|---|---|---|---|
| Cells | CD3, CD4, CD8, CD14, CD19 | Positive Cell Isolation for downstream nucleic acid, protein analysis, or cell depletion | Indirect | Duo Prime |
| Cells | CD3, CD4, CD8 | Positive Cell Isolation for downstream cellular applications—such as T-cell activation | Indirect | Duo Prime |
Positive cell isolation with Dynabeads
In positive cell isolation, Dynabeads bind the target cells and the bead/cell complexes are pulled to the magnet. The supernatant is discarded, leaving only your pure target cells.
Bead-free cells
Directly after cell separation, the beads can easily be released and removed using the Dynabeads FlowComp technology.
Negative cell isolation with Dynabeads
In negative cell isolation, Dynabeads are used to deplete all the unwanted cells using the supplied antibody mix. Your bead-free and untouched target cells are left in the sample, and can be directly analyzed in a flow cytometer and used in any functional assay.
To refine for a particular cell type, you can of course add extra antibodies to the antibody mix. With the secondary antibody-coated Dynabeads sheep anti-rabbit IgG and sheep anti-mouse IgG you can create your own negative isolation kit for isolation of any cells from any species.
- Learn more about negative cell separation
Depletion with Dynabeads
In depletion, cell-specific Dynabeads are used to remove specific unwanted cell types from a mixed cell sample with >99% efficiency. Recover the depleted supernatant—containing all your cells of interest—for downstream assays.
Magnetic beads for other cell isolation
You can also use the positively targeted and bead-bound cells for downstream molecular applications that do not require release of cells.
Automated cell isolation with Dynabeads and KingFisher systems
Automate your cell isolation on the Thermo Scientific KingFisher family of instruments. KingFisher instruments have the ability to eliminate any cross contamination, have reproducible results, and cut your hands-on time down to 40 min. Set up plates, press start, and walk away.
Cell isolation protocols for KingFisher Duo Prime instrument
| Ligand | Product | Protocol description | Strategy | Download file |
|---|---|---|---|---|
| Cells | CD3, CD4, CD8, CD14, CD19 | Positive Cell Isolation for downstream nucleic acid, protein analysis, or cell depletion | Indirect | Duo Prime |
| Cells | CD3, CD4, CD8 | Positive Cell Isolation for downstream cellular applications—such as T-cell activation | Indirect | Duo Prime |
MagniSort cell isolation products
Starting with T cell-specific cocktails, use the MagniSort platform to obtain enriched T cells simpler, faster, and with significant cost-savings compared to traditional column-based separation methods.
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