Which Mouse CD8+ T Cell Isolation Product is Right for You?

  Affordable & pure, flow compatible cells Highest purity for molecular assays Untouched—most physiological relevant cells
Isolation methodPositive isolationDepletion or positive isolationNegative isolation
Starting samplesAny sample (whole blood, spleen, lymph node)Any sample (whole blood, spleen, lymph node)Spleen, lymph node cells*
# Cells processed2 x 109 cells 1-2 x 109 cells 1 x 109 cells
PurityHigh
>90%
High
>90%
Standard
>70%
YieldStandard
>60%
Highest
>90%
Standard
>60%
Viability>90%>90%>90%
Isolation time~60min~30 min~50 min
Isolation temperatureColdColdCold
ApplicationsCellular assays
(cell culture, cell activation/ proliferation, cytokine studies)
Molecular:
isolation of proteins & nucleic acids
Depletion of unwanted CD8+ T cells or positive isolation for molecular isolation of proteins & nucleic acidsCellular applications for physiological studies (functional studies, functional studies, adoptive transfer, flow analysis or further sorting)
Bead-free target cellsYes (but antibody present)NoYes
Compatible with flow cytometry /flow sortingyes yes
Shelf life from production date2 years3 years3 years
Required materialsMagnetsMagnetsMagnets
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* Lyse the red cells prior to use to improve the purity
THIS IS FOR MOUSE RESEARCH - NOT FOR HUMAN
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