Human Dendritic Cell Enrichment and Recovery

Dendritic cells play a critical role in the detection of pathogens and activation of the adaptive immune response. Dendritic cells initiate the adaptive response by presenting antigenic peptides on major histocompatibility complex (MHC) molecules to induce T cell activation and differentiation. In addition to their role in activating naïve T cells, Dendritic cells guide the differentiation of regulatory T cells as well as the development of T cell tolerance. As key sentinel cells, Dendritic cells reside throughout the body, particularly in lymphoid organs and at environmental interfaces such as the intestine and skin.

Increase your recovery of subsets of dendritic cells by using Dynabeads Human DC Enrichment kit in combination with flow sorting. T cells, B cells, monocytes/ macrophages, granulocytes, NK cells and erythrocytes are depleted from mononuclear cells (MNC) to leave a bead- and antibody free population of cells highly enriched for dendritic cells.

You can expect an 80-100% recovery of the Lin- CD4+ cells, untouched and viable. Enriched dendritic cells can be further isolated to both myeloid and plasmacytoid dendritic cell subpopulation by flow sorting.

With this gentle depletion of non-dendritic cells, your sorting time will be reduced by a factor of typically 10-15 compared to sorting only. The additional benefit is that you can reduce the need of fluorescent antibody for labeling of cells. Pre-enrichment thus saves both money and time!

For successful dendritic cell isolation the Dynabeads Human DC Enrichment kit should be used with the magnets MPC-L, MPC-50 or MPC-15.