Fusion of a lysosome with the isolation membrane to form the autolysosome is the penultimate step of the autophagic pathway. Lysosomal membrane markers serve as tools for tracking fusion with the autophagosome prior to degradation of the autolysosome contents, whereas cell permeable LysoTracker® dyes target acidic organelles and are localized within the lysosomes in nanomolar concentrations. Both membrane proteins and Lysotracker dyes serve as effective tools for tracking lysosome behavior.
Autophagy describes the segregation and delivery of cytoplasmic cargo, including proteins and organelles, for degradation by hydrolytic enzymes through the lysosomal machinery. It is critical to the healthy functioning of cells and the failure of autophagy is a major reason for the accumulation of cell damage and aging.
Lysosomal membrane markers serve as tools for tracking fusion with the autophagosome prior to degradation of the autolysosome contents. We offer Molecular Probes® CellLight® constructs containing red or green fluorescent proteins for locating and tracking lysosomal membranes.
Human osteosarcoma (U2OS) cells expressing CellLight® Lysosomes-GFP.
Cell-permeable Molecular Probes® LysoTracker® dyes target acidic organelles in nanomolar concentrations. They are available in a range of colors including deep red to facilitate multiplexing with other fluorescent markers.
Co-localization using LysoTracker® Deep Red.
|Readout||Localization of lysosomes by fluorescent protein imaging|
|Standard filter set||FITC||TRITC|
|Format||1 kit||1 kit|
|Target||Targets acidic organelles at nanomolar concentrations|
|Readout||Localization of lysosomes by fluorescence imaging|
|Fluorescent label||LysoTracker® Green||LysoTracker® Red||LysoTracker® Deep Red|
|Standard filter set||FITC||TRITC||Cy®5|
|Format||20 x 50 uL||20 x 50 uL||5 x 50 uL|
|Molecular Probes® Handbook