Cell cycle analysis

Mitotic arrest is frequently used as an indicator of cytotoxicity in mammalian cells, and measures of mitotic index can be used to evaluate compounds during drug discovery and development. Life Technologies offers tools based on antibody detection or transgenic protein expression to monitor cell cycle progression.

Live or fixed cell analysis

There is a tight correlation between chromosome condensation and histone 3 (Ser10) phosphorylation during mitosis. This event can serve as an indication of mitotic progression or inhibition within the context of drug profiling.

The fluorescence ubiquitination cell cycle indicator (FUCCI) is a genetically encoded, two-color (red and green) reporter for following cell division within a cell population.

Both the HCS Mitotic Index Kit and the Premo™ FUCCI Cell Cycle Sensor can be used to monitor the effects of a compound on the cell cycle while multiplexing with other measurements such as cytotoxicity or immunodetection of choice targets.

  Cell cycle analysis
Visualization of cell cycle phases with the Premo™ FUCCI Cell Cycle Sensor over a 16 hour period.

Selection guide for cell cycle analysis using imaging

 
Readout Anti–phosphohistone H3 serves as a sensitive indicator of mitosis and is detected with Alexa Fluor® 488 secondary antibody As a cell progresses through the cell cycle, fluorescence of the Premo™ FUCCI sensor changes from red to green
Common filter set FITC RFP FITC
Reporter Alexa Fluor® 488 dye RFP GFP
Ex/Em (nm) 495/519 555/584 485/520
Live-cell compatible No Yes
Stains fixed cells Yes No
Formaldehyde fixable Yes Yes
Multiplexing Yes Yes
Signal-to-noise 5 stars 4 stars
Photostability 5 stars 4 stars
Platform Imaging, HCS Imaging, HCS
Bibliography Citations
Format 1 kit/2 microplates 2 x 200 μL 2 x 1,000 μL
Cat. No. H10293 P36238 P36237