Mitotic arrest is frequently used as an indicator of cytotoxicity in mammalian cells, and measures of mitotic index can be used to evaluate compounds during drug discovery and development. We offer tools based on transgenic protein expression to monitor cell cycle progression.

Select products for cell cycle analysis using imaging
Select products for cell cycle analysis using flow cytometry

 

 

Live-cell cell cycle analysis

There is a tight correlation between chromosome condensation and histone 3 (Ser10) phosphorylation during mitosis. This event can serve as an indication of mitotic progression or inhibition within the context of drug profiling.

The fluorescence ubiquitination cell cycle indicator (FUCCI) is a genetically encoded, two-color (red and green) reporter for following cell division within a cell population.

The Premo FUCCI Cell Cycle Sensor can be used to monitor the effects of a compound on the cell cycle while multiplexing with other measurements such as cytotoxicity or immunodetection of choice targets.

  Cell cycle analysis

Visualization of cell cycle phases with the Premo FUCCI Cell Cycle Sensor over a 16 hour period.

Selection guide for cell cycle analysis using imaging

 
Readout As a cell progresses through the cell cycle, fluorescence of the Premo FUCCI sensor changes from red to green
Common filter set RFP FITC
Reporter RFP GFP
Ex/Em (nm) 555/584 485/520
Live-cell compatible Yes
Stains fixed cells No
Formaldehyde fixable Yes
Multiplexing Yes
Signal-to-noise 4 stars
Photostability 4 stars
Platform Imaging, HCS
Bibliography  
Format 2 x 200 μL 2 x 1,000 μL
Cat. No. P36238 P36237