Mitotic arrest is frequently used as an indicator of cytotoxicity in mammalian cells, and measures of mitotic index can be used to evaluate compounds during drug discovery and development. We offer tools based on transgenic protein expression to monitor cell cycle progression.
There is a tight correlation between chromosome condensation and histone 3 (Ser10) phosphorylation during mitosis. This event can serve as an indication of mitotic progression or inhibition within the context of drug profiling.
The fluorescence ubiquitination cell cycle indicator (FUCCI) is a genetically encoded, two-color (red and green) reporter for following cell division within a cell population.
The Premo FUCCI Cell Cycle Sensor can be used to monitor the effects of a compound on the cell cycle while multiplexing with other measurements such as cytotoxicity or immunodetection of choice targets.
Visualization of cell cycle phases with the Premo FUCCI Cell Cycle Sensor over a 16 hour period.
|Readout||As a cell progresses through the cell cycle, fluorescence of the Premo FUCCI sensor changes from red to green|
|Common filter set||RFP||FITC|
|Stains fixed cells||No|
|Format||2 x 200 μL||2 x 1,000 μL|
|Molecular Probes Handbook