Phospholipidosis and steatosis are toxic side effects of lipid metabolism that can be triggered by drugs or other compounds. Phospholipidosis is a lysosomal storage disorder associated with cationic amphiphilic drugs and characterized by the accumulation of excess phospholipid complexes within the internal lysosomal membranes. Steatosis is the abnormal retention of lipids within a cell due to impairment of the normal processes of synthesis and elimination of triglyceride fats.

 

HCS LipidTOX™ stains

HCS LipidTOX™ neutral lipid stains were developed for image-based high-content screening (HCS) assays to characterize the potentially toxic side effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX™ assay series includes neutral lipid stains for fixed cells and phospholipid stains for live cells, which can be combined in multiplexed assays.

  HCS LipidTOX™ Stains
Lipid vesicles in bone marrow—derived hMSCs visualized with LipidTOX™ Green neutral lipid stain and counterstained with Hoechst 33342.

Selection guides for HCS LipidTOX™ stains

 
Readout High affinity for neutral lipid droplets in fixed cells
Common filter set Cy®5 Texas Red® FITC
Reporter LipidTOX™ Deep Red neutral lipid stain LipidTOX™ Red neutral lipid stain LipidTOX™ Green neutral lipid stain
Ex/Em (nm) 637/655 577/609 495/505
Live-cell compatible No
Added to growth media No
Formaldehyde fixable Cells labeled after fixation
Multiplexing Can be multiplexed with phospholipidosis detection reagents
(see tables below)
Platform Imaging, HCS
Bibliography Citations
Format 125 μL (1,200 assays for steatosis/240 assays for adipogenesis)
Cat. No. H34477 H34476 H34475
 
Readout Kit for the sequential analysis of phospholipidosis and steatosis
Common filter set Texas Red® FITC
Reporter LipidTOX™ Red phospholipid stain LipidTOX™ Green neutral lipid stain
Ex/Em (nm) 595/615 495/505
Live-cell compatible Yes No
Added to growth media Yes No
Formaldehyde fixable Yes Labeled after fixation
Multiplexing Combination assay kit for HCS
Bibliography Citations
Platform Imaging, HCS
Format 2 plates/240 assays 10 plates/1,200 assays
Cat. No. H34157 H34158
 
Readout Labels phospholipid accumulation after incubation with live cells
Common filter set FITC Texas Red®
Reporter LipidTOX™ Green phospholipid stain LipidTOX™ Red phospholipid stain
Ex/Em (nm) 495/525 595/615
Live-cell compatible Yes
Added to growth media Yes
Formaldehyde fixable Yes
Multiplexing Can be multiplexed with neutral lipid stains (see table above)
Bibliography Citations
Platform Imaging, HCS
Format 125 μL (1,200 assays)
Cat. No. H34350 H34351

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