Invitrogen EVOS Light Cubes integrate LEDs and high-performance filters in a novel illumination system that delivers precise control, minimal maintenance, and exceptional reliability.
These interchangeable LED cubes provide:
- Shorter light paths for more efficient fluorescent detection
- Longer bulb lifetime (>50,000 hours) for lower maintenance costs
- Adjustable light intensity to minimize photobleaching
- Continuous light intensity for more consistent results
These LED cubes are interchangeable, have plug-and-play capability and can be easily transferred between the Invitrogen Countess II FL, EVOS FL, and Invitrogen EVOS FL Auto 2 Cell Imaging System.
|Light Cube*||Ex (nm)||Em (nm)||Key Compatible Dyes
|DAPI||357/44||447/60||DAPI, Hoechst, BFP||AMEP4650|
|CFP||445/45||510/42||ECFP, lucifer yellow||AMEP4653|
|GFP||470/22||525/50||GFP, Invitrogen Alexa Fluor 488, FITC, Applied Biosystems SYBR Green||AMEP4651|
|YFP||500/24||542/27||EYFP, acridine orange (+ DNA)||AMEP4654|
|RFP||531/40||593/40||RFP, Invitrogen Alexa Fluor 546, Alexa Fluor 555, Cy3, DsRed, Rhodamine Red||AMEP4652|
|Invitrogen Texas Red||585/29||628/32||Invitrogen Texas Red, Alexa Fluor 568, Alexa Fluor 594, MitoTracker Red, mCherry, Cy3.5||AMEP4655|
|Invitrogen Cy5||628/40||685/40||Invitrogen Cy5, Alexa Fluor 647, Alexa Fluor 660, DRAQ5||AMEP4656|
|Invitrogen Cy7||710/40||809/81||Cy7, IRDye® 800CW||AMEP4667|
|CFP-YFP||445/45||542/27||CFP/YFP (for FRET applications)||AMEP4669|
|AO||470/22||488/20||Acridine orange (+RNA, simultaneous red/green with FL color)||AMEP4670|
|AOred||445/45||628/32||Acridine orange + RNA, CTC formazan, Invitrogen Fura Red (high Ca2+)||AMEP4671|
|Invitrogen Qdot 525–800||445/45||515/LP||All Invitrogen Qdot nanocrystals||AMEP4666|
|Qdot 525||445/45||525/15||Qdot 525||AMEP4657|
|Qdot 545||445/45||544/24||Qdot 545||AMEP4658|
|Qdot 565||445/45||567/15||Qdot 565||AMEP4659|
|Qdot 585||445/45||588/20||Qdot 585||AMEP4660|
|Qdot 605||445/45||605/15||Qdot 605||AMEP4661|
|Qdot 625||445/45||625/15||Qdot 625||AMEP4662|
|Qdot 655||445/45||655/15||Qdot 655||AMEP4663|
|Qdot 705||445/45||710/40||Qdot 705||AMEP4664|
|Qdot 800||445/45||785/62||Qdot 800||AMEP4665|
* Click the Light Cube link (Cat. No.) for a complete list of compatible dyes/reagents.
The Power of LED Illumination
All of the EVOS fluorescence cell imaging systems utilize LED light sources. That means you get high-intensity output over a short light path for the most efficient fluorophore excitation.
- Shorter light path provides better detection of fluorescent signals
- Continuous illumination gives consistent results
- >50,000-hour bulb lifetime lowers your laboratory costs
- Adjustable light intensity reduces photobleaching
Revolutionary light path
By placing the LED light cube as close as possible to the objective turret, the number of optical elements in the light path is minimized. High-intensity illumination over a short light path increases the efficiency of fluorophore excitation, providing better detection of weak fluorescent signals.
Continuous light intensity
Mercury arc lamps can decrease in intensity by 50% in the first 100 hours of operation—plus, images acquired in different sessions cannot be quantitatively compared using mercury illumination without complicated calibrations. Because EVOS systems have continuous light cube intensity, users can rely on consistent illumination and can compare results quantitatively from images acquired on different days.
Less expensive to own and maintain
The LED bulbs on the EVOS systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb (and 1,500 hours for a metal halide bulb). That means significant savings in the overall upkeep of your instrument.
What light sources really cost
EVOS hard-coated filter sets for higher transmission efficiency
Hard-coated filter sets are more expensive, but they have sharper edges and significantly higher transmission efficiencies that typically result in >25% more light transmission than traditional soft-coated filters. With the EVOS system’s hard-coated filter sets, your light cubes cost less over time. Plus, you will have brighter fluorescence, higher transmission efficiencies, the ability to detect faint fluorescence signals, and better signal-to-noise ratios.
TRANSMISSION EFFICIENCY COMPARISON
Superior transmission efficiencies are observed using hard-coated filters on the EVOS instruments, compared to soft-coated filters. Excitation filter (purple), emission filter (red), dichroic mirror (green); Alexa Fluor 488 excitation (blue), Alexa Fluor 488 emission (pink).
For Research Use Only. Not for use in diagnostic procedures.