EVOS M5000 Imaging System

Revolutionizing your imaging routine

The EVOS M5000 Imaging System is a fully integrated digital inverted microscope for four-color fluorescence, transmitted-light and color applications. With an interface designed for usability, an advanced, yet flexible, illumination light system, sophisticated autofocus mechanism and high-resolution CMOS camera and time-lapse capabilities, you can acquire publication-quality images in minutes. The proprietary LED light cube and color imaging technologies allow capture of quantitative fluorescent and color samples, such as H&E-stained tissue slices, and make the EVOS M5000 system an exceptionally versatile microscope and ideal for a broad range of imaging applications.

Request a demoRequest a quote

The EVOS M5000 Imaging System offers you these important advantages:

  • Easy installation; no maintenance, assembly, alignment, or calibration
  • User defined four-color fluorescence, transmitted light and color applications
  • Autofocus, Z-stack capability, time-lapse imaging and single-click multi-channel capture
  • On-board software for fast acquisition and analysis, including confluency measurements and automated cell counting
  • Access to Thermo Fisher Cloud imaging app

Learn what you can now do with an EVOS M5000 Imager

Learn about software capability with M5000 Imaging System

Images captured using the EVOS M5000 Imaging System

Proliferation (EdU labeled) with Movat’s Pentachrome staining in FFPE 8 um rat intestine section

Figure 1. Proliferation (EdU labeled) with Movat’s Pentachrome staining in FFPE 8 μm rat intestine section. A transverse section through the crypts shows proliferating cell locally abundant near the base of the crypts and diminishing with distance. Proliferating cells (brown) signal is visible against Movat’s Pentachrome staining consisting of Verhoeff-Van Gieson’s staining of elastin (black) and nuclei (purplish gray), alcoholic saffron staining of collagen (yellow), alcian blue staining of mucins marking the goblet cells (blue), and crocein scarlet–acid fuchsin staining of muscle and red blood cells (red). Image was acquired with a 10X objective using the RGB-mode illumination on an EVOS M5000 microscope.

Primary Rat Cortical neurons cultured for 28 days in the B-27 Plus Neuronal Culture System

Figure 2. Primary rat cortical neurons cultured for 28 days in the B-27 Plus Neuronal Culture System. At day 28 cells were fixed and stained with antibodies against MAP2, synapsin1/2, and DAPI.

Live and heat-killed (60°C for 60 min) Jurkat cells were mixed together at a 50/50 ratio

Figure 3. Live and heat-killed (60°C for 60 min) Jurkat cells were mixed together at a 50/50 ratio. Cells were then stained with ReadyProbes Cell Viability Kit, Blue/Red. Cells were imaged on the EVOS M5000 microscope using 20x objective.

Transgenic fluorescent zebrafish (Fli1–enhanced Green Fluorescent Protein) including chorion, 24 hours post fertilization

Figure 4. Transgenic fluorescent zebrafish (Fli1–enhanced Green Fluorescent Protein) including chorion, 24 hours post fertilization. Embyros were fixed in 4% paraformaldehyde and then imaged in PBS using the EVOS M5000 microscope with a 4x phase objective. Phase image including GFP overlay is shown.

EVOS M5000 Imaging System Specifications

Optics Infinity corrected optical system; RMS threaded objectives with 45 mm parfocal distance
Illumination Adjustable intensity LED (>50,000 hour life per light cube); broad selection of patented standard and specialty light cubes
Light cubes (not included) Commonly used light cubes include (Ex/Em):
  • DAPI (357/447 nm),
  • GFP (470/525 nm),
  • RFP (531/593 nm),
  • Texas Red (585/624 nm),
  • Cy5 (628/692 nm)
Contrast methods Epifluoresence, transmitted light (brightfield and phase contrast) and RGB color illumination
Objective turret 5 position; front mounted control
Objectives (not included) Wide selection of high quality long working distance and coverslip corrected objectives
Condenser 60-mm long working distance condenser; 4-position turret with a clear aperture and 3 phase annuli
Focus mechanism Automated focus with sub micron (0.150 um) resolution (single step accuracy)
Mechanical focus wheel with single knob for coarse and fine focus
Stage Mechanical X/Y stage; travel range 120 mm x 80 mm with sub micron resolution, drop in inserts to receive vessel holders
LCD display 18.5" articulated LCD color monitor with 1920x1080 pixel resolution
Camera High-sensitivity 3.2 MP (2048 x 1536) monochrome CMOS sensor with 3.45 µm pixel resolution
Computer Embedded PC with 4 GB RAM
Captured images 16 bit monochrome TIFF or PNG (12 bit dynamic range); 8 bit color TIFF, PNG JPG and BMP
Storage 10 Gb SSD
16 Gb USB 3.0 memory stick
Output ports Power, 4 USB 2.0 ports, 1 USB 3.0 port, 1 Display Port, 1 RJ45 network jack
Networking capability Connection through Windows/SMB network via an Ethernet cable connection
USB 3.0 WiFi dongle
Power supply Universal Power Supply (12V, 5A) with Type B (North America) power cord*
Dimensions
(W x H x D)
18 x 23 x 18 inches
Weight 36 lb
* Note that one (1) country-specific power cord must be ordered separately in regions not using the Type B (North America) power plug:
AMEP4646 EVOS Power Cord, Type C (Europe)
AMEP4708 EVOS Power Cord, Type D (India/South Africa)
AMEP4645 EVOS Power Cord, Type G (United Kingdom)
AMEP4649 EVOS Power Cord, Type H (Israel)
AMEP4647 EVOS Power Cord, Type I (Australia)
AMEP4782 Power Cord, Type I (China, CCC certified)
AMEP4786 Power Cord Type J (Switzerland)
AMEP4648 EVOS Power Cord, Type L (Italy)

Images captured using the EVOS M5000 Imaging System

Proliferation (EdU labeled) with Movat’s Pentachrome staining in FFPE 8 um rat intestine section

Figure 1. Proliferation (EdU labeled) with Movat’s Pentachrome staining in FFPE 8 μm rat intestine section. A transverse section through the crypts shows proliferating cell locally abundant near the base of the crypts and diminishing with distance. Proliferating cells (brown) signal is visible against Movat’s Pentachrome staining consisting of Verhoeff-Van Gieson’s staining of elastin (black) and nuclei (purplish gray), alcoholic saffron staining of collagen (yellow), alcian blue staining of mucins marking the goblet cells (blue), and crocein scarlet–acid fuchsin staining of muscle and red blood cells (red). Image was acquired with a 10X objective using the RGB-mode illumination on an EVOS M5000 microscope.

Primary Rat Cortical neurons cultured for 28 days in the B-27 Plus Neuronal Culture System

Figure 2. Primary rat cortical neurons cultured for 28 days in the B-27 Plus Neuronal Culture System. At day 28 cells were fixed and stained with antibodies against MAP2, synapsin1/2, and DAPI.

Live and heat-killed (60°C for 60 min) Jurkat cells were mixed together at a 50/50 ratio

Figure 3. Live and heat-killed (60°C for 60 min) Jurkat cells were mixed together at a 50/50 ratio. Cells were then stained with ReadyProbes Cell Viability Kit, Blue/Red. Cells were imaged on the EVOS M5000 microscope using 20x objective.

Transgenic fluorescent zebrafish (Fli1–enhanced Green Fluorescent Protein) including chorion, 24 hours post fertilization

Figure 4. Transgenic fluorescent zebrafish (Fli1–enhanced Green Fluorescent Protein) including chorion, 24 hours post fertilization. Embyros were fixed in 4% paraformaldehyde and then imaged in PBS using the EVOS M5000 microscope with a 4x phase objective. Phase image including GFP overlay is shown.

EVOS M5000 Imaging System Specifications

Optics Infinity corrected optical system; RMS threaded objectives with 45 mm parfocal distance
Illumination Adjustable intensity LED (>50,000 hour life per light cube); broad selection of patented standard and specialty light cubes
Light cubes (not included) Commonly used light cubes include (Ex/Em):
  • DAPI (357/447 nm),
  • GFP (470/525 nm),
  • RFP (531/593 nm),
  • Texas Red (585/624 nm),
  • Cy5 (628/692 nm)
Contrast methods Epifluoresence, transmitted light (brightfield and phase contrast) and RGB color illumination
Objective turret 5 position; front mounted control
Objectives (not included) Wide selection of high quality long working distance and coverslip corrected objectives
Condenser 60-mm long working distance condenser; 4-position turret with a clear aperture and 3 phase annuli
Focus mechanism Automated focus with sub micron (0.150 um) resolution (single step accuracy)
Mechanical focus wheel with single knob for coarse and fine focus
Stage Mechanical X/Y stage; travel range 120 mm x 80 mm with sub micron resolution, drop in inserts to receive vessel holders
LCD display 18.5" articulated LCD color monitor with 1920x1080 pixel resolution
Camera High-sensitivity 3.2 MP (2048 x 1536) monochrome CMOS sensor with 3.45 µm pixel resolution
Computer Embedded PC with 4 GB RAM
Captured images 16 bit monochrome TIFF or PNG (12 bit dynamic range); 8 bit color TIFF, PNG JPG and BMP
Storage 10 Gb SSD
16 Gb USB 3.0 memory stick
Output ports Power, 4 USB 2.0 ports, 1 USB 3.0 port, 1 Display Port, 1 RJ45 network jack
Networking capability Connection through Windows/SMB network via an Ethernet cable connection
USB 3.0 WiFi dongle
Power supply Universal Power Supply (12V, 5A) with Type B (North America) power cord*
Dimensions
(W x H x D)
18 x 23 x 18 inches
Weight 36 lb
* Note that one (1) country-specific power cord must be ordered separately in regions not using the Type B (North America) power plug:
AMEP4646 EVOS Power Cord, Type C (Europe)
AMEP4708 EVOS Power Cord, Type D (India/South Africa)
AMEP4645 EVOS Power Cord, Type G (United Kingdom)
AMEP4649 EVOS Power Cord, Type H (Israel)
AMEP4647 EVOS Power Cord, Type I (Australia)
AMEP4782 Power Cord, Type I (China, CCC certified)
AMEP4786 Power Cord Type J (Switzerland)
AMEP4648 EVOS Power Cord, Type L (Italy)

Ordering information

Resources

EVOS Accessories

Cell Analysis Learning Center

Access cell analysis application resources for more success as you plan and execute your experiments.

Support

Cell Analysis Support Center

Find tips, troubleshooting help, and resources for your cell analysis workflows.